Three-dimensional porous micro-carrier scaffold and method for preparing scaffold by adopting ionic additive

Abstract

The invention discloses a three-dimensional porous micro-carrier scaffold and a method for preparing the scaffold by the aid of ionic additives. The method comprises the following steps of: 1) mixinga biological material with a buffer solution, adding an ionic pore-forming additive, and mixing to obtain a completely co-soluble premix solution; 2) mixing an organic solvent with a nonionic surfactant to obtain an organic phase solution, and pre-cooling the organic phase solution; 3) preparing an emulsion: mixing the premixed solution with a curing agent under a refrigeration condition to obtaina water phase solution; adding the water phase solution into the organic phase solution for emulsification to obtain W / O type emulsion droplets, and then carrying out a low-temperature light-shielding emulsification reaction on the W / O type emulsion droplets to obtain an emulsion; 4) sequentially removing liquid components from the emulsion, filtering out an organic phase by using a filter screen, and screening out liquid microspheres with particle sizes within a required particle size range, and 5) wetting the liquid microspheres with water, then freezing, and drying after freezing to obtaina three-dimensional porous micro-carrier. By the preparation method, the pore diameter of the three-dimensional porous micro-carrier can be adjusted.

Description

technical field [0001] The invention relates to a three-dimensional porous microcarrier bracket and a preparation method thereof by using an ionic additive, belonging to the field of preparation of microcarrier materials. Background technique [0002] The preparation of three-dimensional porous tissue engineering scaffold materials by freeze-drying method is a good way to maintain the biological activity of biological macromolecules in recent years. The introduction of additives in the ice gel preparation process can change the pore size, hardness and other properties of the scaffold material by regulating the ice crystal formation process. Among the existing methods, the use of salt particles to cause pores is one of the schemes to increase the pore size. The main principle is to rely on the salt particles in the saturated salt solution to occupy space and affect the formation of ice crystals, thereby increasing the pore size. However, the most important problem with this ...

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Problems solved by technology

However, the most important problem with this method is that the density of the salt particles is inconsistent with the density of the solution, and they will aggregate at the bottom of the material under the influence of gravity during the crosslinking reaction, resulting in uneven pore size distribution.

Moreover, a large number of particles with a certain volume cannot exist when the concentration of salt ions is low, and too high a concentration of salt ions will inhibit the chemical cross-linking reaction.

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