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High-elasticity water-stable protein-based/epoxy composite superfine fiber tissue engineering scaffold and preparation method thereof

A technology of tissue engineering scaffolds and ultrafine fibers, which can be applied in tissue regeneration, fiber chemical characteristics, cellulose/protein conjugated artificial filaments, etc., can solve the problems of low efficiency and high toxicity, and achieve good growth and high water stability performance, simple post-processing

Active Publication Date: 2019-12-31
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The technical problem to be solved by the present invention is to provide a preparation technology for a biocompatible protein ultrafine fiber scaffold with high elasticity and high water stability, to overcome the defects of low efficiency and high toxicity of the existing modification technology, and to realize the high efficiency of the scaffold. Elasticity, water stability, high mechanical properties, biocompatibility

Method used

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  • High-elasticity water-stable protein-based/epoxy composite superfine fiber tissue engineering scaffold and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A preparation method of a protein-based / epoxy composite superfine fiber tissue engineering scaffold:

[0031] Zein and ethylene glycol diglycidyl ether were added into 75% ethanol while stirring at a ratio of 10:3, and the total mass concentration of zein and ethylene glycol diglycidyl ether was 30%. Add polyvinylpyrrolidone into the spinning solution, the ratio is 5% of the zein mass, then stir at 500rpm at room temperature for 30min until it is completely dissolved, and stand at room temperature for 24h to obtain a zein / ethylene glycol diglycidyl ether solution .

[0032]The spinning solution was placed in the solution chamber of a single-needle electrospinning device, the spinning environment was controlled at 25°C, 40% RH, the fiber receiving device was a drum-type receiver, and the rotating speed was 300r / min. The distance from the spinneret is 18cm, the solution injection speed is determined to be 3.0mL / h during the spinning process, and the voltage is maintained...

Embodiment 2

[0035] A preparation method of a protein-based / epoxy composite superfine fiber tissue engineering scaffold:

[0036] Add wheat protein and diethylene glycol diglycidyl ether into 75% acetic acid while stirring at a ratio of 10:3, and the total mass concentration of wheat protein and diethylene glycol diglycidyl ether is 20%. Add sodium dodecylsulfonate and trimethylolpropane into the spinning solution, the proportions are respectively 5% and 1% of the wheat protein mass, then stir at 500rpm at room temperature for 30min until completely dissolved, and stand at room temperature for 24h to obtain wheat protein / diethylene glycol diglycidyl ether solution.

[0037] The spinning solution was placed in the solution chamber of a single-needle electrospinning device, the spinning environment was controlled at 25°C, 50% RH, the fiber receiving device was a drum-type receiver, and the rotating speed was 300r / min. The distance from the spinneret is 16cm, the solution injection speed is ...

Embodiment 3

[0040] A preparation method of a protein-based / epoxy composite superfine fiber tissue engineering scaffold:

[0041] Collagen and 1,4-butanediol diglycidyl ether were added into 75% ethanol while stirring in a ratio of 10:2, and the total mass concentration of collagen and 1,4-butanediol diglycidyl ether was 25%. Add polyvinylpyrrolidone and glycerin to the spinning solution, the proportions are 5% and 1% of the collagen mass, then stir at 500rpm at room temperature for 30min until they are completely dissolved, and stand at room temperature for 32h to obtain collagen / 1,4-butane Alcohol diglycidyl ether solution.

[0042] The spinning solution was placed in the solution chamber of a single-needle electrospinning device, the spinning environment was controlled at 25°C, 50% RH, the fiber receiving device was a drum-type receiver, and the rotating speed was 300r / min. The distance from the spinneret is 16cm, the solution injection speed is determined to be 3.0mL / h during the spi...

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Abstract

The invention discloses a preparation method of a high-elasticity water-stable protein-based / epoxy composite superfine fiber tissue engineering scaffold. The preparation method comprises: preparing aspinning solution, wherein the components of the spinning solution include a protein, an epoxy compound, and a dispersant, and a chain extender according to requirements; electrospinning the spinningsolution into a scaffold, and then subjecting the scaffold to a stabilizing and strengthening post-treatment and a capping post-treatment in sequence to obtain the high-elasticity water-stable protein-based / epoxy composite superfine fiber tissue engineering scaffold. The scaffold comprises micro-nano fibers and a pore structure, wherein the pore structure is formed by disorderly piling the micro-nano fibers and has a porosity of 50-70% and an average pore size of 1-5 [mu]m. The preparation method provides morphological stability, elasticity and elastic recovery for a protein ultrafine fiber liquid phase, and effectively improves mechanical properties of protein ultrafine fibers. The scaffold widens the application field of the protein-based superfine fiber materials, promotes development of composite functional tissue repair materials, and can realize continuous production.

Description

technical field [0001] The invention belongs to the field of biomedical textile materials and its preparation, and specifically relates to a tissue engineering biological material and its preparation, in particular to a highly elastic and water-stable protein-based / epoxy composite superfine fiber tissue engineering scaffold and its preparation method. Background technique [0002] Electrospinning technology can quickly and stably prepare ultrafine fibers with sizes ranging from tens of nanometers to several microns, and the selection of raw materials is also wide. The prepared ultrafine fiber system has ultra-high specific specific surface area and porosity, and the structure is similar to the three-dimensional (3D) fiber network in natural extracellular matrices (ECMs), which can promote cell attachment, migration, proliferation, and differentiation It also has efficient drug adsorption and delivery functions. The interconnected pore structure in the structure facilitates ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/22A61L27/24A61L27/56A61L27/58A61L27/50D01F8/02D01F8/16D01F1/10
CPCA61L27/227A61L27/24A61L27/50A61L27/56A61L27/58A61L2430/06A61L2430/10A61L2430/24D01F1/10D01F8/02D01F8/16
Inventor 蒋秋冉刘璐李冉刘飞江建财杨圆
Owner DONGHUA UNIV
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