Method for separating impurities in solution by chemically modified solid nanopore arrays

Abstract

The invention relates to a method for separating impurities in a solution by chemically modified solid nanopore arrays. The area of the solid nanopore arrays is not less than 250 square micrometers. The diameter of a single nanopore is 1-200nm. Pore density is not less than 1 pore / square micrometer. The inner wall of pores is modified with one component or a mixture of more than two components ata random ratio, wherein the components are selected from the group consisting of 6-28bp nucleic acid molecule, antigen molecule, 60-200kDa antibody molecule, peptide molecule containing 6-50 amino acid and silane molecule. When 1-50 microliters of a solution which contains impurity molecule at the concentration of 1-10 nmol.ml<-1> or impurity particles of less than 1microgram / ml passes through the solid nanopore arrays, the impurity molecule or particles are intercepted by the chemically modified nanopore arrays. The structural formula of the silane molecule is Y(CH2)nSiXmZ3-m, wherein n is 0-3.

Description

technical field [0001] The invention relates to a method for separating impurities in a solution by chemically modifying a solid nanopore array. Background technique [0002] Nanopore refers to a hole on the substrate with a thickness of nanometer to micrometer, with a diameter of 1 to hundreds of nanometers, and penetrates both sides of the substrate. Nanopores can be divided into two categories in terms of preparation methods. The natural nanopores formed by biomolecules are called biological nanopores, and the nanopores obtained by people using micro-nano processing technology are called solid-state nanopores. The application of biological nanopores originated in 1996, when Kasianowicz and his colleagues reported for the first time that single-stranded DNA or RNA passed through α-hemolysin nanopores under the action of an electric field, and obtained a blocking current (Blockade Current) when molecules passed through the pores. The phenomenon of blocking current amplitu...

AI Technical Summary

Problems solved by technology

These technologies have their own scope of application, but their main disadvantages are: 1) the separation process itself will introduce new impurities; 2) the degree of separation is limited, and it is impossible to achieve absolute separation of single-molecule impurities; these shortcomings may affect some specific applications. cause adverse consequences

For example, during DNA cloning and library preparation, if the impurity DNA molecule cannot be completely removed, the impurity will be amplified simultaneously with the target sequence in a large amount during the amplification process, and the purity and reliability of the prepared clone or library will decrease , the subsequent use of these samples, such as DNA sequencing, will cause a decrease in accuracy and difficulties in data post-processing, etc.