The invention relates to an agricultural plant protection unmanned aerial vehicle capable of guaranteeing the pesticide spraying effect. The plant protection unmanned aerial vehicle comprises a main body, fan blades, a supporting frame and a water storage chamber, the main body further comprises a mixing mechanism, a cover opening mechanism and a stabilizing mechanism, the mixing mechanism comprises a retracting assembly and a stirring assembly, the cover opening mechanism comprises an air cylinder and a sealing plate, and the stabilizing mechanism comprises a third driving motor, a cylindrical driving gear, a rack, a stabilizing plate and a fixing assembly. According to the agricultural plant protection unmanned aerial vehicle capable of guaranteeing the pesticide spraying effect, the water storage chamber is opened through the cover opening mechanism, the water storage chamber is opened through the cover opening mechanism, then a pesticide in the water storage chamber is efficientlystirred through the mixing mechanism, so that in the spraying process of the pesticide, precipitation is not caused, the concentration of the pesticide is kept unchanged, so that the spraying effect of the pesticide is ensured, the growth of crops cannot be influenced, meanwhile, the water storage chamber is fixed to the supporting frame through the stabilizing mechanism and is fixed more firmly,people or crops are prevented from being crashed and injured, when the water storage chamber needs to be taken down, and parts do not need to be detached and can be directly taken down from the supporting frame, so that time and labor are saved.