Snake venom prothrombin activator and snake venom prothrombin activator-based fast hemostatic material

A prothrombin, hemostatic material technology, applied in drug delivery, application, medical science and other directions, can solve the problems of inflammation and tissue necrosis, complex preparation process, skin and other soft tissue damage, etc., to prevent secondary bleeding and infection, experimental The conditions require simplicity, the effect of maintaining activity and stability

Active Publication Date: 2020-08-25
BIOLOGY INST OF SHANDONG ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Zeolites hydrate and release heat during hemostasis, which is easy to cause damage to soft tissues such as skin; kaolin cannot be completely removed from the wound after application, which may cause foreign body granuloma or abscess formation; starch has limited hemostatic effect on uncontrolled bleeding; gelatin Chitosan needs the body's coagulation factors to participate in order to stop bleeding, and it is easy to increase wound infection, especially the polluted wound, and has poor adhesion and easy to fall off. It needs to be fixed with silk thread for visceral hemostasis; the hemostatic mechanism of chitosan hemostatic material is chitosan The surface is positively charged, which makes it easy for negatively charged red blood cells to gather on its surface, and can also activate the complement system in the blood, and also has an aggregation effect on platelets, but for extensive bleeding wounds, chitosan has limited hemostatic effect
[0004] The second type of hemostatic material can cause vascular embolism and release toxic substances such as cyanide and formaldehyde during the degradation process, which can induce inflammation and tissue necrosis around the injection site
However, due to its adhesive and procoagulant effects, it is strictly prohibited to be used in blood vessels to prevent the formation of thrombus to block blood vessels
Fibrin glue has poor adhesion and tends to fall off from the wound
At present, the commonly used fibrin glue is composed of freeze-dried powder and solvent. It must be dissolved before use. It is not suitable for emergencies, and it is not convenient for storage and transportation. Moreover, the cost of fib...

Method used

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  • Snake venom prothrombin activator and snake venom prothrombin activator-based fast hemostatic material

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preparation example Construction

[0054] According to the preparation method of snake venom prothrombin activator of the present invention, 70-100 mg of snake venom prothrombin activator can be obtained per 10 g of snake venom freeze-dried powder.

[0055] The present invention also includes a rapid hemostatic material based on snake venom prothrombin activator, which consists of the following raw materials in parts by weight: 0.01-0.5 parts of snake venom prothrombin activator, 5-10 parts of graphene oxide, and 80 parts of polylactic acid ~100 parts, 3~8 parts of modified chitosan, 8~10 parts of calcium chloride, 2~6 parts of nano titanium dioxide and 1~5 parts of polyvinylpyrrolidone;

[0056] Described modified chitosan is prepared according to the following steps:

[0057] Add the dicarboxylic acid into the aqueous sodium chloride solution with a concentration of 0.4-0.6mol / L, start stirring, add 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride and N- Hydroxysuccinimide, stirred and reacted at 0...

Embodiment 1

[0075] A snake venom prothrombin activator, prepared by the following steps:

[0076] ①Dissolve the lyophilized powder of snake venom in 0.05mol / L PBS buffer solution, filter, and separate and purify the obtained filtrate with a gel column, the filler is Sephadex G-200, first elute with the first mobile phase for 5 hours; The two mobile phases were eluted for 3 hours; the eluent was detected by ultraviolet spectroscopy, and the detection wavelength was 280nm, and the part with ultraviolet absorption was collected, concentrated and desalted with an ultrafiltration membrane with a molecular weight of 5000 to obtain a concentrated solution;

[0077] The mass ratio of snake venom freeze-dried powder and the PBS buffer solution of 0.05mol / L is 1: 10;

[0078] ② The concentrated solution obtained in step ① is subjected to gel column chromatography, the filler is DEAE Sepharose FF, and the first mobile phase is used for elution for 5 hours, and then the second mobile phase is used fo...

Embodiment 2

[0083] A snake venom prothrombin activator, prepared by the following steps:

[0084]①Dissolve the snake venom freeze-dried powder in 0.05mol / L PBS buffer solution, filter, and the obtained filtrate is separated and purified with a gel column, the filler is Sephadex G-200, and first eluted with the first mobile phase for 8 hours; The two mobile phases were eluted for 5 hours; the eluent was detected by ultraviolet spectroscopy, and the detection wavelength was 280nm, and the part with ultraviolet absorption was collected, concentrated and desalted with an ultrafiltration membrane with a molecular weight of 5000 to obtain a concentrated solution;

[0085] The mass ratio of snake venom freeze-dried powder and the PBS buffer solution of 0.05mol / L is 1: 20;

[0086] ②The concentrated solution obtained in step ① was subjected to gel column chromatography, and the filler was DEAE Sepharose FF, and the first mobile phase was used for elution for 8 hours, and then the second mobile ph...

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Abstract

The invention discloses a snake venom prothrombin activator and a snake venom prothrombin activator-based fast hemostatic material. The snake venom prothrombin activator with coagulating activity is extracted by adopting a column chromatography; accurate separation is achieved through changing chromatographic conditions and adjusting technical parameters; and an operation can be carried out at room temperature, so that the requirements for experimental conditions are simple and production is easy to amplify. A selected chromatography column can be repeatedly used, so that the snake venom prothrombin activator has the advantages of low cost, high yield and high product purity. The snake venom prothrombin activator-based fast hemostatic material disclosed by the invention can maintain maximum activity and stability of the snake venom prothrombin activator, is safe and free of toxic and side effects, can rapidly stop bleeding, can prevent secondary bleeding and infection, can be applied to accident emergency, surgical hemostasis and hemostasis of wartime traumas, is especially suitable for arterial haemorrhage, can rapidly stop bleeding and does not generate a secondary trauma.

Description

technical field [0001] The invention relates to the technical field of hemostatic materials, in particular to a snake venom prothrombin activator and a rapid hemostatic material based on the snake venom prothrombin activator. Background technique [0002] There are currently three types of hemostatic materials: the first type is to absorb the water in the blood near the wound through the physical or chemical action of the material, so that the coagulation components of the blood at the wound are concentrated and aggregated, thereby accelerating coagulation; The electrostatic interaction between red blood cells or platelets can improve the adhesion and aggregation ability of red blood cells or platelets, increase the viscosity of blood clots and promote blood coagulation, such as zeolite, kaolin, starch, gelatin and alginate, etc.; Strong adhesive force directly seals the wound surface, thereby realizing hemostasis. This type of hemostatic material is mainly synthetic polyme...

Claims

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Application Information

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IPC IPC(8): A61L24/00A61L24/10A61L24/06A61L24/02A61L24/08A61L24/04
CPCA61L24/001A61L24/0015A61L24/02A61L24/046A61L24/06A61L24/08A61L24/108A61L2300/252A61L2300/418A61L2400/04C08L89/00C08L39/06C08L67/04C08L5/08
Inventor 靳梦刘可春王利振段秀英王荣春张姗姗张云
Owner BIOLOGY INST OF SHANDONG ACAD OF SCI
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