Method for preparing high quality Antarctic krill oil rich in phosphatidylserine containing polyunsaturated double-bonded fatty acyl

Abstract

The invention relates to a method for preparing a high quality Antarctic krill oil rich in phosphatidylserine containing polyunsaturated double-bonded fatty acyl. The method comprises the following steps: 1) carrying out solvent extraction on existing Antarctic krill oil to obtain phosphatidylcholine and oil containing vitamin A, vitamin E and astaxanthin; 2) preparing an enzyme catalysis reaction system, which is an aqueous phase reaction system; 3) adding the reaction system into a reactor, adding phospholipase D and carrying out mechanical agitation continuously; 4) extracting obtained reaction repeatedly and carrying out freeze drying on filtered solid to obtain phosphatidylserine rich in polyunsaturated double-bonded fatty acyl; 5) mixing the obtained phosphatidylserine rich in polyunsaturated double-bonded fatty acyl with the oil obtained in the step 1) to obtain the high quality Antarctic krill oil. The invention also provides a method for preparing phosphatidylcholine rich in polyunsaturated double-bonded fatty acyl from existing Antarctic krill oil and a method for preparing phosphatidylserine rich in polyunsaturated double-bonded fatty acyl.

Description

Technical field: [0001] The invention relates to a method for preparing high-quality Antarctic krill oil rich in phosphatidylserine with polyunsaturated double bond fatty acyl groups, and in particular to a method that uses phosphatidylcholine from Antarctic krill as a raw material and is catalyzed by an enzyme A method for preparing phosphatidylserine rich in polyunsaturated double bond fatty acyl groups and compounding it to obtain high-quality Antarctic krill oil. Background technique: [0002] In the prior art, there are many methods for preparing phosphatidylserine, among which extraction methods and enzyme conversion methods are the main methods. The extraction method is to extract phosphatidylserine from animal phospholipids, mainly using animal brains as raw materials. Patent CN1583766A is to extract phosphatidylserine from animal brain. However, due to mad cow disease and other reasons, the safety of its products has been suspected and has now been eliminated. In rece...

AI Technical Summary

Problems solved by technology

In recent years, the enzymatic preparation of phosphatidylserine from soybean lecithin has been widely used, such as patent CN101230365A, but the product formed by this patent does not contain phosphatidylserine with polyunsaturated double bond long-chain fatty acyl groups

Patent CN101818179A synthesizes phosphatidylserine containing polyunsaturated double-bond fatty acyl groups from ordinary lecithin by two-step enzymatic method. Phospholipids in ordinary lecithin do not contain Omega-3 polyunsaturated fatty acyl groups. The reaction is to use phospholipase A1, A2 or lipase to catalyze fatty acids containing polyunsaturated double bonds and phosphatidylcholine in lecithin to generate phosphatidylcholine containing polyunsaturated double bond fatty acyl groups, and then use phospholipase D to catalyze The reaction of serine and the phosphatidylcholine containing polyunsaturated double-bond fatty acyl finally generates the phosphatidylserine containing polyunsaturated double-bond fatty acyl. The weak point of this invention is: raw material lecithin and polyunsaturated fatty acid It is a complex mixture in which the effective content of phosphatidylcholine and polyunsaturated fatty acid is not high. In the case of no high concentration, the effective content of these two raw materials is generally not more than 50%, while in phospholipase Under the catalysis of A2 or lipase specific to SN2 position, the probability of generating the target product SN2 position is phosphatidylcholine containing polyunsaturated double bond fatty acyl group is very low, which leads to the polyunsaturated double bond contained in the final product. The concentration of phosphatidylserine with bonded fatty acyl groups is lower, which is not conducive to the quality of the final product. If the active ingredient concentration of phosphatidylserine with polyunsaturated double bond fatty acyl groups in the product is to be increased, the production cost will be seriously increased

Patent CN100402656C uses phospholipid extracted from cod liver as raw material, patent CN101157946A uses phospholipid extracted from squid as raw material, adopts phospholipase D enzymatic method to catalyze phosphatidylcholine in cod liver or phosphatidylcholine in squid and L-serine reacts to prepare phosphatidylserine rich in polyunsaturated double-bond fatty acyl groups. Among them, phosphatidylcholine rich in polyunsaturated double-bond fatty acyl groups in cod liver phospholipid and squid phospholipid is the preparation of polyunsaturated double-bond fatty acyl-containing phosphatidylcholine. It is an excellent raw material for phosphatidylserine with fatty acyl groups, but the sources of these two raw materials are limited, which is not conducive to large-scale production

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