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Method for extracting beta-chitin from squid cartilage

A squid cartilage and chitin technology, applied in the field of biomaterials, can solve the problems of imperfect preparation conditions, unclear product parameters, easy configuration changes, etc., achieve superior moisture absorption performance, avoid product degradation, and reduce preparation costs.

Inactive Publication Date: 2019-11-15
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Molluscs such as squid cartilage mainly contain β-chitin. Due to the inedibility of squid cartilage, it is generally discarded as "waste" of seafood processing. Using squid cartilage to prepare β-chitin can not only prevent environmental pollution, but also achieve For the purpose of turning waste into treasure, the raw material resources are abundant and easy to obtain, with few impurities and stable output, but in the preparation process, there are disadvantages such as easy changes in configuration, imperfect preparation conditions, long time-consuming, and unclear product parameters.

Method used

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  • Method for extracting beta-chitin from squid cartilage
  • Method for extracting beta-chitin from squid cartilage

Examples

Experimental program
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Effect test

Embodiment 1

[0024] A method for extracting β-chitin from squid cartilage, comprising: using alkali boiling and ultrasonic waves to perform a protein removal process on the above squid cartilage, using acid leaching to perform a calcium removal process on the above squid cartilage after protein removal, and, further A process of providing concentrated alkali and porogen to solubilize the material obtained from calcium removal; the deacetylation degree of the soluble β-chitin product obtained from the above soluble treatment is 92-96%, and the nitrogen content is 6-10%. The method is to prepare soluble β-chitin through deproteinization and inorganic salt removal process of squid cartilage under the action of ultrasonic-assisted extraction, and then deacetylation reaction with concentrated alkali. The extraction process is simple and easy to operate, and the time is short. The amount of reagents is small, the raw materials are cheap and easy to obtain, the finished product has a high degree o...

Embodiment 2

[0034] A method for extracting β-chitin from squid cartilage, the specific preparation steps are as follows:

[0035] (1) After washing and drying the squid cartilage, crush it to 150 mesh fibrous powder;

[0036] (2) Add a sodium hydroxide solution with a concentration of 8wt% to the fibrous powder at a ratio of 1:6.5 to the ratio of solid to liquid, and keep it warm for 120 minutes at a temperature of 100 ° C. During this period, an ultrasonic wave with a power of 800 W is introduced every 20 minutes. , the single introduction time is 20min, until the alkali boiling is completed, then remove the solvent, wash the precipitate with water until neutral, and set aside;

[0037] (3) Add a hydrochloric acid solution with a concentration of 5.5wt% to the precipitate obtained in step (2) at a ratio of 1:10, soak for 5.5 hours at a temperature of 35°C, and wash with distilled water after soaking to neutral, then repeat the acid leaching operation once, and the decalcified product is...

Embodiment 3

[0040] The difference between this embodiment and Example 2 is only that the lye used in the protein removal process is a sodium hydroxide solution with a concentration of 8wt%, and the solid-liquid ratio of squid cartilage and lye is 1:4; the sodium hydroxide solution used 0.02-0.08 wt% of barbituric acid and 0.05-0.15 wt% of 4-methylguaiacol are also dispersed in it. The above-mentioned barbituric acid and 4-methylguaiacol can combine and remove the hydration electrons generated in the system under the action of ultrasonic waves, avoiding the recombination of hydration electrons and hydroxyl radicals, so as to improve the attack of chitin by hydroxyl radicals. The binding bond with protein makes the binding site between chitin and protein loose, can accelerate the degradation and separation of chitin and protein, can not only reduce the consumption of alkali, but also increase the purity of chitin, and the other two can be combined with The sugar rings connected to carbon at...

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Abstract

The invention provides a method for extracting beta-chitin from squid cartilage, and belongs to the field of biological materials. The method includes the steps conducting the protein removal processon the squid cartilage through soda cooking and ultrasonic waves, conducting the calcium removal process on the protein-removed squid cartilage through acid leaching, and further providing concentrated alkaline and a pore-forming agent to conduct the soluble processing process on substances obtained after calcium removal, wherein the finished soluble beta-chitin product processed through soluble processing has the deacetylation degree of 92-96% and the hydrogen content of 6-10%. The method is low in agent consumption, high in work efficiency, short in consumed time and low in cost; product degradation and product color burning can be avoided, and the saturation moisture absorption rate and moisture absorption performance of the product are remarkably increased; the product is high in deacetylation degree and purity, the amino group retention number can be increased so as to increase the molecular hydrogen content of the product, and the crystallinity of the product is remarkably reduced so as to improve the solubility property of the product.

Description

technical field [0001] The invention belongs to the field of biological materials, in particular to a method for extracting beta-chitin from squid cartilage. Background technique [0002] Chitin is a natural polymer linear polysaccharide linked by N-acetyl-D-glucosamine through β-1,4 glycosidic bonds. It is currently the only natural edible animal containing positively charged ionic groups. Fiber is also the only alkaline polysaccharide among sugars. In nature, chitin widely exists in the cells of lower plant fungi and algae, the shells of crustaceans such as shrimp, crab, and insects, and the inner shells of molluscs (such as squid and cuttlefish) in the cell walls of higher plants. Cellulose is the second largest natural polysaccharide. When the chitin that enters the human body is decomposed into basic units, it is the composition of the human body. The basic unit of chitosamine is glucosamine, which exists in the human body. The basic unit of chitin is acetylglucosamin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/08
CPCC08B37/0003C08B37/003
Inventor 郭健蔡璐宋文东纪丽丽王亚宁陈瑛娜
Owner ZHEJIANG OCEAN UNIV
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