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Near-infrared light response nanoparticle and controlled release system

A near-infrared photoresponse and nanoparticle technology, applied in nanotechnology, nanotechnology, nanomedicine, etc., can solve the problems of uncontrolled release, weak anti-tumor effect, uncontrolled release, etc., to achieve controllable particle size, Good photothermal performance and simple preparation method

Active Publication Date: 2020-09-18
GUANGDONG PROV MEDICAL INSTR INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Diallyl trisulfide (DATS), obtained from the decomposition of garlic-derived polysulfides in water, however, poor solubility and uncontrolled release of DATS in aqueous media, and weak antitumor effects limit its use in the In cancer therapy as H 2 Uses of S Donors
The disadvantages of DATS, such as poor water solubility, uncontrolled release, and weak anti-tumor effect, limit its use as H in anti-tumor therapy. 2 The use of S donors, so how to improve the anti-tumor efficacy of DATS is a big challenge

Method used

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  • Near-infrared light response nanoparticle and controlled release system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Example 1 Preparation method of MPDA-ICG / DATS / EGCG@TD nanoparticles

[0061] Synthesis of Mesoporous Polydopamine Nanoparticles (MPDA NPs)

[0062] 0.15 g of dopamine hydrochloride and 0.1 g of poloxamer (F127) were added to a mixed solution (10 mL) containing deionized water and ethanol, and stirred to dissolve. Then, 160 μL of trimethylbenzene (TMB) was added, and ultrasonically dispersed in a water bath (30° C.) for 2 minutes. Subsequently, 375 μL of ammonia solution was added dropwise with stirring. After reacting for 2 hours, the obtained product was obtained by centrifugation at a speed of 11000 rpm / min, and washed four times with ethanol and water. Finally, the product was redispersed in 5 mL of deionized water.

[0063] Preparation of MPDA-ICG / DATS / EGCG@TD

[0064] Under sonication, 6 mg MPDA NP was dispersed in 3 mL methanol, then 3 mL containing 1 mg mL -1 Methanol solutions of ICG, DATS and EGCG. The reaction was mixed gently at 50 °C until the methanol...

Embodiment 2

[0065] The research of embodiment 2 DATS / EGCG optimum proportioning ratio

[0066] Digest and collect 4T1 cells in the logarithmic phase of growth, resuspend in RPMI1640 complete medium containing 10% FBS and 1% double antibody, seed in 96-well plate at a density of 5000 cells per well, and incubate at 37°C, 5%CO 2 Incubate overnight in a cell culture incubator. Subsequently, the medium was discarded and replaced with fresh complete medium containing different DATS / EGCG molar ratios (1:2, 2:1, 4:1, respectively). After 6h, use a power density of 1W cm -2 808nm laser irradiation for 5min. After continuing to cultivate for 48h), the medium in each well was sucked out, and the CCK-8 reagent diluted in advance was added (the volume ratio of CCK-8 reagent to medium was 1:10), and then cultured in the incubator for 2h , and then use a microplate reader to measure the absorbance of each well at a wavelength of 450 nm. Three sets of parallels were made for each sample. Calculate...

Embodiment 3

[0080] Example 3 Physicochemical properties of MPDA-ICG / DATS / EGCG@TD nanoparticles

[0081] Transmission Electron Microscopy (TEM) Imaging Experiments

[0082] The MPDA-ICG / DATS / EGCG@TD nanoparticles prepared in Example 1 were observed under a transmission electron microscope, and the imaging results are shown in the attached image 3 In (A), it can be seen from the figure that the appearance of MPDA nanoparticles is regular, with a compact mesoporous spherical structure and a single particle size distribution.

[0083] Particle size distribution of MPDA measured by dynamic light scattering (DLS)

[0084] The particle size distribution of MPDA is tested by DLS technology, and the statistical results are shown in the attached image 3 In (B), it can be seen from the results that the average hydrodynamic diameter of MPDA nanoparticles is about 198.6 nm, and the particle size distribution is highly uniform. Dynamic light scattering (DLS) measurements were consistent with TEM (...

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Abstract

The invention discloses a near-infrared light response nanoparticle which comprises a nano-carrier, a nano-carrier entrapped substance, a photosensitizer and a near-infrared light response phase change material for packaging, wherein the nano-carrier entrapped substance comprises an H2S donor and an anticancer drug. Hydrophobic DATS, hydrophilic indocyanine green and epigallocatechin gallate are encapsulated into mesoporous polydopamine nanoparticles by utilizing the amphiphilic property of myristyl alcohol, and finally, the MPDA-ICG / DATS / EGCG@TD nanoparticles are obtained. After the nano system is irradiated by near-infrared laser, ICG in the nano system can effectively convert NIR into heat; and the phase change material TD responds to temperature rise and is changed into liquid from solid, so that rapid release of DATS and EGCG is promoted.

Description

technical field [0001] The present invention relates to the technical field of nanometer delivery systems, and more specifically, to a near-infrared light-responsive nanoparticle and a controlled release system. Background technique [0002] Malignant tumors have become a major killer that seriously threatens human life and health. Chemotherapy is the main method in clinical comprehensive cancer treatment, but it still has serious side effects and limited therapeutic effects. In order to combine two or more drugs for the treatment of cancer, researchers have developed a variety of nanocarriers for drug delivery systems, such as polymer micelles, liposomes, metal-organic frameworks, and inorganic nanomaterials. Polydopamine (PDA), as a mussel biomimetic polymer, has attracted much attention in the field of biomedicine because of its good biocompatibility, easy preparation, high NIR photothermal conversion efficiency and easy modification. nanocarriers. However, the drug loa...

Claims

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Application Information

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IPC IPC(8): A61K47/54A61K47/59A61K47/69A61K41/00A61K31/353A61K31/105A61P35/00B82Y5/00B82Y40/00
CPCA61K47/54A61K47/59A61K47/6935A61K41/0052A61K41/0057A61K31/353A61K31/105A61P35/00B82Y5/00B82Y40/00A61K2300/00Y02A50/30
Inventor 周小雁薛巍顾珩刘群峰于珊
Owner GUANGDONG PROV MEDICAL INSTR INST
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