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Method for preparing in-situ therapeutic substance-loaded microcapsules

A therapeutic and microcapsule technology, which is applied in the field of biomedicine and biomaterials, can solve the problem that it is difficult to meet the stable and sustained release of microcapsules, the controllable release and sustained release of drugs are difficult to maintain, and the wall layer of microcapsules collapses. and other issues, to achieve excellent biological safety and controlled drug release, which is conducive to sustained release and adjustable particle size.

Inactive Publication Date: 2010-06-09
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chinese patent CN 100488618C discloses a method for preparing ex-situ drug-carrying liquid core microcapsules by electrostatic spraying. The patent discloses using metal calcium ions as the capsule wall forming agent for alginate microcapsules, although by using malt paste Thickeners such as essence and xanthan gum and calcium ions can lock the alginate on the capsule wall, and after the microcapsules are formed, the wall membrane is strengthened by soaking in calcium chloride solution to form a semipermeable microcapsule wall. However, in the physiological fluid environment, divalent calcium ions are prone to ion exchange with monovalent cations such as potassium and sodium ions, resulting in the rapid collapse of the microcapsule wall membrane layer, and it is difficult to maintain the controlled release and sustained release of drugs
Therefore, such materials are still difficult to meet the application of stable and sustained release of microcapsules

Method used

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  • Method for preparing in-situ therapeutic substance-loaded microcapsules
  • Method for preparing in-situ therapeutic substance-loaded microcapsules
  • Method for preparing in-situ therapeutic substance-loaded microcapsules

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The weighing average molecular weight is 50000, and the degree of deacetylation is 83%. Inside the syringe of the electrospinning device;

[0031] Dissolve sodium alginate in water to prepare a sodium alginate aqueous solution with a concentration of 12g / L, then place it in a container directly below the needle tip of a hollow metal needle of a syringe, and adjust the distance between the needle tip and the liquid surface of the sodium alginate solution to be 8cm. The solution is grounded with a wire, and continuously stirred at 60 rpm, the hollow metal needle is connected to a 10kV DC voltage, and the chitosan solution in the syringe is pushed out at a speed of 0.10 ml / hour, and electrostatically sprayed, containing cyclosporine A Under the action of high-voltage electrostatic electric field, the chitosan solution instantly forms a mist of gel particles, which falls into the sodium alginate solution to form chitosan gel particles with sodium alginate as the capsule wal...

Embodiment 2

[0033] With the preparation method of Example 1, the difference is: add vascular endothelial growth factor to the chitosan solution, change the DC voltage applied by the metal needle to 15kV, and the distance between the needle tip and the grounded 12g / L sodium alginate receiving solution Change it to 12cm, make microcapsules loaded with vascular endothelial growth factor through high-voltage electrostatic spraying, and observe the morphology of this kind of microcapsules with a scanning electron microscope as attached figure 2 As shown, the microcapsules prepared by this method are nearly spherical nanoparticles.

Embodiment 3

[0035] Sodium alginate was dissolved in water to prepare a sodium alginate aqueous solution with a concentration of 30g / L, and then 0.001g of nano-titanium dioxide with a particle size of 30nm was added, mixed evenly and loaded into the syringe of the electrospinning device;

[0036] Weighing average molecular weight is 250000, and the degree of deacetylation is 86%. , adjust the distance between the needle tip and the liquid surface of the chitosan solution to be 10cm, the solution is grounded with a wire, and continuously stirred at 120 rpm, the hollow metal needle is connected to a DC voltage of 15kV, and the sodium alginate solution in the syringe is injected at a rate of 0.20 ml / hour The speed is pushed out, and electrostatic spraying is carried out. The sodium alginate solution containing nano-titanium dioxide instantly forms a mist of gel particles under the action of a high-voltage electrostatic electric field, and falls into the chitosan solution to form a microcosm wi...

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Abstract

The invention discloses a method for preparing therapeutic substance-loaded microcapsules. The method applies the high-voltage electrostatic spray principle and comprises the steps of leading therapeutic substance-loaded charged polymer solution to instantaneously form ultrafine gel particles under the high-voltage electrostatic action, leading a mist of the particles to be received by polymer solution charged with opposite charges, leading a surface layer of the gel particles to be wrapped by a polymer gel shell layer of the opposite charges, forming structures of capsule cores and capsule walls, further filtering by a microporous membrane, and obtaining the therapeutic substance-loaded microcapsules. Microstructures and scale of the capsule cores and the capsule walls can be precisely controlled through voltage, solution concentration and distance from a jet orifice to receiving solution, and therapeutic substances are bound by the capsule cores and the capsule walls, thereby forming controlled release and improving therapeutic efficacy. The method has the advantages of simple and feasible process and good repeatability, the whole process of the preparation process is clean and pollution-free, the provided in-situ therapeutic substance-loaded microcapsules do not need the complex post-processing, the particle size is controllable, and the release rate of the therapeutic substances is convenient to control, thereby having good application prospect.

Description

technical field [0001] The invention relates to a method for preparing microcapsules, in particular to a method for preparing microcapsules loaded with therapeutic substances in situ, and belongs to the technical fields of biomedicine and biomaterials. Background technique [0002] Microcapsules refer to the use of natural or synthetic polymer materials to wrap dispersed solids, liquids, and even gaseous substances to form tiny particles with semi-permeable or sealed capsules. So far, the effective microencapsulation of active substances such as drugs, proteins, growth factors, vitamins, spices, antibacterial ions, nanoparticles, etc., and even living cells has been used in many fields such as disease treatment and prevention, functional food, cosmetics, industrial separation, etc. etc. are more and more widely used, and become one of the high-tech materials that will be researched and developed in the 21st century. [0003] At present, the preparation methods of microcapsu...

Claims

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Application Information

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IPC IPC(8): A61K9/50A61K45/00B01J13/02
Inventor 苟中入叶娟王嫦君苏鹏
Owner ZHEJIANG UNIV
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