A strain of Lactobacillus fermentum mf423 and its fermented rice bran extract and its application
A technology of fermenting Lactobacillus and rice bran extract, applied in the field of microorganisms, can solve the problems of low utilization rate of rice bran, achieve the effects of reducing glucose content, increasing glucose consumption, and eliminating lipid accumulation
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Embodiment 1
[0061] Example 1 Separation sample processing of Lactobacillus fermentum MF423: draw 500 μL of rice flour fermentation wastewater stored at 4°C, draw 50 μL, 100 μL and 200 μL respectively, and apply them on MRS solid medium plates, and each coating amount is set Three replicates were then placed in a 37°C incubator for static culture. After each colony grows well, the plate is streaked repeatedly, and the strain is separated and purified to finally obtain the strain MF423.
Embodiment 2
[0062] Example 2 Biological traits and physiological, biochemical and molecular identification of strain MF423
[0063] S1. Morphological characteristics: see the morphology of strain MF423 under scanning electron microscope. figure 1 , the strain was cultured on MRS solid medium plate for 24h. The colony morphology was as follows: the colony was white, round, with smooth surface and neat edge; Gram staining was positive, and the cell was rod-shaped.
[0064] S2. Physiological and biochemical characteristics: bacterial strain MF423 of the present invention is measured by physiological and biochemical conditions, and the contact enzyme test is positive, the methyl red test is negative, the V-P test is negative, and the starch hydrolysis, agar hydrolysis, Tween 80 hydrolysis are negative, and the growth temperature is 4-30℃.
[0065] See Table 1.
[0066] Table 1 Physiological and biochemical characteristics of Lactobacillus fermentum MF423
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Embodiment 3 3
[0081] Example 3 In vitro antioxidant activity of three different Lactobacillus fermented rice bran extracts
[0082] The three strains of Lactobacillus were inoculated into MRS liquid medium, cultured at 37°C for 24 hours, respectively inoculated with fermentation medium at 2%, cultured at 37°C, and the fermented broth for 24 hours was taken and centrifuged at 10,000 rpm twice to remove bacteria and cells. Rice bran residue, take the upper fermentation broth to measure hydroxyl radical scavenging activity: add 40 μL Fe to a clean EP tube 2+ , then add 40 μL OP, mix well, the solution turns into an orange-red complex, add to the experimental group (A s ) and the negative control group (A n ) was added with 80 μL of sample, blank control group (A b ) add 80 μL dd H 2 O, after mixing evenly, start the reaction, the experimental group (A s ) add 40 μL H 2 O 2 , the negative control group (A n ) add 40 μL H 2 O, blank control group (A b ) add 40 μL H 2 O 2 . Then put i...
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