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Active lactic acid bacteria capsules with treatment and prevention effect on vaginitis

A technology for active lactic acid bacteria and vaginitis, which is applied in the field of active lactic acid bacteria capsules that have a therapeutic and preventive effect on vaginitis, can solve the problems that the safety is not as good as that of lactic acid bacteria, endocarditis, diarrhea and fever, and is not very suitable for colonization. Improves overall mucosal immunity, improves vaginitis, reduces risk of gut bacteria

Inactive Publication Date: 2012-10-17
郑州金森生物科技工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The invention with application number 200710193035.1 relates to a capsule of Lactobacillus delbrueckii, wherein the lactobacillus is Lactobacillus delbrueckii, according to literature reports, Lactobacillus delbrueckii in The isolation rate in the vagina of healthy women is low, less than 1%. This kind of lactobacillus is more common in the intestinal tract, and from its origin, it will not be very suitable for colonization in the vagina of most people
The invention with the application number of 200910024226.4 relates to a capsule of live Streptococcus enterococcus, which is a kind of enterococcus, although it is also a lactic acid bacteria, but its isolation rate in the vagina of healthy women is low, and it is more common in the intestinal tract, and according to It has been reported in the literature that Streptococcus enterica is an opportunistic pathogen. Although it does not cure the disease under normal conditions, it may cause urinary tract infection, suppurative abdominal infection, sepsis, endocarditis, diarrhea and fever, etc., and there are potential dangers. Its safety is not as good as Lactobacillus. Both the International Probiotics Association and the World Health Organization (WHO) recommend that Enterococci should not be used in probiotics
In addition, because the ideas of these two inventions are too narrow, they are only limited to adjusting the vaginal flora, and do not consider the human body as a whole.

Method used

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  • Active lactic acid bacteria capsules with treatment and prevention effect on vaginitis
  • Active lactic acid bacteria capsules with treatment and prevention effect on vaginitis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1~6

[0030] The first step of the present invention is to establish a corresponding lactic acid bacteria seed bank.

[0031] Establishment of Lactobacillus crispatus Seed Bank

[0032] 1. Collect the vaginal secretions of women without a history of vaginitis, serially dilute the secretions with normal saline and put them in a refrigerator at 4°C for use, and keep them for no more than 3 days.

[0033] 2. Configure standard MRS solid medium, autoclave at 121°C and pour it into a 10 cm petri dish, and put it in a 4°C refrigerator after cooling and solidification.

[0034] 3. Pipet 200 microliters of vaginal secretion diluent into the MRS plate, spread it evenly with a spreading rod and place it in an anaerobic culture tank, and incubate at 37°C for 48 hours.

[0035] 4. Pick a single bacterial clone, perform gram staining, expand the gram-positive bacillus clone, and use conventional biochemical and molecular biology techniques for identification of bacterial species to identify the bacterial ...

Embodiment 1

[0044] Preparation of active lactic acid bacteria capsule with curative and preventive effects on vaginitis

[0045] 1. Isolate Lactobacillus crispatus from vaginal secretions and establish Lactobacillus crispatus seed bank

[0046] 1. Collect the vaginal secretions of women without a history of vaginitis, serially dilute the secretions with normal saline and put them in a refrigerator at 4°C for use, and keep them for no more than 3 days.

[0047] 2. Configure standard MRS solid medium, autoclave at 121°C and pour it into a 10 cm petri dish, and put it in a 4°C refrigerator after cooling and solidification.

[0048] 3. Pipet 200 microliters of vaginal secretion diluent into the MRS plate, spread it evenly with a spreading rod and place it in an anaerobic culture tank, and incubate at 37°C for 48 hours.

[0049] 4. Pick a single bacterial clone, perform gram staining, expand the gram-positive bacillus clone, and use conventional biochemical and molecular biology techniques for identific...

Embodiment 2~6

[0065] 1. Preparation of Lactobacillus crispatus powder

[0066] The preparation process is the same as in Example 1. The total number of viable bacteria of the Lactobacillus crispatus powder prepared is 1×10 6 To 1×10 12 Between pieces / g.

[0067] 2. Preparation of Lactobacillus rhamnosus powder, Lactobacillus acidophilus powder, Lactobacillus fermentum powder and Bifidobacterium powder

[0068] Using a method similar to the preparation of the Lactobacillus rhamnosus seed bank in Example 1, the Lactobacillus rhamnosus working seed bank, the Lactobacillus acidophilus working seed bank, the Lactobacillus fermentum working seed bank and the bifidobacterium working seed bank were established respectively , And then refer to the preparation steps of Lactobacillus crispatus in Example 1, respectively prepare Lactobacillus rhamnosus, Lactobacillus acidophilus, Lactobacillus fermentum and Bifidobacterium bacterial powder, the prepared rat The total number of live bacteria of Lactobacillus ...

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Abstract

The invention relates to active lactic acid bacteria capsules with a treatment and prevention effect on vaginitis. The lactic acid bacteria capsules capable of simultaneously adjusting dysbacteriosis of intestinal tracts and vagina and emphasizing treatment and prevention of the vaginitis are developed for achieving comprehensive effects. According to the technical scheme, excipient and lactic acid bacteria mixed bacteria powder are wrapped in the active lactic acid bacteria capsules with the treatment and prevention effect on vaginitis, and the lactic acid bacteria mixed bacteria powder comprises lactobacillus crispatus powder and other lactic acid bacteria subspecies powder with an effect of adjusting the intestinal flora. The active lactic acid bacteria capsules can be used for treating the vaginitis by adopting oral administration and intra-vaginal use; the oral lactobacillus can effectively improve the immunity of mucosa by stimulating the immune response of the intestinal mucosa, adjust the overall function state of a human body and well treat the vaginitis; and a large quantity of lactobacillus proliferated in the intestinal tract can inhibit growth of intestinal bacteria and reduce the risk of intestinal bacteria infection in vagina.

Description

technical field [0001] The invention belongs to a medicine capable of treating and preventing vaginitis, and in particular relates to an active lactic acid bacteria capsule capable of treating and preventing vaginitis. Background technique [0002] Vaginitis is a very common vaginal infectious disease. In recent years, with the development of microecology, the medical community has gradually realized that the balance of vaginal microecology plays an extremely important role in the pathogenesis of female vaginitis. Vaginal microecology The point of view is that there is a micro-ecological system composed of hundreds of millions of bacteria in the vagina of healthy women, of which more than 90% of the bacteria are vaginal lactobacilli. They are the main performers of vaginal defense functions. Vaginal lactobacillus can pass Produce lactic acid, maintain an acidic environment, compete for adhesion, enhance vaginal mucosal immunity and other mechanisms to resist the invasion of ...

Claims

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Application Information

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IPC IPC(8): A61K35/74A61P15/02A61K35/744
Inventor 刘景伟胡军
Owner 郑州金森生物科技工程有限公司
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