Polyurethane material capable of dissolving plasma clot in high efficiency and preparation thereof

A polyurethane material and polyurethane technology, applied in the field of biomedical functional polymer materials, can solve the problems of limiting the grafting density of bioactive molecules, it is difficult to achieve high-density grafting, and the anticoagulation process cannot be achieved.

Active Publication Date: 2011-01-12
JIANGSU BIOSURF BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although PEG has a good ability to repel non-specific protein adsorption, in the environment where oxygen and excessive metal ions exist, PEG is easily oxidized and decomposed, so long-term application in physiological environment will lead to the formation of biofilm and limit its use. Application in human implant materials
In addition, it is often difficult to achieve high-density grafting by chemically grafting PEG on the surface of materials, which limits the grafting density of bioactive molecules to a large extent, so that high-efficiency anticoagulation cannot be achieved.

Method used

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  • Polyurethane material capable of dissolving plasma clot in high efficiency and preparation thereof
  • Polyurethane material capable of dissolving plasma clot in high efficiency and preparation thereof
  • Polyurethane material capable of dissolving plasma clot in high efficiency and preparation thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0032] (1) Polyurethane surface graft copolymerization PHEMA

[0033]Put 0.98g of potassium thiocyanate and 40mL of anhydrous acetonitrile in a 100mL reaction flask and stir to dissolve. 0.82 mL of acryloyl chloride was slowly dropped into the reaction bottle, and stirred at room temperature for 12 hours. The precipitate was removed by filtration, and the filtrate was directly used in the next reaction. Put 60 polyurethane diaphragms (thickness 0.5mm) with a diameter of about 7mm in a reaction flask containing 30mL of the above filtrate, add 0.75g of triethylamine to the reaction flask, stir and react at 65°C for 2-12 hours, and then put the polyurethane The diaphragm is taken out, washed with acetonitrile and dried to obtain a polyurethane diaphragm with carbon-carbon double bonds on the surface. Place 0.082g of azobisisobutyronitrile, 6.5g of HEMA, 40mL of methanol and 60 pieces of polyurethane diaphragms with carbon-carbon double bonds on the surface in a 50mL reaction bo...

Embodiment 2

[0038] The lysine surface prepared according to the method of Example 1 was tested for the adsorption of isotope-labeled fibrinogen to reflect its ability to reject non-specific protein adsorption. isotope by the ICl method 125 I was labeled on the tyrosine residue of fibrinogen, and the labeled and unlabeled fibrinogen solutions were mixed according to the mass ratio of fibrinogen of 1:19, and the modified and unmodified polyurethane membranes were placed in a static place. In this solution for 3 hours, washed three times with PBS or TBS buffer solution with a pH of 7.4, and finally read the amount of radioactivity on the membrane through a gamma counter and converted it into the mass of protein, and finally obtained figure 1 .

Embodiment 3

[0040] The ability of the lysine surface prepared according to the method of Example 1 to reject non-specific protein adsorption and selectively bind to plasminogen in plasma was tested by Western blot technique. First, the modified and unmodified polyurethane membranes were soaked in plasma for 3 hours, washed three times with PBS or TBS buffer solution with a pH of 7.4, and then washed with 2% (w / v) sodium dodecyl sulfate (SDS) solution The protein adsorbed on the surface of the membrane is eluted. Then, the protein eluate was electrophoresed on a 12% SDS-PAGE gel, and then the protein on the gel was transferred to a PVDF membrane, and the PVDF membrane was blocked with 5% skimmed milk powder, and then combined with the studied The primary antibody of the target protein and the secondary antibody labeled with alkaline phosphatase (fibrinogen and plasminogen were studied in this example), and finally by acting on the substrate 5-bromo-4-chloro-3-ind Indophosphoric acid and n...

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Abstract

The invention relates to the field of biomedical functional polymer materials, in particular to a method for preparing a polyurethane material with the function of dissolving plasma clot in high efficiency. The method comprises the following steps: firstly, using a polyurethane material as a substrate, and using hydrophilic polymer serving as an inert spacer arm to modify the surface of the polyurethane substrate; then, activating the tail end of a lateral chain of the inert spacer arm, and combining bioactive molecule epsilon-lysine to obtain the polyurethane material with the function of dissolving plasma clot, wherein the hydrophilic polymer is polyhydroxyethyl methacrylate. By utilizing the bioinert and comb structure of PHEMA, specific binding of plasminogen is improved while non-specific protein adsorption is rejected, and the lysine molecule can be grafted in high density so as to improve the efficiency of binding the plasminogen, thus achieving the aim of dissolving the plasma clot in high efficiency.

Description

technical field [0001] The invention relates to the field of biomedical functional polymer materials, in particular to a preparation method of a polyurethane material with the function of efficiently dissolving primary thrombus. Background technique [0002] Cardiovascular disease has become one of the biggest killers threatening human health, and the materials and devices used for diagnosis and treatment of cardiovascular disease are inevitably in contact with blood. Therefore, biomedical materials in contact with blood have very broad application prospects and a large market demand. Treatment equipment (hemodialysis, extracorporeal circulation), etc. However, the problem of thrombus caused by the surface of the material has always restricted the development of this type of material. Using biologically inert polymer chains as spacer arms to immobilize bioactive molecules with binding anticoagulant factors on the surface of materials is an effective way to solve the problem...

Claims

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Application Information

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IPC IPC(8): C08J7/12C08J7/16C08L75/04A61L33/14A61L33/06B32B27/08B32B27/40
Inventor 陈红李丹王莎莎
Owner JIANGSU BIOSURF BIOTECH CO LTD
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