Method for producing L-lactic acid and isoduicitol lactobacillus special for the same

A technology of Lactobacillus rhamnosus and lactic acid, applied in the field of L-lactic acid production, can solve the problems of high research difficulty and high production cost, and achieve the effects of improving utilization efficiency, controlling production cost and simplifying raw material processing process.

Active Publication Date: 2008-05-07
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

And because the used raw material of synthetic method is acetaldehyde and highly toxic substance hydrocyanic acid, so synthetic lactic acid is applied to food and can not be widely accepted, and its producti

Method used

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  • Method for producing L-lactic acid and isoduicitol lactobacillus special for the same

Examples

Experimental program
Comparison scheme
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Example Embodiment

[0046] Example 1. Isolation, mutagenesis screening and identification of Lactobacillus rhamnosus CASL CGMCC No. 2183

[0047] 1. Isolation and mutagenesis screening of strains

[0048] 1) Isolation of strains

[0049] A soil sample was obtained from a nearby dairy factory in Jinan, Shandong Province, and 5 grams of the soil sample was weighed into a 250ml conical flask, added with 100ml of physiological saline, thoroughly mixed, diluted 10,000 times with sterile water, and spread on MRS solid medium at 40°C. After culturing, after a single colony has grown, some single colonies will form a transparent circle due to acid production to dissolve the calcium carbonate in the medium around the colony. Pick a single colony with a larger transparent circle, inoculate it in 10ml of fermentation medium, stand at 40°C for 72 hours, measure the L-lactic acid concentration in the fermentation broth, pick a strain with the highest L-lactic acid yield, and inoculate it in MRS Refrigerate ...

Example Embodiment

[0067] Example 2. Production of L-lactic acid by fermentation in a conical flask using Lactobacillus rhamnosus CASL CGMCC No. 2183

[0068] The composition of the culture medium used in this example is as follows:

[0069] Slant medium: glucose 20 g / L, yeast extract 5 g / L, peptone 10 g / L, beef extract 10 g / L, dipotassium hydrogen phosphate 2 g / L, diammonium citrate 2 g / L, acetic acid Sodium 5 g / L, Tween 80 1 mL / L, magnesium sulfate heptahydrate 0.58 g / L, manganese sulfate tetrahydrate 0.25 g / L, calcium carbonate 15 g / L, agar powder 15 g / L. The initial pH of this medium was 6.5. Sterilized at 115°C for 20 minutes.

[0070] Seed medium: glucose 20 g / L, yeast powder 5 g / L, peptone 5 g / L, calcium carbonate 10 g / L. The initial pH of this medium was 6.5. Sterilization was carried out at 115°C for 20 minutes.

[0071] Fermentation medium: 150 g / L of industrial glucose (Zibo Hongyu Industrial Sugar Co., Ltd.), 100 g / L of soybean meal hydrolyzate (the preparation method is the same ...

Example Embodiment

[0077] Example 3. Production of L-lactic acid by fermentation in a conical flask using Lactobacillus rhamnosus CASL CGMCC No. 2183

[0078] The composition of the culture medium used in this example is as follows:

[0079] The slant medium and seed medium were the same as those in Example 2.

[0080] Fermentation medium: 150 g / L of industrial glucose (Zibo Hongyu Industrial Sugar Co., Ltd.), 300 g / L of soybean meal hydrolyzate (the preparation method is the same as that of Example 1), and 75 g / L of calcium carbonate. The initial pH of this medium was 7. Sterilization was carried out at 115°C for 20 minutes.

[0081] The method for producing L-lactic acid by fermentation method of the present invention comprises the following steps:

[0082] (1) slant cultivation: with embodiment 2;

[0083] (2) seed culture: with embodiment 2;

[0084] (3) Fermentation culture: insert 15ml of the seed nutrient solution of step (2) into a 300ml conical flask containing 85ml of fermentation ...

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Abstract

The invention discloses a method producing L-lactic acid and a special lactobacillus rhamnosus which is Lactobacillus rhamnosus CASL CGMCC No.2183. The L-lactic acid can be obtained by cultivating the Lactobacillus rhamnosus CASL CGMCC No.2183. The fermentation culture medium of the strains is provided with a carbon source, a nitrogen source and the neutralizer used for controlling the fermentation liquid pH; the carbon source is glucose of 150 to 200g/L (the initial fermentation concentration); the nitrogen source is soybean meal hydrolysate, soybean meal hydrolysate and corn steep liquor, or soybean cake powder, and can comprise protease by 0.05 to 0.1g/L when the nitrogen source is soybean cake powder; the neutralizer is the calcium carbonate of 75 to 100g/L, the rest is water and the pH of the fermentation culture medium is 5.5 to 7. With glucose as substrate and a transformation rate of 94.5% to 96.5%, the Lactobacillus rhamnosus CASL CGMCC No.2183 produces the L-lactic acid with an optical purity of 97.6% to 98.7% under the condition of 35 DEG C-45 DEG C, with a highest concentration of 235g/L.

Description

technical field [0001] The present invention relates to a method for producing L-lactic acid and its special bacterial strain, in particular to a method for producing L-lactic acid and its special Lactobacillus rhamnosus. Background technique [0002] Lactic acid, also known as dihydroxypropionic acid, is one of the three largest organic acids in the world. As a traditional multi-purpose fine chemical, lactic acid can be used as sour agent, fragrance, preservative, plant growth regulator, biodegradable material, drug and pesticide, etc., used in food, pharmaceutical, brewing, tanning, Textile, environmental protection and agriculture. Lactic acid is a chiral molecule and can be divided into L-lactic acid, D-lactic acid and DL-lactic acid. Polylactic acid made of L-lactic acid as the main monomer has good biocompatibility and biodegradability, and can be made into medical sustained-release materials, biodegradable fibers and biodegradable plastics. L-lactic acid is becomin...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P7/56C12R1/225
Inventor 许平赵博秦加阳马翠卿周帅
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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