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Method for promoting mammalian filial generation growth through maternal addition of phytosterol ester

A phytosterol ester and mammalian technology, which is applied in the field of adding phytosterol esters of maternal origin to promote the growth of mammal progeny, and achieves the effects of regulating the metabolism of bile acid between mother and fetus, the method is simple, and the value of wide popularization and application is achieved.

Pending Publication Date: 2019-12-06
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But, at present, also do not have any relevant phytosterol ester as the raw material of preparation mammalian feed additive, to promote offspring growth and muscle development aspect report

Method used

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  • Method for promoting mammalian filial generation growth through maternal addition of phytosterol ester
  • Method for promoting mammalian filial generation growth through maternal addition of phytosterol ester
  • Method for promoting mammalian filial generation growth through maternal addition of phytosterol ester

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1 Effect of phytosterol ester on bile acid metabolism between mother and fetus

[0046] 1. Preparation of the experimental diet

[0047] (1) Take phytosterol esters (40% β-sitosterol esters, 30% stigmasterol esters and 10% campesterol esters) to prepare a fat-free diet;

[0048] (2) The complete feed was prepared by mixing the phytosterol esters with the fat-free diet, wherein the mass fraction of the phytosterol esters was 3.33% of the complete feed.

[0049] Among them, the formula, nutrient level and preparation method of the fat-free diet refer to the routine, and the equipment for mixing the phytosterol ester and the fat-free diet is conventional equipment.

[0050] 2. Grouping and handling of test mice

[0051] In the experiment, 12 C57BL female mice aged 8-10 weeks in good body condition were randomly divided into 2 groups, 6 mice in each group, and fed with normal diet before pregnancy. At 20 o'clock every day, the male mice and the female mice were mat...

Embodiment 2

[0062] The influence of embodiment 2 phytosterol esters on progeny growth

[0063] 1. Test method

[0064] After the offspring mice were born, the body weight of the offspring mice was measured every week until the age of 8 weeks. After weaning at the age of 3 weeks, the male and female were reared in separate cages and fed with ordinary rations.

[0065] Body composition was determined after the offspring mice grew to 8 weeks of age. After the offspring mice were secured, their tails were stimulated to make them urinate and defecate, and then their body weight was measured and recorded. The offspring mice were gently fixed and put into an MRI instrument (Shanghai Numai Technology Co., Ltd., MesoQMR23-060H type), the muscle and fat content of offspring mice were detected.

[0066] After the determination of the body composition of the 8-week-old offspring mice was completed, the offspring mice were recovered for three days, and the grip strength was measured. Place the off...

Embodiment 3

[0071] The influence of embodiment 3 phytosterol esters on offspring muscle development

[0072] 1. Test method

[0073] Slice the hindlimb buds of 1-day-old offspring mice and the gastrocnemius muscle of 3-week-old offspring mice, fix the slices in 4% paraformaldehyde for 10-20 minutes, wash in water for 1-2 seconds, and stain in hematoxylin for 20-30 seconds , then washed with running water for 5-10s, placed in 1% hydrochloric acid ethanol for 1-3s, rinsed with running water for 15-30s, placed in 1% eosin for dyeing for 30-60s, washed with water for 1-2s, 80% ethanol for 1-2s, 95 % ethanol 1-2s, absolute ethanol 1-2s, xylene (I) 2-3s, xylene (II) 2-3s, neutral gum for sealing. After the H&E stained sections were photographed under a microscope, the number of soleus muscle fibers (Soleus) and the cross-sectional area of ​​gastrocnemius muscle fibers in offspring mice were counted using Image J software for subsequent data analysis.

[0074] The effect of phytosterol ester o...

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Abstract

The invention discloses application of phytosterol ester in preparation of a mammal feed additive, and a method for promoting mammalian filial generation growth through maternal addition of phytosterol ester. According to the invention, the phytosterol ester replaces grease and is added into grease-free daily ration feed for mammals, so that the bile acid level in serum and amniotic fluid, the expression level of a placenta bile acid transporter and the expression level of an embryo posterior limb bud bile acid receptor can be reduced, and the weight, muscle content, gripping power, flatfish muscle fiber quantity, gastrocnemius muscle fiber cross sectional area and myosin heavy chain mRNA expression level can be improved. Besides, after entering the body of the mammal, the phytosterol ester is converted into phytosterol and fatty acid through the digestion effect of the small intestines, no toxic or side effect is generated, and therefore the phytosterol ester has wide application andpopularization value in the aspect of preparing mammal feed additives.

Description

technical field [0001] The invention belongs to the technical field of food and feed. More specifically, it relates to a method of maternally adding phytosterol esters to promote the growth of mammalian offspring. Background technique [0002] In recent years, a large number of studies have confirmed that the nutritional level of the mother during pregnancy plays an important role in the growth, development, immunity and metabolism of the tissues and organs of the fetus and newborn, which marks an important progress in the theory of maternal programmatic regulation of fetal growth and development. Thus, both maternal undernutrition and overnutrition can have adverse effects on the developing fetus. [0003] Bile acids are important regulators of lipid metabolism in animals. Primary bile acids are synthesized from cholesterol in the liver, conjugated with glycine or taurine to increase their solubility, secreted into bile, and converted into secondary bile acids in the colo...

Claims

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Application Information

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IPC IPC(8): A23K20/158A61K31/575A61P21/00
CPCA23K20/158A61K31/575A61P21/00
Inventor 王丽娜赵伟杰左旭冬江青艳王松波束刚朱晓彤高萍
Owner SOUTH CHINA AGRI UNIV
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