Unlock instant, AI-driven research and patent intelligence for your innovation.

Yeast strain as well as screening method and application thereof

A screening method and technology of Saccharomyces, applied in the field of Saccharomyces strains and their screening, can solve the problems of low bioavailability, poor fat solubility, etc., achieve the effects of simple and easy preparation, regeneration of coenzyme, and improvement of medicinal value

Active Publication Date: 2021-04-30
HUAIYIN INSTITUTE OF TECHNOLOGY
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, tofu glucoside is a polyhydroxy compound with poor fat solubility and low bioavailability

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Yeast strain as well as screening method and application thereof
  • Yeast strain as well as screening method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach 1

[0023] This embodiment provides a screening method for Saccharomyces species:

[0024] S1: Preparation of selective yeast enrichment medium: in terms of mass volume ratio, the composition of selective yeast enrichment medium is (w / v): 0.01% yeast extract, 0.05% MgSO 4 ·7H 2 O, 1%Na 2 HPO 4 , 0.5% NaCl, 0.2% KH 2 PO 4 , 2% mixed vegetable oil, 0.03% streptomycin, 0.03% cycloheximide. The mixed vegetable oil is composed of walnut oil, camellia oil and olive oil in a volume ratio of 1:1:8. Its mixing ratio is 1:1:8).

[0025] Preparation of neutral red fat plate culture medium with a pH of 7.0 to 7.5: in terms of mass volume ratio, the composition of neutral red fat plate culture medium is (w / v): 0.3% peptone, 0.3% beef extract, 10%-15 % mixed vegetable oil polyvinyl alcohol emulsion, 0.75% NaCl, 0.05% MgSO 4 ·7H 2 O, 0.2% agar powder, 0.03% chloramphenicol, 0.03% streptomycin, 0.02% neutral red solution. The above-mentioned mixed vegetable oil polyvinyl alcohol emulsio...

Embodiment approach 2

[0041] Embodiment 2: The application experiment of Saccharomyces SA-X as a whole-cell catalyst in the acylation reaction of tofu fruit under non-aqueous phase conditions

[0042] Venue: Biocatalysis Laboratory, School of Biological Sciences, Huaiyin Institute of Technology

[0043] Inoculate the SA-X (CGMCC No.20048) strain into the seed liquid medium at 30°C, shake at 180rpm for 24h, then inoculate it with 2% inoculum in the fermentation medium at 30°C, shake at 180rpm for 48h, then 8000rpm Centrifuge for 10 minutes, discard the supernatant to obtain wet cells, wash twice with deionized water, pre-freeze overnight at -80°C, and freeze-dry in vacuum at -45°C for 30 hours. The obtained freeze-dried cells are SA-X whole-cell catalysts . Store in a sealed refrigerator at 4°C for later use. After optimizing the culture conditions, the biomass of whole-cell catalyst of SA-X strain can reach 1.9g / L.

[0044] Acylation reaction experiment: take tofu glucoside, strain SA-X whole-ce...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the field of whole-cell catalysis, and discloses a yeast strain as well as a screening method and application thereof. The yeast strain is preserved in China General Microbiological Culture Collection Center (CGMCC) on June 8, 2020, the preservation address is Institute of Microbiology, Chinese Academy of Sciences, No.3, No.1 Yard, West Beichen Road, Chaoyang District, Beijing, and the strain preservation number is CGMCC No.20048 SA-X strain whole cells can catalyze the acylation reaction of helicid in a non-aqueous phase system. The yeast strain is used as a whole-cell catalyst under a non-aqueous phase condition, and the catalytic activity of the acylation reaction of the helicid can be remarkably improved.

Description

technical field [0001] The invention relates to the field of microorganisms and biochemical industry, in particular to a strain of Saccharomyces strain capable of catalyzing tofu fruit glycosides to generate ester derivatives in a non-aqueous phase system, and a screening method and application thereof. Background technique [0002] As the main active ingredient in longan fruit, tofu glucoside has been clinically used to treat various diseases, such as neurasthenia syndrome, trigeminal neuralgia and vascular headache. Some recent studies have found that tofu glucoside also has the potential to treat depression and skin diseases related to melanosis. However, tofu glucoside is a polyhydroxy compound with poor fat solubility and low bioavailability. Studies have shown that tofu fruit glycoside derivatives have better biological activity and bioavailability than parent compounds. Cinnamic acid belongs to phenolic acid compounds, widely exists in nature, and has various biolog...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16C12N1/02C12P19/44C12R1/645
CPCC12N1/16C12N1/02C12P19/44
Inventor 杨荣玲许宁宁陈曦赵祥杰仝争宋毓晔王朝宇罗洪镇何婷婷
Owner HUAIYIN INSTITUTE OF TECHNOLOGY