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Bacillus cereus AR156 wettable powders as well as preparation and application thereof

A technology of Bacillus cereus and wettable powder, applied in the field of Bacillus cereus AR156 wettable powder and its preparation, can solve the problems of inconvenient use, high transportation cost and easy pollution, and achieve high transportation cost, low cost, The effect of improving shelf life

Inactive Publication Date: 2012-07-18
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a wettable powder of Bacillus cereus AR156 for the defects of high transportation cost, inconvenient use and easy pollution caused by using AR156 fermented liquid directly as a preparation

Method used

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  • Bacillus cereus AR156 wettable powders as well as preparation and application thereof
  • Bacillus cereus AR156 wettable powders as well as preparation and application thereof
  • Bacillus cereus AR156 wettable powders as well as preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] 1.1 Compatibility testing of carriers and dispersants with Bacillus cereus AR156

[0037] The carrier (white carbon black, corn flour, bentonite, kaolin, attapulgite, soybean powder, talcum powder, pottery clay, fineness all passed through a 325 mesh sieve), dispersant (starch, xanthan gum, gum arabic, lignin sulfonate) sodium carboxymethyl cellulose, ammonium dodecylbenzenesulfonate, polyethylene glycol) were added into LB culture solution (250ml Erlenmeyer flask, liquid volume 100ml) according to the ratio of 5% and mixed evenly, after sterilization Insert 1ml of AR156 seed solution, place in a shaker, and incubate at 28°C and 180rpm for 24h. The culture fluid without loading body and dispersant was used as the control, and each treatment was repeated 3 times. The contents of bacteria and spores in the fermentation broth were determined by plate colony counting method. Carriers and adjuvants that have a greater impact on the amount of sporulation (compared with the c...

Embodiment 2

[0055] 2.1 Preparation of AR156 fermentation concentrate

[0056] A single colony of AR156 (CGMCC NO.1929) was picked, placed in a test tube filled with LB culture solution, cultivated at 28° C., 200 rpm for 24 hours, and used as a seed solution. According to the inoculum size of 5%, the seed solution was inoculated on the optimized medium (maltose 0.25%, corn flour 0.5%, soybean flour 0.5%, tryptone 0.5%, CaCl 2 2H 2 O 0.05%, MnSO 4 ·H 2 O 0.05%, K 2 HPO 4 0.1%, filtered after boiling, adjusted to pH 7.0), 28°C, 200rpm, cultivated for 48h, and the spore content was about 1×10 9 The CFU / mL fermented liquid was concentrated to 25% of the original volume (that is, the content of the spores was increased by 4 times) to obtain a concentrated fermented liquid for subsequent use.

[0057] Detection method of spore content: plate colony counting method: take 100 μL of AR156 fermentation broth, add it to 900 μL sterilized water, shake and mix, draw 100 μL from it and add it to 9...

Embodiment 3

[0063] Example 3 AR156 wettable powder storage conditions and shelf life detection

[0064] 3.1 The influence of temperature on the shelf life of preparations

[0065]Prepare bacterial agents according to the above method, and store them at 4°C, 25°C and 37°C respectively. Samples were taken at 0, 3, 7, 15, and 30d to detect the content of active ingredients. Each treatment was repeated 3 times.

[0066] After AR156 wettable powder was placed under different storage conditions for a certain period of time, the spore content would decrease to varying degrees. The temperature is positively correlated with the rate of decline. Low temperature conditions can inhibit the germination of spores and keep the spores in a dormant state. Therefore, after the preparation is stored at 4°C for 30 days, the spore content can still maintain a high level ( Figure 6 , A).

[0067] 3.2 The effect of initial pH on the shelf life of preparations

[0068] Prepare the bacterial agent according...

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Abstract

The invention discloses bacillus cereus AR156 (Analytical Reagent156) wettable powders as well as the preparation and application thereof. The bacillus cereus AR156 wettable powders comprise lactose, white carbon black, arabic gum, monopotassium phosphate and bacillus cereus AR156 spores. Bacillus cereus AR156 fermented concentrated solution is taken and mixed with lactose; mixed liquor is added in white carbon black with 325 meshes, and the mixture is stirred fully and is then put on a sterile tray; the mixture is dried for 11 to 13 hours under the temperature ranging from 60 to 70 DEG C, and the final moisture content can reach 20 to 30 percent; dried products are crushed, arabic gum is added during the crushing process, and then crushing is carried out again; and monosodium phosphate is added so as to adjust the pH value until the pH value reaches 7.0, so that the AR156 wettable powders are obtained. The AR156 wettable powders can successfully solve the negative influence that is caused when bacillus fermentation liquor is taken as preparation for using directly, and can achieve stable and reliable greenhouse control to tomato bacterial wilt.

Description

technical field [0001] The invention belongs to the field of pesticide preparations, and relates to a Bacillus cereus AR156 wettable powder and a preparation method and application thereof. Background technique [0002] Bacillus spp. is the dominant species of soil and plant microecology. As beneficial microorganisms in the rhizosphere of plants, they play a variety of beneficial roles in the prevention and control of plant diseases by secreting antibiotics and growth competition. The biogenic pesticides developed with them as active ingredients have become ideal substitutes for current chemical pesticides because they meet the needs of environmental protection, food safety and human health, and obtaining Bacillus preparations with stable properties and easy transportation and storage is a smooth industry. base of transformation. [0003] Bacillus can produce heat-resistant and stress-resistant spores in the later stage of reproduction. This feature can minimize the death o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/02A01N25/14A01N25/08A01P3/00
Inventor 郭坚华庄振国李咏梅
Owner NANJING AGRICULTURAL UNIVERSITY
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