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A kind of extraction method and application of Korla fragrant pear acid polysaccharide

A technology of Korla fragrant pear and acid polysaccharide, which is applied in the extraction field of Korla fragrant pear acid polysaccharide, can solve problems such as inflammatory reaction, and achieve the effects of good activity, simple operation and remarkable curative effect.

Active Publication Date: 2022-07-15
XINJIANG MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The traditional method of improving the immune regulation activity mediated by macrophages is mainly to intervene with lipopolysaccharide, but long-term and large-scale use of lipopolysaccharide can easily induce inflammatory reactions

Method used

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  • A kind of extraction method and application of Korla fragrant pear acid polysaccharide
  • A kind of extraction method and application of Korla fragrant pear acid polysaccharide
  • A kind of extraction method and application of Korla fragrant pear acid polysaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The extraction method of the Korla fragrant pear acidic polysaccharide of the present embodiment, the method is:

[0034] S1. Cut the core of the Korla fragrant pear fruit into shreds, place it in a cool and ventilated place for 1 month to dry, obtain the dried pulp, and soak the dried pulp in an aqueous ethanol solution with a mass fraction of 95% to degrease After 8 hours, take it out to obtain the degreasing pulp, add distilled water a to the degreasing pulp, then reflux and extract at a temperature of 90 ° C and filter to obtain filtrate a, add distilled water b to the filtered pulp residue a, Under the condition that temperature is 90 ℃, reflux extraction is filtered to obtain filtrate b, distilled water c is added to the pulp residue b after filtration, and the temperature is 90 ℃ under the condition of reflux extraction and filtration to obtain filtrate c, and filtrate a, combined with filtrate a, Filtrate b and filtrate c to obtain extract; reflux extraction tim...

Embodiment 2

[0047] This example provides the application of two kinds of Korla pear acid polysaccharides prepared in Example 1. The Korla pear acid polysaccharides PSAP-1 and PSAP-2 are used to reduce macrophages, neutrophils, The number of eosinophils and lymphocytes, reduce the content of IL-4, IL-5, IL-13, repair the damage of lung tissue.

[0048] 72 normal female BALB / c mice, weighing 18±5g, were randomly divided into 9 groups, 8 mice in each group, normal control group, asthma model group, PSAP-1 low-dose group (10mg / kg), PSAP-1 Medium-dose group (20mg / kg), PSAP-1 high-dose group (40mg / kg), PSAP-2 low-dose group (10mg / kg), PSAP-2 medium-dose group (20mg / kg), PSAP-2 high-dose group group (40mg / kg), dexamethasone positive control group. Except for the normal control group, the other 8 groups were sensitized by intraperitoneal injection of 0.2 mL of antigen solution (containing 100 μg of OVA and 200 μg of aluminum hydroxide dry powder) three times on the 1st, 7th and 14th days. Sensi...

Embodiment 3

[0066] This example provides the application of two kinds of Korla pear acid polysaccharides prepared in Example 1. The Korla pear acid polysaccharides PSAP-1 and PSAP-2 are used to improve the proliferation activity of RAW264.7 macrophages and promote RAW264.7. 7 The release of NO from macrophages promotes the secretion of cytokine IL-6 and cytokine TNF-α from RAW264.7 macrophages, and improves the immunomodulatory activity mediated by macrophages.

[0067] (1) Cell proliferation activity

[0068] Take RAW264.7 macrophages in the logarithmic growth phase to 1 × 10 5 The density of cells / mL was seeded in 96-well plates and cultured in a carbon dioxide incubator for 24h until the cells adhered. After discarding the supernatant, the concentration of PSAP-1 and PSAP-2 (3.9, 7.8, 15.6, 31.3, 62.5, 125, 250, 500, 1000, 2000 μg·mL) -1 ) complete cell culture medium for cell intervention, and a lipopolysaccharide positive control group (LPS at a concentration of 250 μg·mL) was set ...

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Abstract

The invention provides a method for extracting Korla fragrant pear acidic polysaccharide. The method comprises the following steps: removing the core of Korla fragrant pear fruit, shredding, drying, defatting, and refluxing to extract an extract; after evaporating and concentrating, adding ethanol aqueous solution to collect the precipitate , after dissolving with distilled water, through dialysis, concentration and vacuum freeze-drying, the Korla pear polysaccharide is obtained, and after three elutions, two kinds of Korla pear acidic polysaccharides PSAP-1 and PSAP-2 are obtained. The present invention also provides the application of PSAP-1 and PSAP-2 for reducing the number of macrophages, neutrophils, eosinophils and lymphocytes in airway inflammation, reducing IL-4, IL-5, The content of IL-13 repairs the damage of lung tissue. It is used to improve the proliferation activity of RAW264.7 macrophages, promote the release of NO from RAW264.7 macrophages, promote the secretion of cytokines IL-6 and cytokine TNF-α by RAW264.7 macrophages, and improve macrophage-mediated immunomodulatory activity.

Description

technical field [0001] The invention belongs to the technical field of Korla fragrant pear acidic polysaccharide, in particular to a method for extracting Korla fragrant pear acidic polysaccharide and application thereof. Background technique [0002] The extraction methods of plant polysaccharides mainly include acid-base extraction, biological enzyme extraction, ultra-high pressure extraction and microwave extraction. The acid-base extraction method is easy to break the glycosidic bond, the biological enzyme extraction method is costly, the ultra-high pressure extraction method is easy to damage the internal structure of the polysaccharide, and the microwave extraction method has higher requirements on equipment. [0003] The separation methods of plant polysaccharides mainly include: fractional precipitation method, quaternary ammonium salt precipitation method, electrophoresis method and metal ion precipitation method. The fractional precipitation method can only be use...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A61K36/73A61K31/715A61P37/02A61P37/04A61P11/00
CPCC08B37/0003A61K36/73A61K31/715A61P37/02A61P37/04A61P11/00
Inventor 海力茜·陶尔大洪董彩霞周凯马晓丽杨飞陈春丽庞国凤汪芳聂昌宏热孜亚·吾甫尔李亚童李欢欢乔丽洁阿吉然·阿布拉米合热尼沙·阿木热江卡地尔亚·库尔班
Owner XINJIANG MEDICAL UNIV
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