A kind of erosion-resistant osteoinductive silk fibroin/hydroxyapatite/magnesia gel sponge and its preparation method

Abstract

The invention discloses a corrosion-resistant silk fibroin / hydroxyapatite / magnesia composite osteoinductive gel sponge and a preparation method thereof. The present invention uses soluble silk fibroin as a stabilizer to prepare nano-suspension with highly dispersed hydroxyapatite and osteoinductive magnesium oxide, and then embeds insoluble silk fibroin fibers to form a three-dimensional network gel, and uses freeze-drying technology to prepare silk Egg white / hydroxyapatite / magnesia gel sponge. After implantation in the gel sponge, it can absorb body fluid and shape it to stay in the injured part, and can maintain the gel state for a long time without being dissolved and lost; under the action of body fluid, the magnesium oxide in the gel sponge slowly degrades to produce magnesium ions and Generate local alkaline microenvironment, promote osteogenic differentiation of bone marrow mesenchymal stem cells, and have osteoinductive effect; the gel sponge can be directly filled in bone defects clinically, without inflammatory reaction and heterotopic ossification; the gel sponge The preparation method is simple.

Description

technical field [0001] The invention belongs to the field of biological composite materials, in particular to the technical field of preparation of medical materials for bone defect repair, and specifically refers to a corrosion-resistant osteoinductive silk fibroin / hydroxyapatite / magnesia gel sponge and a preparation method thereof. Background technique [0002] Bone graft repair materials are the key to the realization of bone trauma treatment, mainly including autologous bone, xenograft bone, and artificial biomaterials. Autologous bone has good osteoconduction, osteoinduction, and osseointegration, and is generally regarded as the gold standard for bone trauma repair. However, due to the limited selection of the body's bone harvesting area and the second trauma caused by the bone harvesting area, the clinical application of autologous bone grafting is limited. Although the source of xenogeneic bone derived from animals or corpses is far more than that of autologous bone...

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Problems solved by technology

Lin et al. used PLGA microspheres to encapsulate magnesium oxide as Mg 2+ Sustained-release reservoir, microspheres coated with alginic acid, preparation of sponge-like gel biomaterials, precise control of Mg 2+ At a concentration of 50ppm, it can effectively enhance the activity of osteoblasts and stimulate the regeneration of rat femoral defects (Biomaterials 174 (2018) 1-16), but the sponge-like gel has rapid water absorption and erosion, and the microspheres dispersed in it are easy to Churn defects

Chinese patent (publication number: CN108939163 A) discloses a magnesium-doped hydroxyapatite silk fibroin composite film with bone-promoting function. Ions and magnesium ions are deposited on the surface of the silk fibroin film, and the prepared composite film has the disadvantages of poor water absorption and slow degradation, and the in vivo application cannot produce a local alkaline microenvironment.

However, due to the high crystallinity and brittleness of hydroxyapatite, and the slow degradation in the body, it is difficult to be completely replaced and utilized by the body, so its clinical application is limited.

Chinese patent (publication number CN103432629A) prepares a kind of silk fibroin / hydroxyapatite composite material by chemical precipitation method through inorganic calcium ion, phosphate ion etc., although this material can improve the mechanical strength and porosity of HA, but this The material has defects such as poor water absorption capacity and weak ability to induce osteoblast differentiation in vivo

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