Lactobacillus buchneri and application thereof in preparation of low-moisture fermented feed
A technology of Lactobacillus brucei and fermented feed, applied in the direction of bacteria and Lactobacillus used in food preparation, can solve the problems of loss of nutrients such as vitamins, difficulty in stirring, insufficient fermentation, etc., to improve production performance, Enhance the anti-mildew effect and solve the effect of high moisture content
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Embodiment 1
[0027] Example 1 Screening of Lactobacillus Brucella
[0028] The samples come from the feces of weaned piglets in our company's experimental pig farm. The samples are expanded in the MRS medium. A single colony was taken for secondary streak separation to obtain 7 different lactic acid bacteria, and then the acid production performance and phenyllactic acid content of the 7 lactic acid bacteria were evaluated respectively, and a strain of lactic acid bacteria with better acid production performance was screened and named as No. 1 lactic acid bacteria. figure 1 The colony morphology of No. 1 lactic acid bacteria (later named Lactobacillus Brucella TZ-LB-017) cultured in MRS medium for 24 hours.
[0029] The separation and purification steps are as follows: weigh 20 g of feces samples under aseptic conditions, transfer them to corresponding 180 mL sterile peptone medium, shake and mix well, let stand for 10 min, and take the supernatant for later use. Take 10 mL of the supern...
Embodiment 2
[0033] Example 2 Verification of the antibacterial effect of this strain of Lactobacillus Brucella strain
[0034] The Oxford cup bacteriostatic test method was used to verify Lactobacillus Brucella ( Lactobacillus buchneri ) TZ-LB-017 antibacterial effect, found that this strain of Lactobacillus Brucella has obvious inhibitory effect on Staphylococcus aureus and coliform bacteria, indicating that this strain of Lactobacillus Brucella can inhibit common harmful bacteria in feed Both gram-negative and gram-positive bacteria have a significant effect.
[0035] In the experiment, Escherichia coli and Staphylococcus aureus were used as indicator bacteria, and blank medium (M) and ampicillin (A) were used as controls to verify the antibacterial effect of this strain of Lactobacillus brucei. see results figure 2 . In the figure, A is the antibiotic (ampicillin) (as a positive control), M is the MRS blank medium (as a control), and LAB is the culture medium of this strain of Lact...
Embodiment 3
[0036] Example 3 Anti-mold Performance Tracking of Fermented Feed Prepared by Different Lactobacillus Brucella
[0037] Lactobacillus Brucella TZ-LB-017 isolated by our company (lactic acid bacteria 1 in Example 1), commercially available Lactobacillus Brucella 40788, commercially available Lactobacillus Brucella KLDS1.0364 and commercially available conventional Lactic acid bacteria are fermented strains, and solid fermentation experiments are carried out to analyze the advantages of different Lactobacillus Brucella strains in the production process of fermented materials. Take 400 parts of soybean meal, 200 parts of corn, and 400 parts of bran, mix them, add 500 parts of water, and after mixing evenly, divide them into 5 parts on average, and inoculate 0.5 parts of normal saline (control group) and 0.5 parts of Lactobacillus brucei TZ respectively. - LB-017 culture solution (test group 1), 0.5 parts of conventional lactic acid bacteria culture solution (test group 2), 0.5 pa...
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