Bacillus amyloliquefaciens and application thereof

A technology of amylolytic spores and bacilli, which is applied in the direction of application, bacteria, and fungicides, and can solve problems such as unstable application effects and single function of bacteria species

Active Publication Date: 2020-11-10
TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in the microbial fertilizer industry, there are still problems ...

Method used

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  • Bacillus amyloliquefaciens and application thereof
  • Bacillus amyloliquefaciens and application thereof
  • Bacillus amyloliquefaciens and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0119] Example 1. Isolation, Identification and Preservation of Bacillus amyloliquefaciens FH1 CGMCC No.17050

[0120] 1. Separation

[0121]1. Add 10g soil sample (collected from the rhizosphere soil of tea trees in Xixinan Town, Huizhou District, Huangshan City, Anhui Province) in 90mL sterile water, vibrate at high speed for 10min, and let stand for 30s to obtain the supernatant; put the supernatant in Heat in boiling water at 100°C for 10 minutes, cool naturally to obtain a soil suspension (at this time, the dilution is recorded as 10 -1 ).

[0122] 2. Take 1mL of soil suspension and add it into a test tube filled with 9mL sterile water and mix well (the dilution is 10 -2 ), then draw 1mL from this test tube and add it to another test tube filled with 9mL sterile water and mix well to make a dilution (the dilution is 10 -3 ).

[0123] 3. Spread the diluted solution evenly on the LB solid medium, and incubate at 30°C for 3 days. Purify the bacterial colonies that can g...

Embodiment 2

[0143] Embodiment 2, the mensuration of Bacillus amyloliquefaciens FH1 nitrogen-fixing ability

[0144] Ashbey solid medium: add mannitol 10.0g, KH to appropriate amount of distilled water 2 PO 4 0.2g, MgSO 4 ·7H 2 O0.2g, NaCl0.2g, CaCO 3 5.0g, CaSO 4 2H 2 O0.1g and 20g of agar powder, adjust the pH value to 7.0-7.5, then dilute to 1.0L with distilled water, and sterilize at 121°C for 15min.

[0145] 1. A single clone of Bacillus amyloliquefaciens FH1 was inoculated into 10 mL of LB liquid medium, and shaken at 30° C. and 180 rpm for 48 hours to obtain a culture solution.

[0146] 2. After completing step 1, take the cultured bacteria liquid, centrifuge at 4°C and 10,000 rpm for 5 minutes, and collect the bacteria.

[0147] 3. After completing step 2, take the bacteria and dilute with sterile water to obtain a concentration of 1×10 8 Individual / mL of Bacillus amyloliquefaciens FH1 bacterial suspension.

[0148] 4. After completing step 3, spot 5 μL of the bacterial s...

Embodiment 3

[0150] Embodiment 3, the determination of Bacillus amyloliquefaciens FH1 potassium decomposing ability

[0151] Potassium solution solid medium: Add 2.0 g of disodium hydrogen phosphate, 0.5 g of magnesium sulfate heptahydrate, 0.005 g of ferric chloride, 0.1 g of calcium carbonate, 5.0 g of sucrose, 1.0 g of potassium feldspar powder and 20 g of agar powder into appropriate amount of distilled water, Adjust the pH value to 7.0-7.5, then dilute to 1.0L with distilled water, and sterilize at 121°C for 15 minutes.

[0152] Spot 5 μL of the bacterial suspension of Bacillus amyloliquefaciens FH1 prepared in step 3 of Example 2 on the potassium-dissolving solid medium, culture at 30°C for 3 days, and then make the following judgment: if Bacillus amyloliquefaciens FH1 can be cultured on the potassium-dissolving solid Bacillus amyloliquefaciens FH1 has the ability to decompose potassium; if Bacillus amyloliquefaciens FH1 cannot grow on the solid medium for decomposing potassium, then...

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Abstract

The invention discloses bacillus amyloliquefaciens and an application thereof. The bacillus amyloliquefaciens is bacillus amyloliquefaciens FH1, and the preservation number of the bacillus amyloliquefaciens in the China General Microbiological Culture Collection Center is CGMCC No.17050. Experiments prove that the Bacillus amyloliquefaciens FH1 has the effects of dissolving potassium, dissolving phosphorus and fixing nitrogen, and can promote the absorption of potassium elements, phosphorus elements and nitrogen elements by plants; siderophore can be generated, and absorption of plants to ironelements can be promoted. ACC deaminase can be generated, and the stress resistance of plants can be enhanced. The yield and the quality of crops can be improved. Fusarium oxysporum, fusarium graminearum and rhizoctonia solani can be inhibited, so that various plant fungal diseases are prevented and treated. The invention has important application value.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a strain of bacillus amyloliquefaciens and its application. Background technique [0002] Soil is the material basis on which people live and is also an extremely precious natural resource. It plays an extremely important role in the sustainable development of agriculture and animal husbandry. my country has a large population and little land, and its land resources are extremely limited. The per capita arable land area is far below the world average. In order to increase crop yield per unit area, chemical fertilizers and pesticides are widely used. Extensive use of chemical fertilizers and pesticides has not only caused serious ecological and environmental problems (such as soil deterioration, water pollution, air pollution, etc.), but also directly endangered human health. Therefore, there is an urgent need to develop environmentally friendly new fertilizers and pesti...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/22A01P3/00A01P21/00C05F11/08C09K17/14C12R1/07
CPCA01N63/00C05F11/08C09K17/14C12N1/20C12N1/205C12R2001/07
Inventor 王敬敬黄志勇徐松赵维杨榕李晴晴赵思崎
Owner TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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