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Production method for xylose by enzyme process

A xylose and enzymatic technology, applied in the field of bioengineering, can solve problems such as high salt content, high acidity of neutralization solution, increase in production costs of decolorization process and ion exchange process, etc., and achieve the effect of simple process and high purity

Active Publication Date: 2006-07-19
JINAN GUOLI BIOLOGICAL SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The neutralization end point of the neutralization process is not easy to grasp. The high alkalinity of the neutralization solution will destroy the sugar and produce brown color. The high acidity of the neutralization solution will corrode the equipment.
[0009] 3. Due to the addition of a large amount of inorganic acid and inorganic lye in the production process of xylose extracted by acid method, a large amount of inorganic ions and pigments are added to the hydrolyzate, the hydrolyzate is deeply colored, and the salt content is high, which increases the decolorization process and Production cost of ion exchange process
Due to the use of biological enzymes to degrade corncobs and hemicellulose-containing plant stalks (such as corn stalks, wheat straw, bagasse, etc.) Xylanase needs to be fermented and produced by screening specific strains, so far, no relevant literature reports have been found

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] Example 1. Seed culture

[0070] According to corn steep liquor 2.5% (v / v), liquid hemicellulose 4.5% (v / v), bran 2.5% (w / v), magnesium sulfate 0.5% (w / v), dipotassium hydrogen phosphate 0.5% ( w / v), pH is 4.6 formula, prepare seed culture medium, after sterilizing at 120°C for 40min, inoculate Aspergillus niger strain, culture in shake flask at 30°C for 24h, take the liquid after centrifugation, which is the bacteria producing xylanase kind.

Embodiment 2

[0071] Embodiment 2. fermentation prepares xylanase

[0072] According to corn steep liquor 2.5% (v / v), liquid hemicellulose 4.5% (v / v), bran 2.5% (w / v), magnesium sulfate 0.5% (w / v), dipotassium hydrogen phosphate 0.5% ( w / v), PH is the formula of 4.6, prepare fermentation medium 20T in 30T fermenter, after 120 ℃ of sterilization 40min, inoculate xylanase bacterial classification, inoculum size is 5% (v / v), in Ferment at 30°C for 48 hours, and the supernatant was concentrated 10 times by ultrafiltration to obtain 2T concentrated xylanase. The enzyme activity is 20000UI / ml.

Embodiment 3

[0073] Embodiment 3. Production of xylose

[0074] (1) Get 1kg of corn cob and grind it to a particle diameter of 2-5mm, and use 0.1% H 2 SO 4 Soak at a temperature of 60° C. for 1 hour, filter out the soaking liquid, and then rinse with tap water to pH 7.0, and the treated corncobs are set aside.

[0075] (2) Add 8 times the weight of water to the treated corncobs, put them into a high-pressure container, cook at 120°C for 3 hours, and discharge.

[0076] (3) Press the high-temperature cooking extract together with the slag into a stainless steel enzymolysis tank, adjust the pH to 4.0, cool to 40°C, keep the temperature constant, stir, and add xylanase for enzymolysis for 5 hours. Xylanase should be added in 3 batches, and the amount added is 100UI / g corn cob. After the enzymatic hydrolysis, the temperature was raised to 80°C for 15 minutes to inactivate the enzyme.

[0077] (4) Decolorization and concentration:

[0078] After filtering the enzymatically hydrolyzed sugar...

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Abstract

The technical scheme for xylose preparation with enzyme comprises: using CGMCC1476 as a Aspergillus niger strain to ferment and prepare excision xylanase that has high activity and needs mild reaction condition; enzymolyzing specially corn core and zylan in straw materail; refining to prepare single-xylose with purity more than 90%. This invention has no pollution to environment and conversion more than 80%.

Description

Technical field: [0001] The invention belongs to the field of bioengineering, and specifically relates to a method for producing monoxylose by using biological enzymes to degrade xylan in corn cobs and plant straws containing hemicellulose (such as corn straw, wheat straw, bagasse, etc.). technical background: [0002] Xylose is a white to yellow crystal or powder extracted from crop wastes such as corn cobs, corn stalks, wheat straw, cottonseed hulls, bagasse, and wood chips. Xylose is a non-calorie sweetener with refreshing sweetness. Xylose is widely used in food, medicine, chemical industry and other industries. It is an ideal sweetener for obese people and diabetics; an additive for low-calorie food; a culture agent for bioengineering; it is also used for dyeing and tanning. [0003] Eating xylose can improve the microbial environment of the human body and improve the body's immunity. The compatibility of xylose and food is very good, adding a small amount of xylose t...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N9/24C12P19/02C12R1/685
Inventor 袁建国高先岭
Owner JINAN GUOLI BIOLOGICAL SCI & TECH
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