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Composite guided tissue regeneration membrane and preparation method thereof

A technology that guides tissue regeneration and magnesium alloys, applied in the field of biomedicine, can solve problems such as increasing patient pain and cost, affecting bone tissue regeneration, and affecting mechanical properties, and achieves simple and easy preparation methods, promotion of growth and repair, and easy promotion and apply the effect

Pending Publication Date: 2021-04-20
宁波瑞瑧生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Currently, there are two types of guide tissue membranes used clinically: one is a non-absorbable membrane based on polytetrafluoroethylene. The advantages of this type of membrane are stability, biological inertness, certain mechanical support and good Biocompatibility, however, the disadvantage of non-degradable means that a second operation is required to remove it, which will cause secondary damage to the patient, thereby increasing the pain and cost of the patient; the other is the absorbable film, which is mainly divided into biodegradable high Molecular film and animal-derived collagen film, this material can be degraded and absorbed by the human body, and the degradation products are water and carbon dioxide, which can be excreted through metabolism. Patients do not need secondary operations, and the absorbable film has good biological activity and can be regenerated Good at promoting bone tissue regeneration, but this kind of material usually degrades quickly, about 2-6 weeks, and with the continuous degradation of the membrane, its mechanical properties are affected, it collapses in the body, and the space maintains failure, thus affecting bone tissue regeneration

Method used

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  • Composite guided tissue regeneration membrane and preparation method thereof
  • Composite guided tissue regeneration membrane and preparation method thereof

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preparation example Construction

[0051] The invention provides a method for preparing a composite guided tissue regeneration membrane, which comprises:

[0052] The upper matrix layer, the magnesium alloy film and the lower matrix layer are arranged sequentially from top to bottom, and then a composite guided tissue regeneration membrane is prepared by freeze-drying-vacuum lamination.

[0053] In a preferred embodiment of the application of the present invention, the vacuum degree is 0.05-0.5 Pa and the temperature is -40--10°C when the above-mentioned vacuum drying is carried out by the freeze-drying-vacuum lamination method. Optionally, the vacuum degree during vacuum drying is 0.1-0.5Pa, and the temperature is -30--20°C; optionally, the vacuum degree during vacuum drying is 0.1-0.2Pa, and the temperature is -20°C.

[0054] In one embodiment, the vacuum drying time is 20-50 hours. Optionally, the vacuum drying time is 24-48h.

[0055] In one embodiment, after vacuum drying, the dried film is vacuum compac...

Embodiment 1

[0080] This embodiment provides a composite guiding tissue regeneration membrane and a preparation method thereof. The composite guiding tissue regeneration membrane is composed of an upper matrix layer, a magnesium alloy membrane and a lower matrix layer arranged in sequence from top to bottom.

[0081] First, the acellular small intestinal submucosa matrix material is prepared, and in other embodiments, a commercially available acellular small intestinal submucosa matrix material can also be selected. The preparation method of the decellularized small intestine submucosa matrix material in this example comprises the following steps:

[0082] 1) Obtain fresh Changbai pig jejunum from the slaughterhouse, rinse out the contents, and freeze and preserve;

[0083] 2) Mechanically peel off the mucous membrane layer, muscular layer and serosa layer on the jejunum, and rinse it;

[0084] 3) Using 0.5% peracetic acid solution to sterilize the small intestine;

[0085] 4) Using eth...

experiment example 1

[0095] This experimental example refers to the standard of "YY / T 0606.25-2014 Tissue Engineering Medical Products Part 25 Determination of DNA Residues in Animal-derived Biomaterials: Fluorescent Staining Method". Quantitative detection was carried out, and the results were as follows figure 1 As shown, the DNA content in SIS before decellularization was 835.73±9.65ng / mg, and the DNA content after decellularization (dSIS) was 52.50±1.23ng / mg.

[0096] The results show that the decellularization process can obviously remove the immune antigenic substances in SIS, thereby improving the biosafety performance of SIS.

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Abstract

The invention discloses a composite guided tissue regeneration membrane and a preparation method thereof, and relates to the technical field of biomedicine. The composite guided tissue regeneration membrane comprises an upper matrix layer, a magnesium alloy membrane and a lower matrix layer which are sequentially arranged from top to bottom, wherein the upper matrix layer and the lower matrix layer are both composed of active ingredients, namely acellular small intestine submucosa matrix materials. The membrane has excellent mechanical properties, can provide enough growth space for bone tissue regeneration, has excellent biological activity, osteoinductivity and degradability, can be maintained in the human body for 8-12 weeks, and provides enough space maintenance time for bone tissue growth, thereby promoting the growth and repair of bone tissue.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a composite guiding tissue regeneration membrane and a preparation method thereof. Background technique [0002] Periodontitis is a serious oral disease caused by dental plaque, and it has a high incidence rate. Periodontitis is also the main cause of tooth loss in adults. At the same time, periodontitis will also affect the functions of important organs such as the heart, lungs, and kidneys. Periodontal bacteria produce a large amount of endotoxin that can enter the blood, which can trigger a series of pathological reactions in vascular endothelial cells, and is a disease that leads to various high mortality rates. important cause. The chances of coronary heart disease and stroke in patients with periodontitis were 1.4 times and 2.1 times of those with normal periodontitis, respectively. Therefore, the clinical treatment of periodontitis is particularly important and urgen...

Claims

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Application Information

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IPC IPC(8): A61L31/12A61L31/14A61L31/02A61L31/08A61L31/00C23C26/00C22C23/02
Inventor 邹鹏陈琦谭睿哲梁丽金李锐聪
Owner 宁波瑞瑧生物科技有限公司
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