Short corynebacteria pharmaceutics or short corynebacteria pharmaceutics without cells in application for preparing medicine of curing HIV infection or AIDS
A technology of short coryneform bacteria and cell-free preparations, applied in the field of medicine, can solve the problems of weakened ability to deal with antigens, reduced ability, abnormal B cell function, etc., and achieve improved absorption and diffusion, improved absorption and diffusion, The effect of uniform particle size
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Embodiment 1
[0084] According to the method described in the 2000 edition of "China Biological Products Regulations", the establishment, verification and other verification of bacterial seed batches were carried out.
[0085] The medium used in this example is the thioglycolate medium (without agar) produced by Beijing Sanyao Science and Technology Development Company, and its ingredients are: tryptone 15g / l, yeast extract powder 5g / l, glucose 5g / l , sodium chloride 2.5g / l, L-cystine 0.5g / l, sodium thioglycolate 0.5g / l. The medium was filtered through a 0.22 μm membrane before use.
[0086] Open the CP (Propionibacterium acnes, scientific name, propionibacterium acnes) 771 (see "China Biological Products Regulations") strain tube, each tube contains 6 billion bacteria, and draw about 0.2-0.3ml of thioglycolate medium (not containing agar), add it to the bottom of the strain tube to dissolve it completely, suck it out, add it to the middle tube (capacity 38ml), mix it with 8-10ml of the ab...
Embodiment 2
[0091] Referring to the method of Example 1, CP (scientific name propionibacterium acnes, propionibacterium acnes) 65101 (76-27) was inoculated in a nutrient tank after 4 consecutive passages, and was cultured in a thioglycolate medium (without agar) (before use) Filter the culture medium with a 0.45 μm membrane) and culture at 36-37°C for 6 days; check the bottles one by one under the microscope, and discard those contaminated by bacteria; combine and collect the bacteria without bacteria contamination, heat and boil for 60 minutes, and use ultra-high pressure jet Collider at 1500kgf / cm 2 Broken bacterium under pressure 5 times; The broken suspension was centrifuged at 8000rpm for 1 hour, washed with sterile normal saline for 5 times, sterility test (2000 edition "China Biological Products Regulation") and pyrogen detection (2000 edition Pharmacopoeia of the People's Republic of China ") is qualified to make a suspension with a solid content of 2.0mg / ml, and heat at 65°C for ...
Embodiment 3
[0095] With reference to the method of Example 1, CP (Propionibacterium acnes) 65102 (H-84) was inoculated in a nutrient tank after 4 consecutive passages, and cultured in a peptone medium at 36-37°C for 7 days; bottle by bottle Microscopic examination, those with contamination by miscellaneous bacteria were discarded; combined and collected bacterial cells without contamination by miscellaneous bacteria were heated and boiled for 30 minutes. 2 Broken thalli under pressure 10 times; The suspension after broken is centrifuged at 12000rpm, washes precipitate 4 times with sterile normal saline, sterility test (2000 version "Chinese Biological Products Regulations") and pyrogen detection (2000 version "People's Republic of China Regulations") Pharmacopoeia ") qualified to make a suspension with a solid content of 1.0mg / ml, heated at 65°C for 100 minutes to obtain NCP.
[0096] Detect (solid content 1mg / ml) NCP pyrogen 60EU / ml by limulus reagent method (2000 edition "Pharmacopoeia ...
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