The invention discloses a bladder volume measuring method and instrument. The bladder volume measuring method comprises the steps that S1, ultrasonic signals are transmitted in a plurality of directions of the bladder; S2, two reflection signals, reflected by bladder walls on two sides of the corresponding direction, of the ultrasonic signals in each direction are received; S3, the depth of the bladder in the direction is determined according to the time difference of the two reflection signals in each direction; S4, the bladder volume is determined according to the bladder depths in multipledirections. The propagation time of ultrasonic waves in the bladder is measured, and ultrasonic imaging is not needed, so that a measuring device is simplified, and the operation efficiency of the device is improved. In clinical application, results are quicker to obtain, and the efficiency of measuring the bladder volume is improved.