Application of cultured saussurea medusa and extract thereof in preparation of medicine for treating II type diabetes and its complications

A technology for diabetes and complications, applied in the fields of bioengineering and traditional Chinese medicine modernization, can solve problems such as suspicion, scientific ignorance of traditional Chinese medicine, lack of curative effect of traditional Chinese medicine preparations, etc., to achieve improved sensitivity, short cultivation period, and good product quality controllability Effect

Inactive Publication Date: 2009-01-28
INST OF BIOENG ACAD OF MILITARY MEDICAL SCI OF THE CHINESE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, traditional Chinese medicine preparations lack hypoglycemic drugs with positive curative effects that clinicians and patients are willing to accept, and rarely establish hypoglycemic models recognized by the pharmaceutical community and select internationally recognized positive control drugs with definite curative effects in animal experiments and clinical trials. That is, the scientific nature of traditional Chinese medicine is often ignored or doubted

Method used

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  • Application of cultured saussurea medusa and extract thereof in preparation of medicine for treating II type diabetes and its complications
  • Application of cultured saussurea medusa and extract thereof in preparation of medicine for treating II type diabetes and its complications
  • Application of cultured saussurea medusa and extract thereof in preparation of medicine for treating II type diabetes and its complications

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] The preparation of the boiling water extract (AESM) of embodiment 1 Saussurea jellyfish cell culture

[0041] Take 50g of the culture of fresh Saussurea jellyfish cells TUIP-8 (Chinese invention patent ZL02156868.5, preserved in our laboratory) cultured in shake flasks, grind and extrude in a mortar, and collect the ground juice separately. Take 500mL of deionized water and boil, pour the crushed Saussurea jellyfish cell culture into the boiling water, stir for 2 minutes, pour it on 4 layers of 300-mesh stainless steel wire mesh immediately, squeeze to collect the juice, and mix it with the ground juice, that is The boiling water extract (AESM) of Saussurea jellyfish cell culture was prepared.

[0042] Aliquot AESM into 10mL glass test tubes and freeze at -20°C. Thaw before use and use up once.

Embodiment 2A

[0043] Embodiment 2AESM is used for treating type II diabetes model animal KK / Ay mice

[0044] KK / Ay mice, a model animal of type II diabetes, were purchased from the Experimental Animal Center of the Chinese Academy of Medical Sciences. They were raised separately under the condition of 25° C. under continuous light for 12 hours, and fed high-fat and high-calorie food arbitrarily. The KK / Ay mice with high blood glucose concentration were screened out by measuring the blood glucose of the mice, and were randomly divided into 3 groups, 10 mice in each group, half male and half male. (1) The model group (negative control group) was orally (fed) with normal saline every day, at a dose of 2.5ml / 100g body weight / mouse. (2) Saussurea jellyfish (AESM) treatment group took orally (fed) 1 ml of boiling water extract of Saussurea jellyfish cell culture once a day. (3) The glimepiride treatment group (positive control group) was fed once a day with a dose of 0.5 mg / kg / bird. Tail vein b...

Embodiment 3A

[0045] Embodiment 3AESM can control the blood sugar and insulin level of type II diabetes model KK / Ay mice

[0046] Continuously administered for 28 days, the blood glucose concentration of KK / Ay mice was monitored every week. Experiments showed that oral AESM could well control the blood glucose level of KK / Ay mice ( Figure 1-A and table 1), the blood glucose of the mice in the model group has been maintained at a hyperglycemic state of 20-28mmol / L, and after treatment with glimepiride, the blood glucose concentration of the mice in this group has been maintained at a lower level of about 12mmol / L, while The blood glucose of the mice in the jellyfish Saussurea jellyfish group has been maintained at a level of 8-10mmol / L, which is very close to the normal level (7-8mmol / L), and has a significant difference from the model group (P<0.001). The level of blood sugar was also better than that of the glimepiride group.

[0047] Table 1 The effect of Saussurea sauraceae cell culture...

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Abstract

The invention discloses jellyfish saussurea involucrate cell and extract application in the II-typed diabetes and complication prevention drug, which is characterized by the following: (1) possessing little side-effect, reducing the density of blood sugar and cholesterol, triglyceride and LDL effectively, (2) improving sensitivity of insulin acceptor, (3) protecting valued saussurea involucrate due to culturing plant cell.

Description

technical field [0001] The invention relates to the use of cultured jellyfish Saussurea jellyfish cells and its extracts in the preparation of medicines for treating type II diabetes and preventing its complications, and belongs to the fields of bioengineering and traditional Chinese medicine modernization. Background technique [0002] Diabetes mellitus is a group of endocrine and metabolic diseases whose etiology and pathogenesis have not been fully elucidated, and hyperglycemia is its common hallmark. Disorders of sugar, protein, fat and secondary water and electrolyte metabolism caused by absolute or relative insufficient secretion of insulin. It can involve various systems of the whole body, and even induce many fatal complications, such as infection complications, retinopathy, hypertension, kidney disease, coronary heart disease, lower extremity vascular disease and cerebrovascular disease, etc., seriously affecting people's working ability and threatening human life ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/28A61P3/10
Inventor 胡显文段海峰高丽华陈惠鹏陈金龙郗永又
Owner INST OF BIOENG ACAD OF MILITARY MEDICAL SCI OF THE CHINESE
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