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Nitric oxide electrochemical sensing microelectrode and preparation method and application thereof

A nitric oxide and micro-electrode technology, applied in the field of micro-electrodes, can solve the problem that the sensitivity and selectivity cannot meet the high-selectivity detection and so on.

Pending Publication Date: 2021-09-14
NANJING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

By layering multiple types of membranes on top of the electrodes, the selectivity of the sensor can be tuned to distinguish various interferences including nitrite, dopamine, and acetaminophen, enabling the determination of NO concentrations in biological environments, The solid-state catalytic electrode developed on this basis can further reduce the influence of electrical activity interference on the NO selective electrode (Ciszewski A, MilczarekG. Electrochemical detection of nitric oxide using polymer modified electrodes [J]. Talanta, 2003, 61 (1): 11-26.), but its sensitivity and selectivity still cannot meet the high selectivity detection in biological samples

Method used

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  • Nitric oxide electrochemical sensing microelectrode and preparation method and application thereof
  • Nitric oxide electrochemical sensing microelectrode and preparation method and application thereof
  • Nitric oxide electrochemical sensing microelectrode and preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0052] 1. Cut a 15-20cm carbon fiber bundle and soak it in acetone overnight (just make sure the length can pass through the glass tube), and then put it on aluminum foil to dry. Sonicate in pure water for 10-20min, then draw the carbon fiber into a borosilicate glass capillary to prepare a carbon fiber electrode, and draw it to a fine tip with a glass wire drawing machine. The microelectrodes were cut and sealed in epoxy resin (Epon 828), then cured at 80 °C for 2 h and at 150 °C for 2 h, and finally copper wires were attached at the bottom of the carbon fibers to establish electrical contact. Place the treated electrode in 1mM K 3 [Fe(CN) 6 ], the cyclic voltammetry scan was carried out in the voltage range of -0.3 ~ 0.7V, the scan rate was 100mV / s, until the current was stable, and a carbon fiber microelectrode was obtained.

[0053] 2. Sonicate the tip of the carbon fiber microelectrode prepared in step (1) in ultrapure water, absolute ethanol, and ultrapure water for 5 ...

Embodiment 2

[0057] 1. The nitric oxide electrochemical sensing microelectrode prepared in Example 1 was scanned by an electron microscope, and its morphology was characterized under a scanning electron microscope as follows: figure 2 shown by figure 2 It can be seen that there are obvious spherical protrusions on the surface of the microelectrode at the nanoscale, indicating that simple gold was successfully deposited on the surface of the microelectrode, which improved the electrochemical activity of the electrode surface.

[0058] 2. The NO electrochemical sensing microelectrode detects the NO release process of macrophages:

[0059] (1) Nitrogen was introduced into 0.1×PBS solution (pH=7.4) for 20-30min to remove oxygen, and the standard DEANONOate solution (50mM) was diluted and then added in a certain proportion to establish the final concentration gradient of NO (0.1nM, 0.2nM , 0.5nM, 1nM, 2nM, 5nM, 10nM, 20nM, 50nM, 100nM, 200nM, 500nM, 1000nM, 2000nM, 5000nM). Utilize a three-...

Embodiment 3

[0064] Example 3 adopts the method of Example 1, the difference is that in step (3), the microelectrode is incubated in the DNA modification buffer for 12 hours at room temperature, and the heme Hemin and the above-mentioned electrode are combined in the binding buffer (Binding buffer) Incubate at room temperature for 40min. In step (3), the G4-DNA concentration in the DNA modification buffer is 10 μM, and the Hemin concentration in the binding buffer is 0.2 mM.

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Abstract

The invention discloses a nitric oxide electrochemical sensing microelectrode and a preparation method and application thereof. The electrode is characterized in that a microelectrode inner core is formed by carbon fibers, the electrode inner core is sequentially modified by a gold deposition layer, an NO electro-catalysis compound layer and a fluorinated xerogel shielding layer from inside to outside, and the complete microelectrode is formed by the multiple modification layers. According to the prepared NO electrochemical sensing microelectrode, rapid detection of NO is achieved through an electrochemical analysis method, needed electrochemical instruments are simple, selectivity is good, the analysis speed is high, the detection cost is low, microminiaturization is easy to achieve, response is rapid, and monitoring is sensitive. The nitric oxide electrochemical sensing microelectrode provided by the invention is helpful for solving the problems of low selectivity and sensitivity of existing NO detection, is a high-performance NO microsensor, can further expand the NO detection application, and provides important detection and sensing devices for environmental monitoring and NO physiological function research.

Description

technical field [0001] The invention belongs to the field of microelectrodes, and in particular relates to a highly selective nitric oxide (NO) electrochemical sensing microelectrode and a preparation method and application thereof. Background technique [0002] Nitric oxide (Nitric oxide, NO) is an important gas signal molecule in organisms, and plays a key role in processes such as vasodilation, nerve signal transmission and inflammatory response. At the same time, local NO levels are closely related to physiological and pathological states, and play a key indicator role. However, since the active half-life of NO in living organisms is only a few seconds, and the diffusion range is usually within a few hundred microns, the difficulty of NO detection is obvious. Therefore, overcoming the difficulty of NO detection, developing highly sensitive and highly selective NO sensors, and improving the performance of NO sensors can not only promote the research on the physiologica...

Claims

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Application Information

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IPC IPC(8): G01N27/30G01N27/327
CPCG01N27/308G01N27/3275
Inventor 高涛郭嘉荣牟俊慧杨翠徐晨韵
Owner NANJING NORMAL UNIVERSITY
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