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Nano composite material as well as preparation method and application thereof

A nanocomposite material and inorganic nanomaterial technology, applied in the medical field, can solve the problems of decreased ROS generation efficiency, easy recombination of electrons and holes, wide band gap, etc., and achieve the effects of efficient ROS generation, enhanced effect, and high stability.

Pending Publication Date: 2021-12-07
景香香 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But TiO 2 There are also shortcomings that need to be improved, such as wide band gap, easy recombination of electrons and holes, etc., resulting in a decrease in the generation efficiency of ROS

Method used

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  • Nano composite material as well as preparation method and application thereof
  • Nano composite material as well as preparation method and application thereof
  • Nano composite material as well as preparation method and application thereof

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preparation example Construction

[0048] The present invention also provides a method for preparing the nanocomposite material described in the above technical solution, comprising the following steps:

[0049] The two-dimensional structure Ti 3 C 2 Nanosheets, positively charged CuO 2 The nanodots are mixed with bovine serum albumin (BSA), and the solid and liquid are separated to obtain a nanocomposite material.

[0050] In the preparation method provided by the present invention, the two-dimensional structure Ti 3 C 2 Nanosheets are preferably prepared according to the following steps:

[0051] Ti 3 AlC 2 The powder is sequentially subjected to hydrofluoric acid etching treatment and tetrapropylammonium hydroxide intercalation treatment to obtain a two-dimensional structure Ti 3 C 2 nanosheets; where the etching treatment is aimed at removing Ti 3 AlC 2 In the Al layer, the purpose of the intercalation treatment is to transform the material into ultra-thin two-dimensional nanosheets.

[0052] In ...

Embodiment 1

[0076] 1) Ultra-thin two-dimensional structure Ti 3 C 2 Preparation of nanosheets:

[0077] Preparation of Ultrathin Two-Dimensional Structured Ti by Liquid Phase Exfoliation Method Using Hydrofluoric Acid (HF) Etching and Cationic Intercalation Agent (TPAOH) Intercalation 3 C 2 Nanosheets, the specific process is: mix titanium powder (Ti, 325 mesh), aluminum powder (Al, 325 mesh) and graphite powder (graphite, 300 mesh) at a molar ratio of 2:1:1, ball mill for 10 hours, and Cylindrical Ti under pressure 3 AlC 2 Biscuit: Under the protection of argon (Ar), the biscuit is heated to 1500°C in a high-temperature sintering furnace for sintering, and after two hours of heat preservation and sintering, the furnace is cooled to room temperature (25°C) to obtain a MAX phase ceramic block material ( Ti 3 AlC 2 ); pulverize Ti with a mortar and pestle 3 AlC 2 The ceramic block obtained a powder with a particle size of 300-500nm, 10g of the powder was magnetically stirred at 600...

Embodiment 2

[0089] Ti prepared in embodiment 1 3 C 2 / CuO 2 -BSA nanoparticles (Ti 3 C 2 / CuO 2 / BSA molar ratio is 1:2:2) biosafety evaluation:

[0090] 1) Culture U87 glioma cells and HUVEC human vein endothelial cells in vitro, and evaluate Ti 3 C 2 / CuO 2 -BSA nanoparticles are toxic to tumor cells, and the specific process includes:

[0091] Set up two 96-well culture plates, each plate is divided into 7 groups, and each group is set up with 5 multiple wells; add 100uL to each well of plate A at a density of 1×10 4 Glioma cells, add 100uL to each well of plate b with a density of 5×10 3 Human vein endothelial cells, cultured for 24 hours, after the cells adhered to the wall, added equal dilutions of different concentrations of Ti 3 C 2 / CuO 2 -BSA nanoparticles, continue to culture for 24 hours, then add CCK-8 to incubate for 1 hour, detect the cell viability in each well, and measure the absorbance at 450nm with a microplate reader.

[0092] Ti 3 C 2 / CuO 2 -BSA nano...

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Abstract

The invention belongs to the field of medicine, and particularly relates to a nano composite material as well as a preparation method and application thereof. The nano composite material provided by the invention comprises an inorganic nano material and BSA (Bovine Serum Albumin) molecules modified on the surface of the inorganic nano material; and the inorganic nano material comprises a Ti3C2 nanosheet with a two-dimensional structure and CuO2 nanodots which are loaded on the surface of the Ti3C2 nanosheet with the two-dimensional structure and are provided with positive charges. Based on the principle of positive and negative charges, CuO2 is loaded on the surface of the Ti3C2 nanosheet with the two-dimensional structure, and efficient ROS generation is realized by utilizing a Fenton reaction triggered by specific self-supply H2O2 of CuO2 in a tumor microenvironment and exogenous ultrasonic stimulation, so that the tumor treatment effect is synergistically improved. The nano composite material provided by the invention is suitable for sonodynamic and chemical dynamic synergistic and efficient treatment of tumors, especially glioma, and has important significance for the development of clinical tumor treatment and the progress of nano ultrasonic medicine.

Description

technical field [0001] The invention belongs to the field of medicine, and in particular relates to a nanocomposite material and its preparation method and application. Background technique [0002] In 1989, Yumita et al. used ultrasound to stimulate photosensitizers commonly used in photodynamics: hematoporphyrin and its derivatives, and found that they would also produce significant cytotoxic ROS, leading to apoptosis / necrosis of cancer cells. This new tumor treatment method is called sonodynamic therapy (SDT). Reactive oxygen species (ROS) are chemically active molecules, including singlet oxygen ( 1 o 2 ), superoxide radicals (O 2 · - ), hydroxyl radicals (·OH) and peroxides (O 2 - ), which play an important role in the cell life cycle. At lower concentrations, ROS can act as key signaling molecules in cells to regulate cell growth, proliferation, and differentiation. However, abnormal production of ROS in cells will lead to oxidative damage to lipids, proteins o...

Claims

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Application Information

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IPC IPC(8): A61K41/00A61K47/64A61P35/00B82Y5/00B82Y40/00
CPCA61K41/0033A61K41/0057A61K47/643A61P35/00B82Y5/00B82Y40/00A61K2300/00Y02A50/30
Inventor 景香香张敏陈其青杨大艳冯桂英孙丹丹覃伶伶林凌
Owner 景香香
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