Ganoderma spore oil fat emulsion and quality control method and application thereof

A quality control method and technology of Ganoderma lucidum spore oil, which is applied in measuring devices, pharmaceutical formulations, emulsion delivery, etc., can solve the problems of no relevant reports on the quality control method of Ganoderma lucidum spore oil fat emulsion, and improve the body's immunity, toxicity The effect of low side effects and high precision

Inactive Publication Date: 2009-09-02
GUANGZHOU HANFANG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There have been many literature reports about the quality control method of ganoderma spore oil, and the applicant has also applied for several invention patents about the quality control method of ganoderma spore oil, but there is no relevant report about the quality control method of ganoderma spore oil fat emulsion

Method used

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  • Ganoderma spore oil fat emulsion and quality control method and application thereof
  • Ganoderma spore oil fat emulsion and quality control method and application thereof
  • Ganoderma spore oil fat emulsion and quality control method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1～6

[0040] Refinement of Ganoderma lucidum spore oil: centrifuge the Ganoderma lucidum spore oil, remove water, add active carbon accounting for 5% of the weight of the Ganoderma lucidum spore oil, stir evenly, heat to 50°C, keep warm for 30 minutes, centrifuge, fine filter, and obtain refined Ganoderma lucidum spore oil .

[0041] Preparation method: Add the emulsifier to the refined Ganoderma lucidum spore oil under the condition of nitrogen gas, and smash it at high speed until the emulsifier is completely dissolved; under the condition of constant temperature water bath 50°C and high-speed dispersion and emulsification at 12000rpm, slowly add the oil phase Mix in the isotonic agent aqueous solution until uniform; adjust the pH to 6-9 with sodium hydroxide; immediately put the prepared colostrum into the homogenizer, adjust the homogenization pressure to 60Mpa, the homogenization times to 8 times, and the homogenization temperature to 60°C. After homogenization, it is filtered ...

Embodiment 7

[0045] Example 7 Determination of 1,2-oleic acid-3-palmitic acid triglyceride and triolein in Ganoderma lucidum spore oil fat emulsion

[0046] Determination by high performance liquid chromatography:

[0047] Chromatographic conditions Use octadecyl bonded silica gel as filler; Chromatographic column: KromasilC18, 4.6mm×250mm, 5um column; Column temperature: 30°C; Mobile phase: The volume ratio of acetonitrile:isopropanol is 40:60; Light scattering detector detection; flow rate: 0.5ml / min. The number of theoretical plates is calculated according to the peak of 1,2-oleic acid-3-palmitic acid triglyceride or triglyceride trioleate, both of which are greater than 2000;

[0048] Preparation of reference substance solution: Take 1,2-oleic acid-3-palmitic acid triglyceride and 2.67 mg of triglyceride reference substance, put them in a 25ml measuring bottle, add methanol to dissolve and dilute to the mark, Make solutions containing 0.119mg and 0.107mg per 1ml, respectively, to obt...

Embodiment 8

[0053] Example 8 Content Determination of 1,2-oleic acid-3-palmitic triglyceride and triolein in Ganoderma lucidum spores

[0054] Determination by high performance liquid chromatography:

[0055] Chromatographic conditions use octadecyl bonded silica gel as a filler; chromatographic column: KromasilC18, 4.6mm×250mm, 5um column; column temperature: room temperature; mobile phase: acetonitrile: dichloromethane (59:41), evaporative light scattering detection Device detection; flow rate: 1.0ml / min. The number of theoretical plates is calculated according to the peak of 1,2-oleic acid-3-palmitic acid triglyceride or triglyceride trioleate, both of which are greater than 2000;

[0056] Preparation of reference substance solution: Take 1,2-oleic acid-3-palmitic acid triglyceride and 2.67 mg of triglyceride reference substance, put them in a 25ml measuring bottle, add methanol to dissolve and dilute to the mark, Make solutions containing 0.119mg and 0.107mg per 1ml, respectively, t...

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Abstract

The invention discloses ganoderma spore oil fat emulsion and quality control method and application thereof, comprising 2 percent to 25 percent of ganoderma spore oil, 0.5 percent to 10 percent of emulsifier, 0.2 percent to 5 percent of isotonic agent, and the rest of water; the final pH of the fat emulsion is adjusted to be six to nine. By accurately measuring the contents of 1, 2-oleic acid-3-palm acid triglyceride and glycerin trioleate as well as ergosterol in the preparation, the quality control method is used as the method and basis for controlling the product quality. By using a fingerprint, the product quality is controlled from the overall chromatographic feature appearance. The invention has clear product active ingredient and good biological activity, can be used for treating the tumor and enhancing the immune capacity of an organism, improves the survival quality of the radiotherapy and chemotherapy tumor patients and alleviates the drug toxicity after the treatment. The product is especially suitable for artery and intravenous injection; the ganoderma spore oil directly enters the human blood; and the product has the advantages of fast effect, complete absorption, high security, reliable quality and low toxicity.

Description

technical field [0001] The invention relates to the technical field of ganoderma lucidum spore oil, in particular to a fat emulsion of ganoderma lucidum spore oil and its quality control technology and medicinal application. Background technique [0002] Since the 1980s, Japan has begun to conduct basic research on the anti-tumor aspect of Ganoderma lucidum; in the 1990s, the United States, Japan and other countries have applied Ganoderma lucidum to the clinic for the treatment of cancer and AIDS (AIDS), and have received international research. wide attention of scholars. Ganoderma lucidum is the general term for the fungi of the genus Ganoderma (Ganoderma) G. lucidum. Ganoderma lucidium spores are extremely small spores ejected from the cap during the mature growth stage of Ganoderma lucidum. They are the germ cells of Ganoderma lucidum and contain all the genetically active substances of Ganoderma lucidum. Their medicinal value is also increasingly valued. The chemical ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/074A61K9/107A61K47/24A61K47/34A61K47/36A61P35/00A61P37/04G01N30/36G01N30/86
Inventor 陈路林葛发欢陈文俊曾荣华蒙庚海张爱韶赖庆水蒋兆健
Owner GUANGZHOU HANFANG PHARMA
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