Polyurethane degrading fungus strain as well as separation method and application thereof
A technology of polyurethane and fungi, applied in the field of environmental microorganisms, can solve the problems of affecting the reproduction and development of marine organisms, interfering with toxicity, etc., and achieve the effects of fast growth and proliferation, low cost, and easy cultivation
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0062] Example 1. Bacterial strain isolation and purification
[0063] Prepare enriched culture medium (using polyurethane as the sole carbon source). 1 L of enriched media formulated to include: 7 g K 2 HPO 4 ; 2g KH 2 PO 4 ; 1g (NH 4 ) 2 SO 4 ;0.1MgSO 4 ·7H 2 O; 0.001g ZnSO 4 ·7H 2 O; 0.0001g CuSO 4 ·5H 2 O; 0.01g FeSO 4 ·7H 2 O; 0.002g MnSO 4 ·7H 2 O; 0.3% polyurethane. Make up to 1L with distilled water. The pH was adjusted to 6.0. After the prepared culture solution was sterilized and cooled, ampicillin and chloramphenicol (used to inhibit the growth of bacteria in the sample) were added at a final concentration of 100 μg / mL, and mixed uniformly to prepare an enriched culture solution.
[0064] Enrichment culture. Dilute the eastern Pacific deep-sea sediment sample by 10 -1 , and then pipette 1 mL of the diluted sample, pour it into the above-mentioned enrichment culture medium, and culture it with shaking at 28°C under the condition of sufficient oxy...
Embodiment 2
[0066] Example 2. Biological identification of fungi
[0067] The biological identification of fungi is mainly to identify the microstructure of the hyphae of its fungi, and the specific steps are as follows: first prepare corn flour agar medium (CMA, see the above table 1), and pour the plate with the prepared medium, Try to be as thin as possible when pouring flat plates. Pick a ring of colonies with an inoculation loop, and make a "zigzag" streak on the medium. Cut out two rectangles of medium in the streaked medium with a scalpel, take it out, put a cover glass on it, and culture it in a 25°C incubator for 7 days. Place the petri dish on an inverted microscope (Olympus IX51), find representative colonies, and take pictures.
[0068] figure 1 The colony characteristics presented by the Cladosporium halosporium strain of the present invention on the solid plate medium are shown: the early colony is round, loose in texture, white velvet, the middle part of the colony is gr...
Embodiment 3
[0069] Example 3. Gene sequence analysis
[0070] For fungi that have been pure, scrape off the hyphae, and use FastDNA TM The SPIN Kit for Soil kit was used to extract DNA from strains. The extracted DNA was amplified by the primers of ITS4 (SEQ ID NO:2:5'-TCCGTAGGTGAACCTGCGG-3') and ITS5 (SEQ ID NO:3:5'-TCCTCCGCTTATTGATAGC-3') the ITS sequence of the genome on a thermal cycler , the length of its sequence is about 600bp. The PCR product of the amplified ITS sequence (SEQ ID NO: 1) entrusted Shanghai Meiji Sequencing Co., Ltd. to analyze the gene sequence of the 26S rDNA-ITS region. After successful sequencing, use BioEdit software to remove redundant sequences from the ITS sequence of the fungus and retain the peak The relatively neat part of the sequence, and then Blast analysis and comparison of the fungal ITS sequence, according to the sequence similarity of 98% to 100% as the standard to determine the fungal species status. Combined with the morphology and microscopi...
PUM
| Property | Measurement | Unit |
|---|---|---|
| particle diameter | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More