Preparation method and application of selaginella fermentation extract

A technology for extracting liquid and Selaginella, which is applied in the field of probiotic fermentation of Selaginella to prepare extract and high activity lysate, can solve the problem of limited nutrients in Selaginella, achieve enhanced moisturizing, achieve green and environmental protection, good low immunity original effect

Active Publication Date: 2020-05-12
SHANGHAI INSTITUTE OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the research on extracting Selaginella nutrients through fermentation is still relatively limited. Based on the increasing

Method used

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  • Preparation method and application of selaginella fermentation extract
  • Preparation method and application of selaginella fermentation extract
  • Preparation method and application of selaginella fermentation extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A preparation method of Selaginella fermented extract:

[0030] 1) Activation of fermentation strains

[0031] Weigh 0.6g of sodium acetate, 4g of soybean peptone, 4g of glucose, 1g of yeast extract, 0.4g of dipotassium hydrogen phosphate, 0.12g of magnesium sulfate, 0.05g of manganese sulfate, and 200ml of distilled water. The bacteria were cooled to room temperature after 15 minutes, and then inserted into the preserved strain Lactobacillus Brucella CGMCC No.19125 for activation and culture for 48 hours to obtain an activated seed medium.

[0032] 2) Preparation of fermentation medium

[0033] Take the whole plant of Selaginella after drying and dehydration, wash and dry, collect the dried Selaginella leaves and pulverize to obtain 60-mesh Selaginella powder. Add 0.1wt% Selaginella powder, 0.1wt% glucose, 0.001wt% casein, 0.001wt% vitamin C to the fermentation system, make up the rest with water, shake well and then sterilize at 121° C. for 15 minutes.

[0034] 3) ...

Embodiment 2

[0039] A preparation method of Selaginella fermented extract:

[0040] 1) Activation of fermentation strains

[0041] Weigh 0.6g of sodium acetate, 4g of soybean peptone, 4g of glucose, 1g of yeast extract, 0.4g of dipotassium hydrogen phosphate, 0.12g of magnesium sulfate, 0.05g of manganese sulfate, and 200ml of distilled water. The bacteria were cooled to room temperature after 15 minutes, and then inserted into the preserved strain Lactobacillus Brucella CGMCC No.19125 for activation and culture for 48 hours to obtain an activated seed medium.

[0042] 2) Preparation of fermentation medium

[0043]Take the whole plant of Selaginella after drying and dehydration, wash and dry, collect the dried leaves of Selaginella and pulverize to obtain 80 mesh Selaginella powder. Add 1wt% Selaginella powder, 1wt% glucose, 0.02wt% casein, 0.005wt% vitamin C to the fermentation system, make up the rest with water, shake well and then sterilize at 121° C. for 15 minutes.

[0044] 3) Fer...

Embodiment 3

[0049] A preparation method of Selaginella fermented extract:

[0050] 1) Activation of fermentation strains

[0051] Weigh 0.6g of sodium acetate, 4g of soybean peptone, 4g of glucose, 1g of yeast extract, 0.4g of dipotassium hydrogen phosphate, 0.12g of magnesium sulfate, 0.05g of manganese sulfate, and 200ml of distilled water. The bacteria were cooled to room temperature after 15 minutes, and then inserted into the preserved strain Lactobacillus Brucella CGMCC No.19125 for activation and culture for 48 hours to obtain an activated seed medium.

[0052] 2) Preparation of fermentation medium

[0053] Take the whole plant of Selaginella after drying and dehydration, wash and dry, collect the dried leaves of Selaginella and pulverize to obtain 100 mesh Selaginella powder. Add 3wt% Selaginella powder, 2wt% glucose, 0.04wt% casein, 0.01wt% vitamin C to the fermentation system, make up the rest with water, shake well and then sterilize at 121° C. for 15 minutes.

[0054] 3) Fe...

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Abstract

The invention discloses a selaginella fermentation extract, a preparation method thereof, and an application thereof in skin care cosmetics. The raw materials of the selaginella fermentation extract comprise a fermentation system composed of selaginella, glucose, casein, vitamin C and water, and a seed bacterial liquid, wherein the seed bacterial liquid comprises sodium acetate, soybean peptone, glucose, yeast extract, dipotassium hydrogen phosphate, magnesium sulfate, manganese sulfate, distilled water and lactobacillus buchneri. The preparation method is as follows: separately preparing theseed bacterial liquid and the fermentation system; diluting the seed bacterial liquid, then performing inoculation into the fermentation system, and performing standing fermentation; and filtering anobtained fermentation material, separating a liquid phase part from a solid phase part, conducting micro-filtering on the liquid phase part to remove the bacteria, then adding a polyhydric alcohol, and adjusting the pH value to obtain the selaginella fermentation extract. The method uses the natural plant raw materials, probiotic fermentation is supplemented, the degrading effect of the bacterialmetabolic process to macromolecular compounds is fully utilized to prepare the extract with effects such as supplementing water and moisturizing, repairing the basement, and regulating skin micro-ecology.

Description

technical field [0001] The invention relates to a method for extracting highly active cell lysates from Selaginella by fermentation, in particular to a method for preparing an extract by fermenting Selaginella with probiotics, and belongs to the field of biotechnology. Background technique [0002] Selaginella is a native or stone recovery plant, the whole grass is cushion-shaped, and the content of trehalose is about 0.14%, so it has a strong ability to tolerate drought. It can survive under the physiological conditions of dehydration of more than 95% and recover quickly when it encounters water. The content of flavonoids in the whole plant is about 0.42%, so it has the effects of hemostasis, convergence, anti-inflammation and skin repair. And the dry leaves of Selaginella are rich in polysaccharides, amino acids, proteins, and flavonoids, which are natural skin care factors, and have strong moisturizing and antioxidant effects. [0003] At present, most of the patents inv...

Claims

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Application Information

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IPC IPC(8): A61K8/9741A61K8/99A61K8/67A61K8/60A61K8/64A61Q19/00A61Q17/00
CPCA61K8/60A61K8/64A61K8/676A61K8/99A61K2800/85A61Q17/005A61Q19/00A61Q19/005A61K8/9741
Inventor 荣绍丰黄煜玲管世敏李茜茜蔡宝国
Owner SHANGHAI INSTITUTE OF TECHNOLOGY
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