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Lactobacillus buchneri and application thereof in preparation of low-moisture fermented feed

A technology of Lactobacillus brucei and fermented feed, applied in the direction of bacteria and Lactobacillus used in food preparation, can solve the problems of loss of nutrients such as vitamins, difficulty in stirring, insufficient fermentation, etc., to improve production performance, Enhance the anti-mildew effect and solve the effect of high moisture content

Active Publication Date: 2021-12-10
BEIJING CHALLENGE AGRI SCI & TECH CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There have been many studies on the fermented feed of a single raw material, and the application of fermented full-price feed is more convenient, but there are two problems in actual production. One is that the direct fermentation of full-price feed as the fermentation substrate may easily lead to the loss of nutrients such as vitamins; The second is that the conventional fermentation process is prone to insufficient fermentation, the degree of fermentation is shallow, and the moisture is more than 30%. High moisture is not only difficult to stir during use, but also prone to mildew during use and storage.
The third is the lack of high-quality fermentation strains or starters. Strains are the key factors in feed fermentation technology, and the quality of strains directly determines the effect and quality of fermented feed.

Method used

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  • Lactobacillus buchneri and application thereof in preparation of low-moisture fermented feed
  • Lactobacillus buchneri and application thereof in preparation of low-moisture fermented feed
  • Lactobacillus buchneri and application thereof in preparation of low-moisture fermented feed

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Screening of Lactobacillus Brucella

[0028] The samples come from the feces of weaned piglets in our company's experimental pig farm. The samples are expanded in the MRS medium. A single colony was taken for secondary streak separation to obtain 7 different lactic acid bacteria, and then the acid production performance and phenyllactic acid content of the 7 lactic acid bacteria were evaluated respectively, and a strain of lactic acid bacteria with better acid production performance was screened and named as No. 1 lactic acid bacteria. figure 1 The colony morphology of No. 1 lactic acid bacteria (later named Lactobacillus Brucella TZ-LB-017) cultured in MRS medium for 24 hours.

[0029] The separation and purification steps are as follows: weigh 20 g of feces samples under aseptic conditions, transfer them to corresponding 180 mL sterile peptone medium, shake and mix well, let stand for 10 min, and take the supernatant for later use. Take 10 mL of the supern...

Embodiment 2

[0033] Example 2 Verification of the antibacterial effect of this strain of Lactobacillus Brucella strain

[0034] The Oxford cup bacteriostatic test method was used to verify Lactobacillus Brucella ( Lactobacillus buchneri ) TZ-LB-017 antibacterial effect, found that this strain of Lactobacillus Brucella has obvious inhibitory effect on Staphylococcus aureus and coliform bacteria, indicating that this strain of Lactobacillus Brucella can inhibit common harmful bacteria in feed Both gram-negative and gram-positive bacteria have a significant effect.

[0035] In the experiment, Escherichia coli and Staphylococcus aureus were used as indicator bacteria, and blank medium (M) and ampicillin (A) were used as controls to verify the antibacterial effect of this strain of Lactobacillus brucei. see results figure 2 . In the figure, A is the antibiotic (ampicillin) (as a positive control), M is the MRS blank medium (as a control), and LAB is the culture medium of this strain of Lact...

Embodiment 3

[0036] Example 3 Anti-mold Performance Tracking of Fermented Feed Prepared by Different Lactobacillus Brucella

[0037] Lactobacillus Brucella TZ-LB-017 isolated by our company (lactic acid bacteria 1 in Example 1), commercially available Lactobacillus Brucella 40788, commercially available Lactobacillus Brucella KLDS1.0364 and commercially available conventional Lactic acid bacteria are fermented strains, and solid fermentation experiments are carried out to analyze the advantages of different Lactobacillus Brucella strains in the production process of fermented materials. Take 400 parts of soybean meal, 200 parts of corn, and 400 parts of bran, mix them, add 500 parts of water, and after mixing evenly, divide them into 5 parts on average, and inoculate 0.5 parts of normal saline (control group) and 0.5 parts of Lactobacillus brucei TZ respectively. - LB-017 culture solution (test group 1), 0.5 parts of conventional lactic acid bacteria culture solution (test group 2), 0.5 pa...

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Abstract

The invention relates to lactobacillus buchneri and application thereof in preparation of low-moisture fermented feed, and provides lactobacillus buchneri TZ-LB-017, the lactobacillus buchneri is used for preparing mixed bacterial liquid, and the low-moisture fermented feed is prepared through adoption of a two-step method, namely, part of raw materials are inoculated with a bacterial enzyme leavening agent at first, other raw materials, stone powder, calcium hydrophosphate, sodium chloride, amino acid, premix and the like are mixed and then subjected to secondary fermentation, the two-step fermentation can promote the stability of the viable count, is more beneficial to production of organic acid, digestion and absorption of beneficial substances such as enzyme and the like, and is beneficial to the stability of vitamins and mineral substances; the low-moisture fermented feed prepared by adopting the preparation method disclosed by the invention can be prevented from mildewing for at least 90 days, and has remarkable advantages in the aspects of acid production, harmful bacterium inhibition and synergistic effect with other probiotics; and the low-moisture fermented feed disclosed by the invention is mixed with a conventional feed for feeding, and the low-moisture fermented feed accounts for 40-60% of the weight of the mixed feed, so that the low-moisture fermented feed has a remarkable promoting effect on improving the production performance of livestock and poultry.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a lactobacillus brauchneri and its application in the preparation of low-moisture fermented feed. Background technique [0002] Fermented feed has been widely used in many foreign countries. At present, more than 60% of large-scale pig farms in the Netherlands are using fermented feed, more than 30% of sows in Denmark use fermented feed, and about 15% of pig farms in France use fermented feed . The research and application of bio-fermented feed in my country has also become a research hotspot in my country's animal husbandry industry. However, while the fermented feed industry is developing rapidly, there are also many problems in actual production and application. Among them, easy mold and deterioration during storage and use are important factors that hinder the wide application of fermented feed. Mildew will not only lead to a decline in the palatability and nutritio...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/18C12N9/50C12N9/42C12N9/24C12N9/16A23K10/12A23K10/14A23K10/30A23K10/37A23K20/20A23K20/142A23K20/174A23K20/28A23K50/30A23L3/3571C12R1/225C12R1/125C12R1/865
CPCC12N1/20C12N1/18C12N9/50C12N9/2437C12N9/2488C12N9/248C12N9/16C12Y301/03008C12Y301/03026A23K10/12A23K10/14A23K10/30A23K10/37A23K20/20A23K20/142A23K20/174A23K20/28A23K20/30A23K50/30A23L3/3571A23V2002/00A23V2400/11A23V2200/10Y02P60/87
Inventor 华跃飞李爽李阳孙敏王海燕张广民蔡辉益
Owner BEIJING CHALLENGE AGRI SCI & TECH CO LTD
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