Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Vitellus immune globulin for preventing prawn virus, tis preparing method and use thereof

A technology of egg yolk immunoglobulin and viral diseases, applied in the direction of antiviral immunoglobulin, immunoglobulin from eggs, antiviral agents, etc., can solve the problem of inability to effectively inhibit pathogenic microorganisms, reduce the actual application effect, and fail to achieve treatment Effectiveness and other issues, to achieve the effect of improving disease resistance, safe use, and direct effect

Inactive Publication Date: 2008-12-17
DALIAN UNIV OF TECH
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] 1. The pathogen of prawn white spot syndrome is a type of virus. There are differences in the white spot disease virus infected by different hosts in different regions. Only one kind of white spot syndrome virus is selected to prepare the antigen. It cannot be applied to infections caused by other virus strains, the actual application range is narrowed, and the effect of inhibiting and killing viruses is weakened
[0010] 2. The nucleic acid immunogen is made without using the white spot syndrome virus gene or its antigenic determinant gene, which brings disadvantages such as uneven antibody titer produced by immunized poultry, unstable yield, and reduced practical application effect
[0011] 3. Only IgY is used as the only active ingredient for the prevention and treatment of white spot syndrome in prawns. Not only the dosage is increased, but the immunity of the infected prawns is reduced, which leads to the entry of other pathogenic microorganisms, but the IgY cannot effectively inhibit the water body or These pathogenic microorganisms existing inside the shrimp body cannot achieve the best therapeutic effect

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Vitellus immune globulin for preventing prawn virus, tis preparing method and use thereof
  • Vitellus immune globulin for preventing prawn virus, tis preparing method and use thereof
  • Vitellus immune globulin for preventing prawn virus, tis preparing method and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Preparation of Crawfish Primary Cells

[0037] Take healthy crayfish without WSBV infection, wash the body surface, soak in 0.01% potassium permanganate solution for 5 minutes, rinse with sterile water, and disinfect the junction of the carapace and abdomen with alcohol cotton balls. Draw 2-3mL anticoagulant (0.14mol / L NaCl, 0.1mol / L glucose, 30mmol / L citric acid, 26mmol / L sodium citrate, 10mmol / LEDTA, pH4.6) with a sterile syringe, collect blood from the sinus, Mix the anticoagulant with the hemolymph and centrifuge, centrifuge at 1500r / min for 10min, discard the supernatant, wash the precipitate twice with L-15 medium, wash with whole culture medium (15% calf serum, 100U / ml penicillin and 100μg / ml streptomycin L-15 culture medium), gently resuspend the lymphocytes uniformly, inoculate into culture flasks or culture plates, culture overnight at 27°C, and set aside.

Embodiment 2

[0039] Target gene cloning

[0040] The VP28 gene was amplified by PCR method, and the sequencing results showed that the gene contained 615 nucleotides, and the homology of sequence fragments of WSSV from different sources in GeneBamk was 100%.

Embodiment 3

[0042] protein induced expression

[0043] The results of SDS-PAGE electrophoresis showed that the strain carrying the recombinant plasmid pGEX-2T produced a specific protein band at the molecular weight of 24KDa, 26KDa, and 28KDa respectively, and the expression amount accounted for more than 40% of the total bacterial protein. , while no corresponding protein band was produced in the control strain.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

This invention refers to cultivating aquatic animal cultivating and, especially, to vitelline immune globulin' s preparation, which is capable to prevent prawn virus disease. The feature is that: preparing a kind mono-antigen and composite antigen with one or several kinds of inactivate strain / attenuated strain / gene subunit immunogen / nucleic acid immunogen, which belong to prawn leucoderma syndrome virus. The egg avian are uaed as biological reactors, immunity obtaining resisting prawn leucoderma syndrome a specificity LgY, which is alone or together with peptidoglycan, probiotics or Chinese herbs medicine, are capable of being active component of feedstuff additives, medicinal dipping agent, or prawn pool dousing agent to prevent from prawn diseases. The advantages are IgY having effect through passive immunity mechanism to inhibit or kill the virus directly and increase prawn 's immunological competence. It has obvious effect in treating leucoderma syndrome for prawn, and safety, environment protecting, convenient etc.

Description

technical field [0001] The invention belongs to the field of biotechnology, relates to the technical field of aquatic animal breeding, and in particular relates to a yolk immunoglobulin (IgY) for preventing and treating prawn virus disease and a preparation method thereof. The IgY can be used as a feed additive or a medicine bathing agent or the active ingredient of the splashing agent to control the white spot syndrome of prawns. Background technique [0002] my country's prawn farming started in the late 1970s. The continuous improvement and perfection of aquaculture production technology made it quickly become one of the pillar industries of the mariculture industry. The density of stocking, coupled with the continuous deterioration of the ecological environment for farming, has made shrimp diseases, especially viral diseases of shrimps, an important factor hindering the development of this industry. From 1993 to 1994, prawn virus disease raged across my country's north a...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07K16/02C07K16/08A61K39/395A61P31/12
Inventor 徐永平
Owner DALIAN UNIV OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com