Method for improving gas microenvironment of plant tissue culture bottle seedling and special culture medium

Abstract

The invention discloses a method for improving a gas microenvironment of a plant tissue culture bottle seedling and a special culture medium, and particularly relates to a method for adjusting carbondioxide and moisture in a plant tissue culture bottle. After bud seedlings are inoculated in the culture medium, CO2 slow-release particles are put into the bottle and flatly laid on the surface of the culture medium, a moisture absorption bag is hung at the position close to a bottle opening, and the bottle is capped for culture. The CO2 supply amount in the bottle is increased to facilitate photosynthesis of the bottle seedling, the humidity in the culture bottle is reduced, epidermal cuticles of leaves of the bottle seedling are developed, the stomatal opening and closing function is enhanced, transpiration is reduced, and the transplanting survival rate of the bottle seedling is increased. The use amount of sucrose in the culture medium can be reduced, so that the culture medium pollution rate is reduced. The CO2 slow-release particles, the moisture absorption bag and the application method of the medium have the characteristics of low cost, simplicity in preparation, convenience in application and remarkable effect.

Description

technical field [0001] The invention belongs to the technical field of plant biology and relates to a plant tissue culture method, in particular to a method for regulating carbon dioxide and water in a plant tissue culture bottle and a special culture medium. Background technique [0002] (1) Plant tissue culture technology has broad market prospects for seedling production, virus removal, and germplasm improvement. "In traditional plant tissue culture, tissue culture seedlings are cultivated in airtight test tubes or glass bottles, due to the high humidity in the container, CO 2 Insufficient, so that the cultivated small plants cannot normally carry out photosynthesis, but have to rely on the sugar in the medium for heterotrophic growth, resulting in too long culture time, small plants grow slowly, the plants grow thin, and survive after transplanting The sugar in the medium will also promote the reproduction of microorganisms and increase the pollution rate of plants” [Ma...

AI Technical Summary

Problems solved by technology

"In traditional plant tissue culture, tissue culture seedlings are cultivated in airtight test tubes or glass bottles, due to the high humidity in the container, CO 2 Insufficient, so that the cultivated small plants cannot normally carry out photosynthesis, but have to rely on the sugar in the medium for heterotrophic growth, resulting in too long culture time, small plants grow slowly, the plants grow thin, and survive after transplanting The sugar in the medium will also promote the reproduction of microorganisms and increase the pollution rate of plants” [Ma Mingjian, Song Yuedong. A sugar-free culture system for tissue culture seedlings based on environmental control. Journal of Agricultural Engineering, 2009, 25 (6) :192-197], specifically as follows

[0003] 1. At present, the carbon source sucrose content in the plant tissue culture medium is generally 3%, and the light and dark are alternately cultivated every day during the cultivation. "During the dark period, due to the high concentration of CO in the bottle due to respiration 2 , within 1-2 h of the start of light, CO 2 The concentration is from thousands of μl·L -1 Rapidly drop to 70~90μl·L -1 , and maintained this low concentration state during the subsequent photoperiod” [Guo Hai, et al. CO 2 The application and prospect of gas in plant tissue culture. Acta Nuclear Agriculture, 2004,18(5):368~371], which is close to the average CO of plant photosynthesis 2 Compensation point 50μl·L -1 , than atmospheric CO 2 Concentration 350μl·L -1 Much lower, photosynthetic efficiency is very low

[0004] 2. "The relative humidity in the incubator is close to 100%, so that the epidermal cuticle of the small plants is not developed enough, and the water potential during the domestication period is low, which leads to a decrease in the survival rate of seedlings during the domestication stage

[0006] (2) At present, there are roughly two types of gas microenvironment control systems for tissue culture: open and semi-open, both of which have problems such as special equipment and many links involved, and cannot be widely used [Xu Zhigang, et al. Automatic regulation of gas microenvironment for group culture seedlings System development and test. Journal of Agricultural Engineering, 2003, 19(6):14-17]

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