Alternaria tenuissima as well as metabolite and application thereof

A technology of extracting Alternaria microbes and extracts, which is applied in the field of functional microorganisms and plant disease prevention and control, and can solve problems such as lack of viral diseases

Active Publication Date: 2021-08-20
SHANDONG AGRICULTURAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the lack of biocontrol bacteria for effective prevention and treatment of viral diseases in the prior art, the present invention provides a strain of Alternaria superfineum (

Method used

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  • Alternaria tenuissima as well as metabolite and application thereof
  • Alternaria tenuissima as well as metabolite and application thereof
  • Alternaria tenuissima as well as metabolite and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Isolation and Identification of Alternaria tennatus SDTF016

[0033] Fungal strains were isolated from farmland soil near Mount Tai collected in Tai'an City. The specific separation and purification process is as follows: weigh 10 g of the sieved soil sample, put it into a 250 mL sterilized Erlenmeyer flask filled with 90 mL of sterile water and a small amount of glass beads, and place the Erlenmeyer flask on a shaker at 37°C and 180 rpm Shake for 30 minutes to make it fully suspended, and dilute the suspension to 10 with sterile water -2 -10 -5 Wait for 5 gradients. Pipette 1 mL of each gradient dilution into a 9 cm petri dish and pour 15 mL of modified Martin medium (containing Bengal red 0.033 g / L) cooled to about 45 °C, shake and mix horizontally, repeat 3 times for each gradient, and set After culturing in a constant temperature incubator at 28°C for 5-7 days, different single colonies were picked and purified by streaking on a plate. The purified str...

Embodiment 2

[0037] Example 2 Obtaining Alternaria tengini SDTF016 Fermentation Broth and Extraction of Fermentation Products

[0038] Using multi-factor orthogonal test to optimize the fermentation conditions of Taishan variety (medium carbon source, nitrogen source, composition and content of inorganic salts, fermentation time 12-120h, fermentation temperature 20-37°C, rotation speed 90-150rpm), the following optimized Conditions for fermentation: Use a hole punch with a diameter of 7 mm to punch the fungus cake at the edge of the PDA plate colony of the Alternaria superficialis SDTF016 strain, and connect it to 150 mL of liquid fungal culture medium (MgSO 4 ·7H 2 O 0.5 g, K 2 HPO 4 1.0 g of peptone, 5.0 g of peptone, 20 g of glucose, 2.0 g of yeast powder, 1.0 L of water, pH=7.0), 3 bacteria cakes were placed in each bottle, and the culture was shaken at 120 rpm at 25°C for 36 hours to obtain very fine chains Bacillus sp. SDTF016 strain fermentation broth, 5 batches of parallel ferme...

Embodiment 3

[0042] Example 3 The preventive effect of Alternaria tennatus SDTF016 fermentation broth and extracts on tomato virus diseases

[0043] Ferment Alternaria ultrafines SDTF016 according to the fermentation method in Example 2 and extract Taishanmycin, and obtain a bacteria-containing fermentation broth (Taishanmycin content is about 0.02%), a bacteria-free fermentation broth (Taishanmycin content is about 0.02%) ) and Taishanmycin, Taishanmycin was prepared with 0.1% TX-100 aqueous solution into an aqueous solution with a content of 0.02wt%. Referring to the method of Diao Hailing, 2018 (Effect and physiological mechanism of metabolites of Alternaria Taishan strain in controlling tomato yellow leaf curl virus), Alternaria Taishan strain TS001 was fermented to obtain fermentation broth. Select the greenhouse where the tomato leaf curl virus takes place seriously to carry out the test. For the spraying control, the above-mentioned fermented liquid or water preparation is diluted 2...

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Abstract

The invention provides an Alternaria tenuissima SDTF016, a fermentation condition and an extraction and separation method of a metabolite Taishan mycin of the Alternaria tenuissima SDTF016 are obtained. The fermentation liquor and metabolite Taishan mycin both promote plant growth, effectively prevent and control virus diseases on crops such as tomatoes, tobaccos, peppers and potatoes, improve the stress resistance and disease resistance of plants and improve the yield and quality. The fermentation liquor of the Alternaria tenuissima and the extract thereof can be diluted by adding water and then used for seed dressing, seed soaking, root irrigation, spraying, mist spraying and drip irrigation, which have high biological activity and broad-spectrum action, have multiple effects of improving the control effect, reducing the pesticide dosage and improving the yield and quality of agricultural products when being mixed with pesticides, and are environment-friendly. The fermentation liquor of the Alternaria tenuissima Taishan variant metabolite and Taishan mycin can be widely applied to the field of agricultural production and have remarkable economic, social and ecological benefits.

Description

technical field [0001] The invention belongs to the field of biopesticides, and in particular relates to a functional microorganism, in particular to a strain of Alternaria tennatus ( Alternaria alternata ) Taishan variant and its metabolite Taishanmycin, and its application in the prevention and treatment of plant diseases. Background technique [0002] Tomato virus disease is one of the most important diseases that harm tomato production. There are more than 20 kinds of virus pathogens, mainly including Tobacco mosaic virus (TMV) called tobacco mosaic virus, Cucumber mosaic virus (CMV) called cucumber mosaic virus, Tobacco leaf curl virus (TLCV) is called tobacco leaf curl virus, Alfalfa mosaic virus (AMV) is called alfalfa mosaic virus, Tomato yellow leafcurl virus (TYLV) is called tomato yellow leaf curl virus, Potato virus Y (PVY) is called potato virus Y And Whitefly-transmitted geminiviruses (WTG) called tomato whitefly geminiviruses and so on. According to the symp...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/30A01P1/00A01P21/00C05F11/08C05G3/00C05G3/60C12R1/645
CPCC12N1/14A01N63/30C05F11/08C05G3/00C05G3/60Y02A40/10
Inventor 王红艳武玉国姜莉莉王开运于颀王开元
Owner SHANDONG AGRICULTURAL UNIVERSITY
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