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Soil neutral sugar Acetylated Aldononitriles derivation capillary gas chromatography analytical method

A technology for gas chromatography analysis and nitrile acetyl ester, which is applied in the field of capillary gas chromatography analysis of sugar nitrile acetyl ester derived from neutral sugars in soil, can solve problems such as unclearness, and achieve accurate and reliable data, good reproducibility, and accurate data. Effect

Inactive Publication Date: 2008-07-02
SHENYANG INST OF APPLIED ECOLOGY - CHINESE ACAD OF SCI
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Problems solved by technology

However, it remains unclear which hydrolysis and purification procedure is appropriate when derivatizing with glyconitrile acetyl esters

Method used

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  • Soil neutral sugar Acetylated Aldononitriles derivation capillary gas chromatography analytical method
  • Soil neutral sugar Acetylated Aldononitriles derivation capillary gas chromatography analytical method
  • Soil neutral sugar Acetylated Aldononitriles derivation capillary gas chromatography analytical method

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Experimental program
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Embodiment 1

[0027] The content of neutral sugar in soil was determined as follows.

[0028] 1.1 Instruments and reagents

[0029] Agilent HP 6890 gas chromatograph, equipped with flame ionization detector (FID), DM-1 type quartz capillary gas chromatography column (30m×0.25mm×0.25μm), rotary evaporator, freeze dryer, precision heater, centrifuge , oven, pH meter, etc.

[0030] Eight neutral sugar standards, such as ribose, rhamnose, arabinose, xylose, glucose, galactose, mannose, fucose, and internal standards pentylpentitol and inositol were purchased from Sigma-Aldrich and Fluka Hydroxylamine hydrochloride is purchased from Sigma-Aldrich Company, and 4-dimethylaminopyridine is purchased from Fluka Company; Dichloromethane, ethyl acetate, n-hexane and anhydrous methanol are purchased from Dima Company; Pyridine, trifluoroacetic acid (TFA), hydrochloric acid and acetic anhydride were analytically pure.

[0031] 1.2 Preparation of solution

[0032] Preparation of standard solution and ...

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Abstract

The invention relates to a method for detecting carbohydrates in soil, specifically to a capillary gas chromatographic analysis method for neutral sugars in soil based on acetylated aldononitrile derivatives. The method comprises (1) hydrolysis of a soil sample by weighing a soil sample containing 4-5mg organic carbon, hydrolyzing with 9-12mL 4M trifluoroacetic acid, adding 100Mug pentitol solution, shaking, and filtering to obtain a hydrolysate; (2) purification of the hydrolysate by neutralizing the hydrolysate with potassium hydroxide, adjusting pH to 6.6-6.8, centrifuging, drying by rotary evaporation, dissolving the obtained solid matter in 3-4mL distilled water, adding 100Mug inositol solution, and freeze drying to obtain a dried sample; and (3) derivation of the sample by acetylated aldononitrile method, and detecting by gas chromatography. The invention has the advantages of high sensitivity, good precision and accuracy, and rapid operation; and is suitable for routine analysis and detection.

Description

technical field [0001] The invention relates to the determination of soil carbohydrates, in particular to a capillary gas chromatography analysis method derived from glyconitrile acetyl esters of neutral sugars in soil. Background technique [0002] Soil carbohydrates make up 5-25% of soil organic matter [1] (Murata et al.1999. Seasonal variations in soil microbial biomass content and soil neutral sugar composition in grassland in the Japanese Temperate Zone. Applied Soil Ecology 11, 253-259.), is an important part of soil organic matter variable pool, and is an important part of soil The main source of energy for microbial processes in [2] (Martens and Loeffelmann. 2002. Improved accounting of carbohydrate carbon from plants and soils. Soil Biology & Biochemistry 34, 1393-1399). Neutral sugars are a type of soil carbohydrates that play an important role in soil aggregation [3] (Puget et al. 1999. Nature of carbohydrates associated with water-stable aggregates of two cult...

Claims

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Application Information

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IPC IPC(8): G01N1/34G01N30/00G01N31/00
Inventor 张威张旭东张明
Owner SHENYANG INST OF APPLIED ECOLOGY - CHINESE ACAD OF SCI
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