Calcium phosphate composite bone cement containing eucommia leaf extractive and preparing method thereof

An extract and Eucommia leaf technology, applied in the intersection of material science and biomedicine, can solve the problems of reducing the compressive strength of CPC, slowing down the degradation speed of CPC, prolonging the solidification time of CPC, etc., to promote bone formation and clear the extraction method. Effect

Inactive Publication Date: 2007-08-15
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Before the traditional CPC is completely solidified, adding organic substances such as sodium alginate, hydroxypropyl methylcellulose and carboxymethyl cellulose can prevent the CPC from disintegrating, but it prolongs the setting time of CPC and reduces the resistance of CPC. Compressive stren

Method used

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  • Calcium phosphate composite bone cement containing eucommia leaf extractive and preparing method thereof
  • Calcium phosphate composite bone cement containing eucommia leaf extractive and preparing method thereof
  • Calcium phosphate composite bone cement containing eucommia leaf extractive and preparing method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0029] Example 1 Preparation method of calcium phosphate composite bone cement loaded with Eucommia leaf extract

[0030] (1) Preparation of the solid phase part: crush the fresh bovine reticulated bone into 1cm 3 size, rinsed repeatedly with double distilled water, immersed in 30% H at 38°C 2 O 2After being in the solution for 72 hours (changing the solution every 24 hours), wash with double distilled water for 30 minutes, soak in absolute ethanol for 24 hours, wash again with double distilled water for 30 minutes, and soak in acetone for 24 hours to make partially deproteinized and defatted bone grains. Finally, wash with double distilled water for 30 minutes and then dry at 70°C for 3 days. Then calcined at 800°C in a muffle furnace (heating at a speed of 10°C / min) and kept for 6 hours. The calcined bone particles were immersed in 0.3M diammonium hydrogen phosphate (AP) solution, soaked at 70°C for 24 hours, absorbed excess AP solution on the surface with filter paper, a...

Embodiment 2

[0035] Determination of curing time of calcium phosphate composite bone cement loaded with Eucommia leaf extract

[0036] Add a certain amount of eucommia leaf extract to the solidified liquid (ie, the liquid phase) in advance, so that the final concentrations of the eucommia leaf extract in the solidified liquid are 0, 0.152, 0.304, and 0.608 mg / ml respectively. Weigh 1g of the solid phase part, then add the liquid phase part according to the liquid / solid ratio of 0.35ml / g, fully mix evenly with a stainless steel mixing knife on a clean and dry glass plate, and control the ambient temperature at about 23±1°C. The blending time is 60-90 seconds. When the blend is in the form of a paste, fill it into a template with a diameter of 10mm and a height of 2mm, and fill the blend to the level. From the first minute after the end of the reconciliation, put the template into an incubator at 37°C and a relative humidity greater than 90%, and place a needle poke meter with an indenter di...

Embodiment 3

[0040] Determination of Compressive Strength of Calcium Phosphate Composite Bone Cement Containing Eucommia Leaf Extract

[0041] A certain amount of Eucommia leaf extract was added to the solidified solution in advance, so that the final concentrations of the Eucommia leaf extract in the solidified solution were 0 and 0.608 mg / ml respectively. According to the liquid / solid ratio of 0.35ml / g, add the solidification liquid to the solid phase part, fill the mixture into stainless steel molds with an inner diameter of 4mm and a height of 6mm within 60 seconds, compact it firmly, and screw on the compression screw. Put it in a constant temperature box with a relative humidity greater than 30% at 37°C, remove the mold after 1 hour, and immediately immerse the sample in simulated body fluid (SBF) at 37°C, keep the constant temperature for 23 hours, take it out, smooth both ends of the sample, and accurately measure each The diameter and height of a test piece. The compressive stren...

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Abstract

The invention provides a composite calcium phosphate cement containing hydroxyapatite ceramic, beta- tricalcium phosphate, and Eucommia ulmoides leaf extract with concentration of 0.152-0.608mg/ml. The cement is prepared by preparing a solid phase diammonium hydrogen phosphate solution through calcinations twice, preparing a liquid phase containing Na2HPO4 and glass ionomer cement, and adding Eucommia ulmoides leaf extract into the liquid phase at a ratio of 0.35ml/g. the invention with rational design meets the requirement of clinic and osteoblastic growth, and enhance composite calcium phosphate cement effect. The composite cement has good properties such as mechanical strength, good plasticity, biocompatibility and degradability, and has effects in promoting osteoblast proliferation and bone induction. It is used for bone filling, bone defect repair and bone replacement.

Description

technical field [0001] The invention belongs to the intersecting field of material science and biomedicine, and specifically relates to a preparation method of calcium phosphate composite bone cement loaded with Eucommia leaf extract, and the calcium phosphate composite bone cement can be used as a material for filling, repairing and replacing human bone tissue. Background technique [0002] Calcium Phosphate Cement (CPC) is a new type of non-ceramic hydroxyapatite artificial bone material with biological activity. CPC is a new type of self-curing artificial compound formed by mixing the solid phase (one or several phosphate powders plus HA crystals) and the liquid phase (dilute acid or saline) after hydration and crystallization. Bone substitute materials were originally developed as dental materials. Because it overcomes the shortcomings of traditional ceramic hydroxyapatite that cannot be molded arbitrarily and is difficult to degrade, it has quickly become a common mate...

Claims

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Application Information

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IPC IPC(8): A61L27/54A61L27/12
Inventor 徐靖宏谈伟强林军陈虹李万红
Owner ZHEJIANG UNIV
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