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Preparation method of dipeptide-bacterial cellulose membrane

A technology of bacterial cellulose membrane and bacterial cellulose, which is applied in the field of preparation of dipeptide-bacterial cellulose membrane, can solve the problems of poor biodegradability, hinder the application of materials, etc., and achieves low cytotoxicity, low cost, and the ability to promote cell differentiation. Effect

Inactive Publication Date: 2021-01-12
NANJING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But there are also some unavoidable problems, such as the relatively poor biodegradability of the human body due to the lack of suitable glycoside hydrolase, which hinders the further application of this material.

Method used

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  • Preparation method of dipeptide-bacterial cellulose membrane
  • Preparation method of dipeptide-bacterial cellulose membrane
  • Preparation method of dipeptide-bacterial cellulose membrane

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0021] The preparation of dialdehyde BC refers to [Jin X, Xiang Z, Liu Q, et al. Polyethyleneimine-Bacterial Cellulose Bioadsorbent for Effective Removal of Copper and LeadIons from Aqueous Solution [J]. Bioresource Technology, 2017: S0960852417313779.].

[0022] The formula of Acetobacter xylinum fermentation medium in the existing laboratory (w / v, g / 100ml): glucose 2.25, sucrose 2.75, peptone 1.00, yeast extract powder 0.75, ammonium sulfate 0.10, potassium dihydrogen phosphate 0.50, magnesium sulfate 0.07 , calcium lactate 0.02, glacial acetic acid 0.15, citric acid 0.06, sodium carboxymethylcellulose 0.04, adjust pH=6.0, inoculate Acetobacterxylinum NUST 4.2 after high temperature sterilization.

[0023] F-12 medium formula: 84% F-12 medium, 15% fetal bovine serum, 1% vmj double antibody / 100F-12 medium.

Embodiment 1

[0025] Preparation of dialdehyde BC:

[0026] Wet BC film (water content 98.5%) was cut into 1cm×1cm strips, placed in 90mL distilled water, added 0.21g sodium periodate, mixed under magnetic stirring at 150r / min, at room temperature and under dark conditions for 2 days. After the reaction, the dialdehyde BC was filtered and washed with distilled water until the pH was neutral. The purified dialdehyde BC is obtained.

[0027] Preparation and purification of dipeptide-bacterial cellulose membrane:

[0028] Take the purified dialdehyde BC, put it in 80mL distilled water, add 0.2g L-glycerin-glutamic dipeptide, 0.21g sodium cyanoborohydride, adjust the pH to 5.8-6.0 with HCl, and stir at room temperature and 150r / min React for 6 hours. The prepared dipeptide-bacterial cellulose membrane was taken out, filtered with distilled water and washed until the pH was neutral.

Embodiment 2

[0030] MTT test experiment:

[0031] Step 1. Spread the hydrogel sheet in a 96-well plate, soak it in F-12 medium for 4 hours, add 200ul to each well, and place it at 37°C, 5% CO 2 in the incubator;

[0032] Step 2, after sucking off the medium with a pipette gun, insert 5000 cells into each well of the wells containing the material and the wells of the parallel group, and put them into the incubator for culture;

[0033] Step 3, take out the corresponding well plate after 24 hours, add 10ul of MTT to each well, and incubate in the incubator;

[0034] Step 4. After 4 hours, use a syringe with a needle to absorb the liquid in the orifice plate, add 150ul DMSO to each well, and put it into a microplate reader to measure the absorbance;

[0035] Step 5: Shake the microplate reader for 30s to fully dissolve the crystal violet, then take the hydrogel sheet out of the well, detect with a wavelength of 490nm, and record the value.

[0036] Step 6, repeat steps 3-5 at 48h and 72h. ...

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Abstract

The invention discloses a preparation method of a dipeptide -bacterial cellulose membrane. The method comprises the following steps: firstly oxidizing hydroxyl on a bacterial cellulose membrane into an aldehyde group by sodium periodate through a periodic acid Schiff reaction to prepare a dialdehyde bacterial cellulose membrane, then adding the dialdehyde bacterial cellulose membrane into a solution of Glycyl-L-glutamine and sodium cyanoborohydride, adjusting the pH value to 5.8-6.0, and carrying out a stirring reaction to obtain the dipeptide- bacterial cellulose membrane. The method disclosed by the invention is simple in preparation and low in cost, and the prepared dipeptide- bacterial cellulose membrane has the characteristics of low cytotoxicity, good biodegradability, good biocompatibility and the like, has the capability of promoting cell differentiation, and can be used as a biomedical material to be applied to the fields of skin care products and the like.

Description

technical field [0001] The invention relates to a preparation method of a dipeptide-bacterial cellulose film, which belongs to the technical field of biomedical materials. Background technique [0002] Bacterial cellulose (BC) is a high-performance microbial synthetic material produced by certain bacteria. Compared with plant cellulose, bacterial cellulose has no lignin and hemicellulose companions, and has higher purity, tensile strength and higher Young's modulus. In addition, it has good biocompatibility and Degradability and so on make it widely used in the fields of biomedicine, tissue engineering, food and textile. Adding inorganic functional materials to bacterial cellulose can broaden its application field. [0003] At present, BC and its composite materials are mostly used in medicine and sewage treatment, for example, in the preparation of wound dressing materials, artificial skin, and in vascular tissue and bone tissue. However, there are also some unavoidable ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J7/12C08J5/18C08L1/02
CPCC08J7/12C08J5/18C08J2301/02
Inventor 孙东平刘梦迪张衡孙汴京杨蕾林建斌陈辰
Owner NANJING UNIV OF SCI & TECH
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