Ratio-dependent fluorescent probe for detecting residual quantity of 2, 4-D and determination method

A fluorescent probe and measurement method technology, applied in the field of analytical chemistry, can solve the problems of high equipment cost, long analysis time and high sensitivity, and achieve the effects of high biocompatibility, accurate test results and good reproducibility

Pending Publication Date: 2022-07-29
TIANJIN UNIV
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Problems solved by technology

[0004] Aiming at the problems of long analysis time and high equipment cost in existing 2,4-D detection methods, the present invention discloses a method for detecting 2,4-D residues in vegetables with a ratiometric fluorescent probe. The method has high sensitivity and high selectivity, and can realize the visual detection of 2,4-D by using ultraviolet light, which has a good application prospect

Method used

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  • Ratio-dependent fluorescent probe for detecting residual quantity of 2, 4-D and determination method
  • Ratio-dependent fluorescent probe for detecting residual quantity of 2, 4-D and determination method
  • Ratio-dependent fluorescent probe for detecting residual quantity of 2, 4-D and determination method

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preparation example Construction

[0048] The preparation steps of the blue fluorescent carbon dot CDs include: dissolving citric acid and 0.52 g polyvinylamide in ultrapure water to obtain a mixed solution; then transferring the mixed solution to a polytetrafluoroethylene autoclave, After heating the mixed solvent for a certain period of time, it is cooled to room temperature, and the cooled solution is centrifuged to obtain a carbon dot solution; the carbon dot solution is filtered and purified to obtain the blue fluorescent carbon dot CDs. The citric acid and polyvinylamide are respectively 0.5-2 g and 0.4-0.6 g, and the ultrapure water is 20 mL; the heating temperature is 200-220° C., and the heating time is 3-4 hours. The purification is carried out in a dialysis bag (MW 1KDa) for 12-15 hours, and small molecule impurities are removed by purification.

[0049] The technical scheme of the present invention designs a dual emission ratio fluorescent probe with good photostability, biocompatibility, self-elimi...

Embodiment 1

[0051] This embodiment provides a dual emission ratio fluorescent probe, and the required materials include: MnO 2 OPDox with yellow fluorescence and blue CDs produced by nanosheet oxidation of OPD. The MnO 2 The specific method of nanosheets: 10mL MnCl 2 (0.3M) with 20mL H 2 O 2 (3%) and tetramethylammonium hydroxide (TMAH) (0.6M) were rapidly mixed; the mixture was stirred at room temperature for 12h, then the mixture was centrifuged at 2000rpm for 20min, washed three times with ultrapure water and methanol; The manganese dioxide flakes were dried at 60°C for subsequent experiments. The dried large pieces of manganese dioxide (1 mg / mL) were ultrasonically dispersed for 12 h, centrifuged at 2000 rpm for 30 min, and the supernatant was taken and stored at 4°C for later use. The specific procedure for the blue CDs: 1.0 g of citric acid and 0.52 g of polyacrylamide were first dissolved in 20 mL of ultrapure water, and then the mixed solution was transferred to a polytetrafl...

Embodiment 2

[0053] This embodiment provides a method for detecting 2,4-D using a dual emission ratio fluorescent probe, specifically:

[0054] (1) MnO prepared in Example 12 Take 200 μL of nanosheets (0.2 mg / mL), add 150 μL of LPD (10 mM) and 40 μL of carbon quantum dots, and react in pH 5 PBS buffer for 5 min. Obtain the probe solution to be detected.

[0055] (2) 100μL AAP (3mM) and 100μL ALP (20mU / mL) were added to PBS buffer at pH 8, and different concentrations of 2,4-D were added to the reaction system and incubated at 37°C for 30min. The detection probe solution is added to the mixed solution, mixed evenly, the volume is adjusted, and finally transferred to a cuvette for detection. The final concentrations of 2,4-D in the control reaction system were: 0 μg / mL, 0.05 μg / mL, 0.1 μg / mL, 0.25 μg / mL, 0.5 μg / mL, 1 μg / mL, 5 μg / mL, 10 μg / mL, 15 μg / mL, 20 μg / mL, 30 μg / mL, 40 μg / mL, 50 μg / mL.

[0056] (3) Based on the ratio of the fluorescence intensities of the two probe materials in the...

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Abstract

The invention discloses a ratio-type fluorescent probe and a determination method for 2, 4-D residual quantity detection, and the determination method comprises the following steps: in a buffer solution, a yellow fluorescent oxidation product OPDox and a blue fluorescent carbon dot CDs are used as two probes, and the yellow fluorescent oxidation product OPDox is obtained after colorless o-phenylenediamine OPD is oxidized by a MnO2 nanosheet; establishing a linear relationship between the concentration of 2, 4-D and the fluorescence intensity ratio of the two probes; and testing by using a fluorescence spectrometer to obtain the fluorescence intensity of the two probes in the reacted solution, and calculating to obtain the concentration of 2, 4-D in the to-be-detected sample. The yellow fluorescent oxidation product OPDox and the blue fluorescent carbon dots CDs are used as ratio type fluorescent probes. The ratio-dependent fluorescent probe provided by the invention is quick in response to 2, 4-D detection and stable in color, can realize high-sensitivity and high-selectivity detection of 2, 4-D pesticide residues in samples such as vegetables and fruits, and has the advantages of simplicity and convenience in operation, low cost, sensitive response, easiness in quick visual detection and the like.

Description

technical field [0001] The invention relates to the field of analytical chemistry, in particular to a ratio-type fluorescent probe and a determination method for the detection of 2,4-D residues. Background technique [0002] 2,4-Dichlorophenoxyacetic acid is abbreviated as 2,4-D. It is a derivative of phenolic compounds. It belongs to the phenoxyacetic acid class of herbicides. It is also a synthetic auxin. Grass, which can be used for the preservation of fruits and vegetables at a low concentration, is widely used because of its low dosage and low cost. Improper use of 2,4-D will cause its residues in water, soil or fruits and vegetables, which will cause harm to the environment and human health. In addition to the acid form, phenoxyacetic acid herbicides also have three forms: inorganic salts, amine salts and ester salts. These compounds will decompose into 2,4-D under certain conditions. Auxin herbicides have hepatotoxic and nephrotoxic effects in humans and animals, an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64C09K11/65C09K11/06
CPCG01N21/6428G01N21/643C09K11/06C09K11/65C09K2211/1007
Inventor 赵友全房彦军郭阳何峰张秋彭波刘金凤
Owner TIANJIN UNIV
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