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Lactobacillus casei and application thereof to prevention and treatment of nephritis

A technology of Lactobacillus casei and inflammation, applied in the field of Lactobacillus casei and its application in the prevention and treatment of nephritis, can solve problems that have not been discovered, and achieve the effects of reducing kidney cell apoptosis, protecting kidney function, and promoting regeneration and repair

Active Publication Date: 2020-08-18
BEIJING SCITOP BIO TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Lactic acid bacteria that can slow down the process of chronic inflammation of the kidney, promote the repair of kidney damage, and protect kidney function have not been found so far

Method used

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  • Lactobacillus casei and application thereof to prevention and treatment of nephritis
  • Lactobacillus casei and application thereof to prevention and treatment of nephritis
  • Lactobacillus casei and application thereof to prevention and treatment of nephritis

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Isolation and purification of lactobacillus in the sample of embodiment 1

[0043] For the isolation and purification process of Lactobacillus casei in the sample, see figure 1 .

[0044] 1. The Lactobacillus in the sample is isolated from naturally fermented kumiss, draw 2mL of the kumiss into sterilized small test tubes and seal, 1mL is placed in sterilized CaCO 3 powder in a sterile sampling bottle (volume 2mL), tighten the screw cap as a spare sample and store it in a refrigerator at 4°C, and separate the lactic acid bacteria as soon as possible;

[0045]2. When isolating Lactobacillus, take 1mL kumiss milk sample and inoculate it into 10mL sterilized litmus milk medium, place it in a 30-37°C incubator to enrich the bacteria until acidified and solidified, when the color of the medium turns pink It was taken out and streaked and inoculated on the BL agar medium containing 10ppm cycloheximide and 10ppm colistin sulfate with a sterilized inoculation loop;

[0046...

Embodiment 2

[0050] The identification of embodiment 2 Lactobacillus casei (Lactobacillus casei LC-10)

[0051] The isolated and purified Lactobacillus casei (Lactobacillus casei LC-10) was identified by physiological and biochemical characteristics analysis and 16SrDNA sequence analysis, and its microbiological characteristics are shown in Table 1.

[0052] Table 1 Biological properties of Lactobacillus casei LC-10

[0053]

[0054]

Embodiment 3

[0055] The acid resistance of embodiment 3 Lactobacillus casei (Lactobacillus casei LC-10) and its artificial gastrointestinal fluid tolerance analysis .

[0056] Among the 50 strains of Lactobacillus isolated and identified from the kumiss milk sample, after the growth test in the MRS liquid medium with a pH value of 3.5 and the determination of the survival rate in the artificial gastric juice with a pH value of 3.0, an acid-resistant strain was screened. Strong Lactobacillus, Lactobacillus casei LC-10.

[0057] The Lactobacillus casei LC-10 strain has undergone a growth test in MRS liquid medium with a pH value of 3.5 and a survival rate test in artificial gastric juice with a pH value of 3.0. The results are shown in Table 2.

[0058] Table 2 The growth of Lactobacillus casei LC-10 in pH3.5 and pH3.0 artificial gastric juice

[0059]

[0060] Note: The number of bacteria in the table is the average of two experiments.

[0061] The result shows that Lactobacillus c...

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Abstract

The invention discloses lactobacillus casei and an application thereof to prevention and treatment of nephritis. The potential probiotics lactobacillus casei with high survivability in an intestinal environment is screened out through an in-vitro acid resistance test and a cholate tolerance test, the animal experiments prove that the lactobacillus casei has a protective effect on acute renal ischemia perfusion injury, and secretion of inflammatory factors and the fibrosis degree are reduced; the progress of kidney chronic inflammation is slowed down; regeneration and repair of renal tubules are promoted; kidney cell apoptosis caused by renal ischemia perfusion chronic fibrosis is reduced, and the kidney function is protected.

Description

technical field [0001] The application relates to the field of biotechnology, in particular to a kind of Lactobacillus casei and its application in preventing and treating nephritis. Background technique [0002] Nephritis is an immune disease. It is an inflammatory reaction mediated by renal immunity. After different antigenic microorganisms infect the human body, different antibodies are produced and combined into different immune complexes. Pathological damage caused by different parts forms different types of nephritis. Nephritis is the most common type of kidney disease. Its symptoms include fatigue, back pain, gross hematuria, edema, high blood pressure, abnormal renal function, and decreased urine output. At the same time, in the process of kidney surgery, kidney transplantation and extracorporeal shock wave lithotripsy, different degrees of reperfusion injury can occur, which is the most common cause of acute renal failure. Due to the high perfusion characteristics...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/747A23C9/123A23L2/39A23L33/135A61P13/12C12R1/245
CPCA61K35/747A23C9/1234A23L2/39A23L33/135A61P13/12C12R2001/245C12N1/205A23V2400/125Y02A50/30
Inventor 刘晓军马杰张凌宇张建军其木格苏都杨慧娟
Owner BEIJING SCITOP BIO TECH CO LTD