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Rosa graciliflora callus cell extract and application thereof in preparation of external preparation for skin

A skin external preparation and extract technology, applied in the field of plant extract application, can solve problems such as poor solubility, and achieve the effects of stable quality, short culture period and environmental friendliness

Active Publication Date: 2020-05-29
JALA GROUP CORPORATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] mentioned in the patent to inhibit tyrosinase activity is ethanol, butanol and ethyl acetate chromatography and compound 3,5-di-O-methyl - Gamboside, there is no data on the inhibitory activity of the water layer, so the applicant speculates based on the technical knowledge that this compound has poor solubility in water

Method used

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  • Rosa graciliflora callus cell extract and application thereof in preparation of external preparation for skin
  • Rosa graciliflora callus cell extract and application thereof in preparation of external preparation for skin
  • Rosa graciliflora callus cell extract and application thereof in preparation of external preparation for skin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment one, the cultivation of the callus of rose slender stem

[0030] 1. Solid culture

[0031] Take reagent MS medium, 2,4-dichlorophenoxyacetic acid (2,4-D), kinetin (KT), sucrose, agar, glacier water, according to the formula MS +2,4-D3.0 mg / L + KT0.5 mg / L+ sucrose 30 g / L+ agar 6 g / L to prepare the medium, adjust to pH 5.7-5.8, and sterilize in a sterilizing pot at 121°C for 20 minutes for later use. An appropriate amount of callus cells were inoculated in the culture flask. In this embodiment, 0.3-0.5 g of thin-stalked rose callus cells were selected for inoculation according to the capacity of the PC culture flask of 270 ML. The culture conditions are temperature 20±1°C, humidity 60%~75%, light intensity 1500 Lx~2000Lx, 12 hours of light per day, and cells are harvested after 20 days of culture.

[0032] 2. Liquid culture

[0033] Take reagent MS medium, 2,4-dichlorophenoxyacetic acid (2,4-D), kinetin (KT), sucrose, glacier water, according to the formula ...

Embodiment 2

[0035] Embodiment two, the extraction of callus of rose stalk

[0036] 1. Extraction of callus cells from Rosa spp.

[0037] Weigh the thin stem rose callus cells cultivated in Example 1 respectively, weigh 10g each, grind and break, add 5 times the volume of deionized water, immerse and stir, 500W ultrasonic extraction twice, each time for 30min, and centrifuge at 5000rmp for 10min , take the supernatant and filter, combine the filtrates, freeze-dry, after obtaining the extract, weigh and calculate the yield (see Table 1).

[0038] Wherein, the following batches refer to samples collected in different periods under the same experimental conditions.

[0039] Table 1 Extraction yield of callus from Rosa spp.

[0040]

[0041] 2. Determination of polyphenol content in the callus extract of Rosa spp.

[0042] The total polyphenol content was detected by the Folin-Ciocalteu method with gallic acid as the reference substance. Take 0.1 mL of gallic acid standard solution and ...

Embodiment 3

[0045] Embodiment 3, test of extract of callus cell of Rosa spp.

[0046] 1. Effect of callus extract of Rosa spp. on free radical scavenging rate

[0047] 1-Diphenyl-2-trinitrophenylhydrazine (DPPH) is a stable nitrogen-centered organic radical. The DPPH method was proposed in 1958 and is widely used in the quantitative determination of the anti-aging ability of biological samples, classified substances and food. This method is based on the fact that the DPPH radical has a single electron and has a strong absorption at 517nm, and its alcohol solution is purple. When there is a free radical scavenger, its absorption gradually disappears due to pairing with its single electron, and its fading degree has a quantitative relationship with the number of electrons it accepts. Therefore, a spectrophotometer can be used for rapid quantitative analysis to detect free radical scavenging. , so as to evaluate the anti-aging ability of the samples.

[0048] Take 0.1 mL of the extract samp...

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Abstract

The invention relates to application of a rosa graciliflora callus cell extract in preparation of an external preparation for skin. The application is characterized in that the rosa graciliflora callus cell extract serves as an anti-aging active ingredient and a whitening active ingredient in the external preparation for skin. The application has the advantages that discovered by experiments, therosa graciliflora callus cell extract plays roles in clearing skin free radicals from skin cells, increasing propagation of the skin cells, protecting the skin cells from oxidated injury, protecting the skin cells from UVA caused injury and inhibiting activity of tyrosinase of human-derived skin melanocytes B16 and the amount of generation of melanin; and by using rosa graciliflora callus cells asa raw material, the external preparation for skin has the advantages that propagation is infinite, the quality of all batches is stable, the cycle of culture is short, and the external preparation for skin is environmentally friendly and is independent of seasons and easy in batch-size culture, production manufacturing can be carried out in case of not destroying plants, and the external preparation for skin has tremendous value and significance in sustainable development and utilization of plant resources.

Description

[0001] The invention relates to the field of application of plant extracts, in particular to an extract of callus cells from rose slender stems and its application in the preparation of external skin preparations. Background technique [0002] There are many kinds of Rosa plants, which can not only be introduced in gardens as ornamental plants, but also as medicinal plants, and their roots, stems, leaves and flowers can be used as medicine. However, due to the deep dormancy characteristics of rose seeds, it is difficult to germinate in the same year, making garden introduction difficult. Therefore, using plant tissue culture cell technology, a large number of plant cells or callus can be cultivated under artificially controllable and repeatable conditions, and can provide humans with enzyme preparations, pharmaceutical products, additives and other related raw materials without occupying land and resources. It overcomes the disadvantages that the plant resources cannot be reas...

Claims

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Application Information

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IPC IPC(8): A61K36/738A61K8/99C12N5/04A61P17/02A61Q19/02A61Q19/08
CPCA61K36/738A61P17/02A61K8/99A61Q19/08A61Q19/02C12N5/0025Y02A50/30
Inventor 李惠玲章漳周雨露黄俊
Owner JALA GROUP CORPORATION
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