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Method for synchronously extracting and separating lycium barbarum protein and polysaccharide

A technology for synchronous extraction of wolfberry protein, applied in peptide preparation methods, chemical instruments and methods, bulk chemical production, etc., can solve problems such as unfavorable simultaneous extraction of wolfberry protein, extraction of polysaccharides and proteins, and destruction of wolfberry protein. Simple, high extraction rate, efficient extraction and separation effect

Pending Publication Date: 2022-08-09
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

For example, Chinese patent document CN113698506A discloses a temperature-responsive deep eutectic solvent and a method for extracting Lycium barbarum polysaccharide. The deep eutectic solvent is formed by mixing hydrogen bond donors and hydrogen bond acceptors. One of n-decanoic acid, lauric acid, and myristic acid, and the hydrogen bond acceptor is one of tetracaine, lidocaine, and procaine. This deep eutectic solvent will destroy wolfberry protein, which is not conducive to wolfberry Simultaneous extraction of protein and polysaccharides has the disadvantage of high cost; Chinese patent document CN112457426A discloses a method for simultaneously extracting different Lycium barbarum polysaccharide components, which is to mix Lycium barbarum with a deep eutectic solvent aqueous solution and degrease to obtain Lycium barbarum slag, and then add water for homogeneous extraction. The deep eutectic solvent is a mixture of menthol and lactic acid. The principle is that small molecules such as flavonoids, glycosides, alkaloids and monosaccharides in wolfberry preferentially bond with hydrogen bonds in the deep eutectic solvent. Receptor binding, most of which are removed to facilitate the extraction of polysaccharides, the deep eutectic solvent cannot use hydrogen bonds to extract polysaccharides and proteins, and menthol and lactic acid also have high costs

Method used

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  • Method for synchronously extracting and separating lycium barbarum protein and polysaccharide
  • Method for synchronously extracting and separating lycium barbarum protein and polysaccharide
  • Method for synchronously extracting and separating lycium barbarum protein and polysaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0083] Example 1 Extraction of Lycium barbarum protein and polysaccharide by deep eutectic solvent at different temperatures

[0084] Prepare defatted wolfberry powder as follows:

[0085] a) Place the wolfberry fruit in a cool, dry and ventilated place to air dry naturally, bake it in an oven at a temperature of 60°C until the water content of the wolfberry fruit is about 10%, then pulverize it with a pulverizer, and pass it through a 100-mesh sieve to obtain wolfberry powder;

[0086] b) Degreasing the wolfberry powder obtained in step a) with n-hexane, mixing the wolfberry powder and n-hexane according to a mass-volume ratio of 1 g: 2 mL, stirring intermittently at 55° C. for 4 h, cooling to room temperature, and centrifuging at 4000 rpm for 10 min. The sediment was dried in a fume hood, pulverized with a pulverizer, and passed through an 80-mesh sieve to obtain defatted wolfberry powder.

[0087] Prepare the deep eutectic solvent as follows:

[0088] Sodium acetate, urea...

Embodiment 2

[0099] Example 2 Extraction of Lycium barbarum protein and polysaccharide by deep eutectic solvent at different extraction times

[0100] The preparation method of defatted wolfberry powder and low eutectic solvent is the same as that in Example 1.

[0101] Simultaneously extract and separate Lycium barbarum protein and polysaccharide according to the following steps:

[0102] (1) Weigh 0.5000g of defatted Lycium barbarum powder into a conical flask, add 5mL of distilled water to dissolve, then add 15mL of deep eutectic solvent to it and mix evenly, and stir magnetically for 60min, 120min, 180min, and 240min in a water bath at 45°C, respectively. , 300min, centrifuged at 6000rpm for 10min to obtain Lycium barbarum extract;

[0103] Steps (2) to (4) are the same as in Example 1.

[0104] The protein extraction rates obtained under different extraction times in this example are shown in Table 2.

[0105] Table 2 Effect data under different extraction times

[0106] ...

Embodiment 3

[0108] Example 3 Extraction of Lycium barbarum protein and polysaccharide by deep eutectic solvent under different water additions

[0109] The preparation method of defatted wolfberry powder and low eutectic solvent is the same as that in Example 1.

[0110] Simultaneously extract and separate Lycium barbarum protein and polysaccharide according to the following steps:

[0111] (1) Weigh 0.5000g of defatted wolfberry powder and place it in a conical flask, add 1.5mL, 3mL, 4.5mL, 6mL, 7.5mL of distilled water to dissolve, then add deep eutectic solvent (distilled water and deep eutectic solvent together) 15mL) mixed uniformly, magnetically stirred for 180min under 45°C water bath conditions, and centrifuged at 6000rpm for 10min to obtain Lycium barbarum extract;

[0112] Steps (2) to (4) are the same as in Example 1.

[0113] The protein extraction rates obtained under different water addition amounts in this example are shown in Table 3.

[0114] Table 3 Effect data under ...

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Abstract

The invention provides a method for synchronously extracting and separating lycium barbarum protein and polysaccharide, and relates to the field of food deep processing. The method comprises the following steps: extracting a wolfberry raw material by using a deep-eutectic solvent to obtain a wolfberry extract, the deep-eutectic solvent being composed of a hydrogen bond donor and a hydrogen bond acceptor, the hydrogen bond donor being urea and water, and the hydrogen bond acceptor being sodium acetate; separating out lycium barbarum protein and polysaccharide in the lycium barbarum leach liquor by using ethanol to obtain a mixture of the lycium barbarum protein and the polysaccharide; separating the mixture of the lycium barbarum protein and the lycium barbarum polysaccharide to respectively obtain the lycium barbarum protein and the lycium According to the method, the protein and polysaccharide can be synchronously extracted and separated from the lycium barbarum, the extraction rate and purity of the obtained lycium barbarum protein and polysaccharide are obviously higher than those of a single extraction method, and the method has the advantages of high efficiency, low energy consumption, low cost, convenience in operation, novelty, greenness, no discharge of three wastes, recyclable solvent and the like.

Description

technical field [0001] The invention relates to the field of food deep processing, in particular to a method for simultaneous extraction and separation of wolfberry protein and polysaccharide. Background technique [0002] Lycium barbarum is the dry ripe fruit of the perennial deciduous shrub of Solanaceae. It is cultivated in North Korea, Japan, and most of Europe, and China is mainly distributed in Ningxia, Gansu, Xinjiang, Qinghai, Shaanxi, Shanxi and Hebei. "Chinese Pharmacopoeia" states that only Ningxia wolfberry is used in traditional Chinese medicine, so China's Ningxia wolfberry is the most famous. [0003] Lycium barbarum also has immune regulation, anti-oxidation, anti-aging, anti-fatigue, anti-tumor, anti-radiation damage, lowering blood lipids, lowering blood sugar, lowering blood pressure, protecting liver, strengthening brain, kidney, detoxification, beauty and reproductive system protection. Function. Modern Chinese medicine research shows that the main ac...

Claims

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Application Information

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IPC IPC(8): C07K1/14C07K1/30C08B37/00
CPCC07K1/145C07K1/30C08B37/0003Y02P20/54
Inventor 崔建东张泽洋王梓源
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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