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Gene engineering preparation method of anti-Candida albicans antibacterial peptide

A Candida albicans and genetic engineering technology, which is applied in the field of preparation of anti-Candida albicans antimicrobial peptides, can solve the problems of high price of synthetic peptides and limited yield of natural antimicrobial peptides, and achieves high recovery rate, resistance to protease hydrolysis, separation Rapid effect of purification process

Inactive Publication Date: 2009-07-15
XINJIANG ACADEMY OF AGRI & RECLAMATION SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, it has become a consensus to develop antimicrobial peptides into drugs, but the output of natural antimicrobial peptides is very limited, and the price of synthetic peptides is very expensive, which will become a bottleneck in the development of new drugs. effective method

Method used

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  • Gene engineering preparation method of anti-Candida albicans antibacterial peptide
  • Gene engineering preparation method of anti-Candida albicans antibacterial peptide
  • Gene engineering preparation method of anti-Candida albicans antibacterial peptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] The nucleic acid sequence of the sheep reproductive tract antimicrobial peptide is shown in Sequence List 1,

[0051] The amino acid sequence of the sheep reproductive tract antimicrobial peptide is shown in the sequence list 2,

[0052] The natural antimicrobial peptide sequence and artificially synthesized yeast preferred codon sequence are shown in the sequence table 3,

[0053] The upstream primer sequence is shown in Sequence Listing 4,

[0054] The downstream primer sequence is shown in Sequence Listing 5,

[0055] The downstream primer sequence is shown in Sequence Listing 6,

[0056] The upstream primer sequence is shown in Sequence Listing 7,

[0057] The downstream primer sequence is shown in the sequence listing 8,

[0058] Primer concentration 0.0009mmol / L,

[0059] 1. Cloning of sheep reproductive tract antimicrobial peptide cDNA;

[0060] First, use 3 μg of total RNA (ribonucleic acid) to reverse transcribe into cDNA, and then use cDNA as a template ...

Embodiment 2

[0080] The nucleic acid sequence of the sheep reproductive tract antimicrobial peptide is shown in Sequence List 1,

[0081] The amino acid sequence of the sheep reproductive tract antimicrobial peptide is shown in the sequence list 2,

[0082] The natural antimicrobial peptide sequence and artificially synthesized yeast preferred codon sequence are shown in the sequence table 3,

[0083] The upstream primer sequence is shown in Sequence Listing 4,

[0084] The downstream primer sequence is shown in Sequence Listing 5,

[0085] The downstream primer sequence is shown in Sequence Listing 6,

[0086] The upstream primer sequence is shown in Sequence Listing 7,

[0087] The downstream primer sequence is shown in the sequence listing 8,

[0088] Primer concentration 0.0009mmol / L,

[0089] 1. Cloning of sheep reproductive tract antimicrobial peptide cDNA;

[0090] First, use 5 μg of total RNA (ribonucleic acid) to reverse transcribe into cDNA, and then use cDNA as a template ...

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Abstract

The invention relates to a gene engineering preparing method for an anti-candida albicans antibacterial peptide. The invention provides an encoding gene of sheep antibacterial peptide by using the gene engineering preparing method. A eukaryotic expression vector is constructed by using a recombinant DNA technology. Parasitifer engineering bacteria GS115 is transferred by constructing a recombinant expression vector. Expression engineering bacteria is constructed for a methanol induction expression. Recombination sheep genital tract antibacterial peptide is obtained after the expression product goes through gel filtration chromatography and reversed-phase liquid chromatography. The antibacterial peptide prepared by the invention has high thermal stability and the ability of enduring protein enzyme hydrolyses, can effectively inhibit Gram-positive bacteria, Gram-negative bacteria and epiphyte growth and also is characterized in that the separation and purification techniques are rapid and efficient with high recovery rate, etc. The invention is widely applicable to fields of veterinary drug, preservative, medical product, etc.

Description

Technical field: [0001] The invention relates to a preparation method of an anti-Candida albicans antimicrobial peptide, in particular to a genetic engineering preparation method of an anti-Candida albicans antibacterial peptide. Background technique: [0002] Antibacterial peptides (antibacterial peptides, ABP) is a general term for peptides encoded by organism genes, with small molecular weight and antibacterial activity. It is a kind of small molecular polypeptide produced by the host to resist infection by external pathogens. It is widely found in insects, plants, animals and humans. In addition to anti-bacteria, fungi and viruses, it also has anti-tumor cell effects. Therefore, antimicrobial peptides are also called peptide antibiotics. It is an important part of the body's non-specific immunity and an ancient mechanism of the organism's anti-infection immunity, so it is also called the "second defense system". Studies have shown that antimicrobial peptides have the a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/12C12N15/81C12N1/19C07K14/47C07K1/20C07K1/16C12R1/84
Inventor 薄新文库朝锋陈琛
Owner XINJIANG ACADEMY OF AGRI & RECLAMATION SCI