Nano-drug carrier, nano-drug preparation, preparation method and application
A nano-drug carrier and nano-preparation technology, applied in biochemical equipment and methods, drug combination, drug delivery, etc., can solve the problem that the drug loading and encapsulation efficiency of polymer carriers cannot meet clinical needs, and achieve no immunogen Sexuality, reduced number of administrations, and high stability
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0053] The hydrophobic oleic acid is connected to the side chain carboxyl group of branched polyethyleneimine (molecular weight 600Da) through amide reaction to prepare polyethyleneimine-oleic acid coupling (polyethyleneimine-oleic acid) with a modification degree of 10.4 %.
[0054] Dissolve 2mg of silibinin, 5mg of polyethyleneimine-oleic acid and 30mg of Solutol HS15 (stabilizer) in methanol in a round-bottomed flask, rotary evaporate to form a film, add 5% glucose solution for hydration, and obtain polyethylene Imine-oleic acid drug-loaded nano-preparation ( figure 1 , 2 Among them, nanoformulation particles 1). Add polyethyleneimine-oleic acid drug-loaded nano-preparation to dissolve 1mg / mL silibinin-collagenase to obtain figure 1 with 2 Nanoformulation Particles 2. Finally, the nano-preparation particle 2 is added in the 5% glucose solution of 1mg / mL chondroitin sulfate, stirred for 5min to obtain the nano-preparation of silibinin-collagenase (such as figure 1 with...
Embodiment 2
[0057]Hydrophobic stearic acid is connected to side chain carboxyl group of branched polyethyleneimine (molecular weight: 1.8kDa) through amide reaction to prepare polyethyleneimine-stearic acid coupling product with a modification degree of 6.2%. Dissolve 5mg of polyethyleneimine-stearic acid, 35mg of Solutol HS15, 2mg of DSPE-PEG2000 and 1mg of paclitaxel (small molecule drug) in ethanol in a round-bottomed flask, rotary evaporate to form a film, add normal saline to hydrate, and obtain Polyethyleneimine-stearic acid drug-loaded nanoformulations. Among them, Solutol HS15 and DSPE-PEG2000 are stabilizers.
[0058] The polyethylenimine-stearic acid drug-loaded nano-preparation was sequentially added to the solution containing 50 μg / mL CpG (nucleic acid drug), 500 μg / mL ovalbumin (protein drug) and 2 mg / mL folic acid-polyethylene glycol conjugate (Targeting functional molecules) in physiological saline, stirred for 10 minutes to obtain a three-drug-loaded nano-preparation with...
Embodiment 3
[0060] By amide reaction, hexadecanoic acid is connected to the side chain carboxyl group of linear polyethyleneimine (molecular weight is 25kDa), and the polyethyleneimine-hexadecanoic acid coupling product (polyethyleneimine-hexadecanoic acid) is prepared. The degree of modification is 7.1%.
[0061] Dissolve 4 mg polyethyleneimine-hexadecanoic acid, 30 mg poloxamer 188 (stabilizer) and 1 mg doxorubicin (small molecule drug) in methanol and place in a round-bottomed flask, rotary evaporate to form a film, add amino acid The solution is hydrated to obtain the polyethyleneimine-hexadecanoic acid drug-loaded nano-preparation. The polyethyleneimine-hexadecanoic acid drug-loaded nano-preparation was successively added to the phosphate buffer solution (aqueous solvent) dissolved with 1 mg / mL ovalbumin (protein drug) and folate (targeting functional molecule) , and stirred for 5 minutes to obtain a double drug-loaded nano-preparation with a particle size of 50nm.
PUM
Property | Measurement | Unit |
---|---|---|
molecular weight | aaaaa | aaaaa |
particle diameter | aaaaa | aaaaa |
particle size | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com