Bifidobacterium capable of relieving colitis and application thereof

A technology for bifidobacteria and colitis, applied in the directions of bifidobacteria, bacteria used in application and food preparation, etc., can solve the problems of prevention and treatment of ulcerative colitis without ulcerative colitis, achieve the protection of intestinal barrier function and reduce disease Activity index, effect of weight loss

Active Publication Date: 2022-01-07
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there are no effective measures for the prevention and treatment of ulcerative colitis. Existing therapeutic drugs such as aminosalicylic acid, glucocorticoids, and immunosuppressants all have certain side effects. Therefore, research on new alternative treatments method is very important

Method used

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  • Bifidobacterium capable of relieving colitis and application thereof
  • Bifidobacterium capable of relieving colitis and application thereof
  • Bifidobacterium capable of relieving colitis and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0081] Example 1: Screening, Identification, Culture, Observation and Preservation of Bifidobacterium pseudocatenulatum N2

[0082] 1. Screening

[0083]Take 1g of feces samples from healthy infants in Harbin, spread them on MRS solid medium (including rapid screening) after serial dilution, and culture them in an anaerobic environment at 37°C for 72 hours, observe and record the colony morphology; , Protruding, white and yellowish colonies were streaked on the MRS solid medium, and purified and cultivated under anaerobic conditions at 37°C. Repeat this operation 3 times to obtain purified single colonies; pick a single colony on Streak on the MRS solid medium and incubate anaerobically at 37°C for 36h.

[0084] 2. Identification

[0085] Extract the genome of the bacterial strain obtained by screening, amplify and sequence the 16S rDNA of the bacterial strain (the nucleotide sequence of the 16S rDNA obtained by amplification is shown in SEQ ID NO.1), and carry out the obtai...

Embodiment 2

[0091] Embodiment 2: the preparation of pseudo small chain Bifidobacterium (Bifidobacterium pseudocatenulatum) N2 bacterial suspension

[0092] (1) Dip the bacterial solution of Bifidobacterium pseudocatenulatum N2 from the glycerol tube, streak it on the MRS solid medium, culture it at 37°C for 48 hours in an anaerobic environment, and obtain a single colony; pick a single colony for inoculation In the MRS liquid culture medium, culture at 37° C. for 48 hours under anaerobic environment for activation culture, repeat this operation 3 times, and obtain the activated bacterial liquid. (2) Inoculate the activated bacterial liquid obtained in step (1) into the MRS liquid medium according to the inoculum size of 2% (v / v), cultivate at 37°C for 24 hours to obtain a fermentation liquid, and collect the bacteria by centrifugation of the fermentation liquid body, resuspend the cell body with physiological saline, and adjust the number of viable cells to 5×10 9 CFU / mL, made into bacte...

Embodiment 3

[0093] Embodiment 3: the tolerance of pseudosmall chain Bifidobacterium (Bifidobacterium pseudocatenulatum) N2 to digestive tract: bacterial strain is adjusted to 1.0 * 10 9 CFU / mL, placed in gastric juice and incubated for 2h, then transferred to intestinal juice and incubated for another 2h. The survival rate of the experimental strains was calculated as follows:

[0094]

[0095] Note: Among them, N1 is the surviving strain after treatment, and N0 is the total strain without treatment.

[0096] Bifidobacterium pseudocatenulatum N2 has a higher survival rate in the digestive tract, and there is no significant difference with BB-12. This result shows that Bifidobacterium pseudocatenulatum N2 can survive in the digestive tract.

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Abstract

The invention discloses a rapid screening method and application of bifidobacterium capable of relieving colitis and belongs to the technical field of biology. The bifidobacterium pseudocatenulatum is preserved in China Center for Type Culture Collection (CCTCC), Wuhan, on July 5, 2021, and the preservation number is CCTCC M2021820. The bifidobacterium pseudocatenulatum can be used for significantly improving weight loss, colon length and disease activity index of mice during DSS-inducing colitis, enhancing intestinal barrier integrity, and inhibiting tissue damage, myeloperoxidase (MPO) activity, PGE2 level and proinflammatory factors. Compared with mice of a model group, the bifidobacterium pseudocatenulatum KLDS N2 has the advantages that the expression of proinflammatory cytokine mRNA of a DSS stimulated mouse is obviously down-regulated, and the expression of mRNA of mucoprotein and tight junction protein is up-regulated.

Description

Technical field: [0001] The invention relates to a bifidobacterium dentate strain capable of alleviating colitis and application thereof, belonging to the field of biotechnology. [0002] technical background [0003] Ulcerative colitis is a chronic idiopathic intestinal inflammatory disease. Early clinical manifestations are blood-type diarrhea. As the disease continues, symptoms such as diarrhea, blood in the stool, weight loss, and vomiting will appear. In addition, it will also cause complications such as toxic colon dilatation, intestinal perforation, polyps, and enteritis. The incidence of ulcerative colitis in my country is on the rise. [0004] Ulcerative colitis mainly manifests as inflammation of the colorectal mucosa and submucosa. Under normal circumstances, the body is jointly protected by a physical barrier composed of intestinal epithelial cells and surface mucus, a chemical barrier composed of immune-mediated antibacterial substances, and a microbial barrier...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/745A61P1/04A61P1/00A23L33/135A23L2/52C12R1/01
CPCC12N1/20A61K35/745A61P1/04A61P1/00A23L33/135A23L2/52A23V2002/00A23V2400/535A23V2200/3204Y02A50/30
Inventor 李柏良赵莉梁胜男董佳欢霍贵成刘飞王松
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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