Soil neutral sugar Acetylated Aldononitriles derivation capillary gas chromatography analytical method
A technology for gas chromatography analysis and nitrile acetyl ester, which is applied in the field of capillary gas chromatography analysis of sugar nitrile acetyl ester derived from neutral sugars in soil, can solve problems such as unclearness, and achieve accurate and reliable data, good reproducibility, and accurate data. Effect
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[0026] Example 1:
[0027] Determine the content of neutral sugar in the soil as follows.
[0028] 1.1 Instruments and reagents
[0029] Agilent HP 6890 gas chromatograph, equipped with flame ionization detector (FID), DM-1 quartz capillary gas chromatography column (30m×0.25mm×0.25μm), rotary evaporator, freeze dryer, precision heater, centrifuge , Oven, pH meter, etc.
[0030] Eight kinds of neutral sugar standards, such as ribose, rhamnose, arabinose, xylose, glucose, galactose, mannose, fucose, and internal standards of pentaerythritol and inositol are purchased from Sigma-Aldrich and Fluka ; Hydroxylamine hydrochloride was purchased from Sigma-Aldrich, 4-dimethylaminopyridine was purchased from Fluka; Methylene chloride, ethyl acetate, n-hexane and anhydrous methanol were purchased from Dima; Pyridine, trifluoroacetic acid (TFA), hydrochloric acid And acetic anhydride are both analytical grade.
[0031] 1.2 Preparation of solution
[0032] Preparation of standard solution and...
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