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Method for rapid colorimetric analysis of total antioxidant capacity based on enzyme activity of MnCo-C NCs and application of method

A technology of total antioxidant capacity and colorimetric analysis, which is applied in the field of colorimetric sensing, can solve problems such as limiting wide application, and achieve the effects of simple preparation process, shortened detection time, and rapid analysis

Pending Publication Date: 2022-07-05
HUNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is still a challenge to obtain enzyme-like active nanomaterials with high catalytic activity, which greatly limits their wide application.

Method used

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  • Method for rapid colorimetric analysis of total antioxidant capacity based on enzyme activity of MnCo-C NCs and application of method
  • Method for rapid colorimetric analysis of total antioxidant capacity based on enzyme activity of MnCo-C NCs and application of method
  • Method for rapid colorimetric analysis of total antioxidant capacity based on enzyme activity of MnCo-C NCs and application of method

Examples

Experimental program
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Effect test

Embodiment 1

[0040] A method for rapid colorimetric analysis of total antioxidant capacity based on the enzymatic activity of MnCo@C NCs, including the following steps:

[0041] (1) Preparation of MnCo@C NCs composite nanomaterials:

[0042] (1.1) Manganese acetate and PVP (K-30) are dispersed in a mixed solution of ethanol and water and stirred, then, an aqueous solution of potassium cobalt cyanide is added, the mass ratio of manganese acetate and PVP is 1:5, the mole of Mn and Co The ratio is 1:1, and the MnCo precursor is obtained by incubating at room temperature;

[0043] (1.2) redispersing the prepared MnCo precursor into an aqueous solution, then adding an aqueous solution of dopamine, and the mass ratio of dopamine to MnCo precursor is 1:1 to obtain;

[0044] (1.3) The MnCo@C NCs obtained in step (1.2) are obtained by pyrolysis at high temperature.

[0045] like figure 1 The electron microscope imaging analysis of MnCo@C NCs shows that the MnCo@C NCs have a core-shell structure ...

Embodiment 2

[0056] Wherein, in step (1), the mass ratio of manganese acetate to PVP is 1:10, and the molar ratio of Mn to Co is 1:3, and incubated at room temperature to obtain MnCo precursor; the mass ratio of dopamine to MnCo precursor is 1:3.

[0057] The rest are the same as in Example 1.

[0058] It should be noted that the properties of the MnCo@CNCs prepared in Example 2 are the same as those of the MnCo@CNCs prepared in Example 1, and also have good oxidase-like activity.

[0059] It can be seen that the rapid colorimetric analysis method for total antioxidant capacity based on the oxidase-like activity of MnCo@C NCs composite nanomaterials of the present invention can be used to qualitatively and quantitatively detect AA concentration, and can analyze food according to linear regression equation. the TAC.

[0060] actual sample analysis

[0061] To evaluate the reliability of the rapid colorimetric analysis method for total antioxidant capacity based on composite nanomaterials...

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Abstract

The invention discloses a method for rapid colorimetric analysis of total antioxidant capacity based on enzyme activity of MnCo-C NCs and application of the method, and belongs to the technical field of colorimetric sensing. The method comprises the following steps: firstly preparing a MnCo-C NCs enzyme-like active material with a core-shell structure, then adding the MnCo-C NCs enzyme-like active material into a plurality of sodium acetate-acetic acid buffer solution samples containing different concentrations of AA for incubation by utilizing the enzyme-like activity of the MnCo-C NCs enzyme-like active material, adding a chromogenic substrate TMB for reaction, and recording an absorbance value A at the wavelength of 652nm to obtain a detection linear relationship between the concentration of AA and the absorbance difference value delta A; aA is used as an antioxidant mode compound, and mM / L AA is used as a unit to characterize TAC. The colorimetric detection method has the advantages of low cost, simplicity and convenience in operation, quick response, strong anti-interference capability, high sensitivity and the like, and can be applied to determination of food TAC.

Description

technical field [0001] The invention belongs to the technical field of colorimetric sensing, and relates to a colorimetric analysis method for total antioxidant capacity, in particular to a method for rapid colorimetric analysis of total antioxidant capacity based on MnCo@C NCs enzyme activity and its application in food safety detection application. Background technique [0002] The production and increase of intracellular reactive oxygen species (Reactive Oxygen Species; ROS) such as superoxide radical anion, hydrogen peroxide, hydroxyl radical, semiquinone radical, singlet oxygen, etc. will lead to cell senescence or apoptosis. Scientific research shows that cancer, aging or other diseases are mostly related to the production of excess free radicals, and research on antioxidants can effectively overcome the harm caused by them. Total Antioxidant Capacity (TAC) is used to describe the ability of antioxidants in different foods to scavenge harmful free radicals in the bloo...

Claims

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Application Information

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IPC IPC(8): G01N21/78C01G51/00C01B32/00B82Y30/00
CPCG01N21/78C01G51/00C01B32/00B82Y30/00
Inventor 汤琳朱旭陈丽卢雅婷
Owner HUNAN UNIV
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