Nitrilase and gene and application thereof

A technology of nitrilase and hydrolysis reaction, applied in the field of bioengineering, can solve the problems of low yield and poor tolerance, and achieve the effects of high yield, high ee value, and good substrate tolerance
CN102533705AInactive Publication Date: 2012-07-04EAST CHINA UNIV OF SCI & TECH
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
EAST CHINA UNIV OF SCI & TECH
Publication Date
2012-07-04
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a new nitrilase and gene thereof, a recombinant expression vector and a recombinant expression transformant containing the gene, a method for preparing the recombinant nitrilase or microbial cell containing the recombinant nitrilase by use of the recombinant expression transformant, and application of the microbial cell in dehydrating ortho-chlorine mandelonitrile or other analogues and producing chiral ortho-chlorine mandelonitrile or other analogues. The recombinant nitrilase disclosed by the invention comes from Labrenzia aggregate and can be used as a catalyst for dehydrating and splitting ortho-chlorine mandelonitrile or other analogues; and the recombinant nitrilase has the advantages of high catalysis efficiency, strong enantioselectivity, mild reaction conditions, environmental friendliness and the like.
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Description

technical field

[0001] The invention belongs to the technical field of bioengineering, and particularly relates to a new nitrilase and its gene, a recombinant expression vector and a recombinant expression transformant containing the gene, a method for preparing a recombinant enzyme using a recombinant expression transformant, and the recombinant nitrilase The application of the enzyme in hydrolyzing o-chloromandelonitrile or its structural analogues to prepare (R)-o-chloromandelic acid or its structural analogues. Background technique

[0002] (R)-o-chloromandelic acid is an important chiral intermediate of the anticoagulant drug clopidogrel. And its analogs are an important class of chiral synthetic building blocks, which are widely used in the synthesis of various drugs. Such as cephalosporin semi-synthetic antibiotics, important intermediates of anti-tumor and anti-aging drugs; it can also be used as a chiral resolution reagent for the resolution of other chiral drugs o...

Claims

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