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Solid culture medium for culturing garlic sexual embryo

A solid medium and sexual technology, applied in the field of agricultural biology, can solve the problems of garlic flower abortion and failure to set seeds, etc., and achieve the effects of high success rate and good cultivation effect.

Inactive Publication Date: 2011-03-23
VEGETABLE RES INST OF SHANDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problem of garlic flower abortion and inability to set seeds, the present invention provides a solid medium for cultivating sexual embryos of garlic, which greatly improves the seedling rate and reproduction coefficient of sexual embryos of garlic

Method used

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  • Solid culture medium for culturing garlic sexual embryo
  • Solid culture medium for culturing garlic sexual embryo
  • Solid culture medium for culturing garlic sexual embryo

Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach 1

[0079] A medium for cultivating sexual embryos of garlic:

[0080](1) Inorganic substances: potassium nitrate 1700mg / L, ammonium nitrate 1550mg / L, potassium dihydrogen phosphate 150mg / L, magnesium sulfate 350mg / L, calcium chloride 400mg / L, potassium iodide 0.5mg / L, boric acid 3mg / L, Manganese sulfate 10mg / L, zinc sulfate 2mg / L, sodium molybdate 0.1mg / L, copper sulfate 0.01mg / L, cobalt chloride 0.01mg / L, iron sulfate 27.8mg / L, edetate disodium 37.3 mg / L;

[0081] (2) Organic matter: inositol 80mg / L, niacin 0.5mg / L, pyridoxine hydrochloride 0.5mg / L, thiamine hydrochloride 0.1mg / L, glycine 1mg / L, coconut milk 5%;

[0082] (3) Plant hormones: zeatin 0.05mg / L, indole acetic acid 0.05mg / L;

[0083] (4) Sucrose 15000mg / L

[0084] (5) Agar 6g / L.

[0085] Preparation method: Accurately weigh each component according to the formula, add deionized water to fully dissolve, constant volume, adjust pH to 5.8-6.0, subpackage, and sterilize.

[0086] Inorganic substances, organic substan...

Embodiment approach 2

[0088] A medium for cultivating sexual embryos of garlic:

[0089] (1) Inorganic substances: potassium nitrate 1900mg / L, ammonium nitrate 1650mg / L, potassium dihydrogen phosphate 170mg / L, magnesium sulfate 370mg / L, calcium chloride 440mg / L, potassium iodide 0.83mg / L, boric acid 6.2mg / L , manganese sulfate 22.3mg / L, zinc sulfate 8.6mg / L, sodium molybdate 0.25mg / L, copper sulfate 0.025mg / L, cobalt chloride 0.025mg / L, iron sulfate 27.8mg / L, ethylenediaminetetraacetic acid Two sodium 37.3mg / L;

[0090] (2) Organic matter: inositol 100mg / L, niacin 0.5mg / L, pyridoxine hydrochloride 0.5mg / L, thiamine hydrochloride 0.1mg / L, glycine 2mg / L, coconut milk 15%;

[0091] (3) Plant hormones: 6-benzylaminoadenine 1.0mg / L, indole acetic acid 2.0mg / L;

[0092] (4) Sucrose 30,000mg / L

[0093] (5) Agar 7g / L.

[0094] Preparation method: same as above.

Embodiment approach 3

[0096] A medium for cultivating sexual embryos of garlic:

[0097] (1) Inorganic substances: potassium nitrate 1800mg / L, ammonium nitrate 1700mg / L, potassium dihydrogen phosphate 180mg / L, magnesium sulfate 380mg / L, calcium chloride 500mg / L, potassium iodide 1.0mg / L, boric acid 7.0mg / L , Manganese Sulfate 23mg / L, Zinc Sulfate 9.0mg / L, Sodium Molybdate 0.2mg / L, Copper Sulfate 0.02mg / L, Cobalt Chloride 0.02mg / L, Iron Sulfate 27.8mg / L, EDTA Sodium 37.3mg / L;

[0098] (2) Organic matter: inositol 120mg / L, niacin 2.0mg / L, pyridoxine hydrochloride 1.5mg / L, thiamine hydrochloride 15mg / L, glycine 3mg / L, coconut milk 10%;

[0099] (3) Plant hormones: 6-benzylaminoadenine 0.5mg / L, naphthaleneacetic acid 0.25mg / L;

[0100] (4) Sucrose 20,000mg / L

[0101] (5) Agar 6.5g / L.

[0102] Preparation method: same as above.

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Abstract

The invention discloses a solid culture medium for culturing garlic epigamous embryo, belonging to the field of agricultural biotechnology. The solid culture medium includes the following components: potassium nitrate, ammonium nitrate, monopotassium phosphate, magnesium sulfate, calcium chloride, potassium iodide, boric acid, manganese sulfate, zinc sulfate, sodium molybdate, copper sulphate, cobalt chloride, ferric sulfate, sodium ethylene diamine tetracetate, cyclohexanehexol, nicotinic acid, puridoxine hydrochloride, thiamine hydrochloride, glycin, coconut cream, sucrose, 6-benzylamino adenine or zeatin, indoleacetic acid or rhodofix and agaragar. The solid culture medium for culturing garlic epigamous embryo has the advantages of good culturing effect and high success rate; based on the original foundation, the planting percent is increased by more than 30% and the propagation coefficient is improved by two times, thus greatly improving the culturing success rate of the garlic epigamous embryo or the whole plant thereof and solving the key technology of the difficult problem that the garlic can not be reproduced sexually.

Description

technical field [0001] The invention relates to a solid medium for cultivating garlic embryos, which belongs to the field of agricultural biotechnology. Background technique [0002] Garlic (A. Sativum L.) is a spicy vegetable of the Liliaceae. It is an important useful vegetable. It is not only used as a condiment in large quantities, but also developed as a health food and medicine, which can excite nerves, increase appetite, and It has certain functions of sterilization, disease prevention and treatment, and has high edible and medicinal value. [0003] However, in production, due to the abortion of garlic flowers under conventional cultivation conditions, normal seed formation and sexual hybridization cannot be carried out, so it has been possible to rely on garlic bulbs for asexual reproduction for a long time. The reproduction coefficient is very low, the amount of seeds used is large, and the production cost is also high. At the same time, the genetic background of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00C12N5/04
Inventor 孔素萍徐培文曲士松杨崇良崔德才刘冰江藤学芳
Owner VEGETABLE RES INST OF SHANDONG ACADEMY OF AGRI SCI
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