Temperature-sensitive block copolymer and its hydrogel as well as application

A technology of block copolymers and copolymers, applied in the fields of application, animal cells, food science, etc., to achieve the effects of high purity, easy operation and controllable properties

Inactive Publication Date: 2012-10-17
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, block copolymers with poly(2-alkyl-2-oxazoline) (PAOz), such as poly(2-ethyl-2-oxazoli

Method used

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  • Temperature-sensitive block copolymer and its hydrogel as well as application
  • Temperature-sensitive block copolymer and its hydrogel as well as application
  • Temperature-sensitive block copolymer and its hydrogel as well as application

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0032] Example 1: Synthesis of poly(2-ethyl-2-oxazoline)-polylactic acid-poly(2-ethyl-2-oxazoline) (PEOz1000-PLA2300-PEOz1000)

[0033] In a three-necked flask equipped with a stirrer, add 2-ethyl-2-oxazoline (10g, 150mmol), acetonitrile (40mL), and methyl p-toluenesulfonate (0.47g) at an oil bath temperature of 80°C. Under nitrogen atmosphere, the reaction was stirred for 30h. After cooling, the methanol solution of KOH was added, and the reaction was continued for 4 hours. The solvent was removed, the residue was dissolved in THF, passed through a silica gel column, and the effluent was poured into excess cold ether for precipitation, suction filtration, and vacuum drying for 12 hours. The obtained PEOz-OH powder (4g) was dissolved in chlorobenzene (80mL), D,L-lactide (6.3g) and stannous octoate (0.63g) were added under nitrogen atmosphere, and reacted at 140°C for 24h. The reaction solution was poured into excess ether for precipitation, filtered, and vacuum dried at room te...

Example Embodiment

[0034] Example 2: Synthesis of poly(2-methyl-2-oxazoline)-polycaprolactone-poly(2-methyl-2-oxazoline) (PMOz1200-PCL2000-PMOz1200)

[0035] In a three-necked flask equipped with a stirrer, add 2-methyl-2-oxazoline (15g), acetonitrile (40mL), and methyl p-toluenesulfonate (0.47g). At an oil bath temperature of 80°C, The reaction was stirred under nitrogen atmosphere for 30h. After cooling, the methanol solution of KOH was added, and the reaction was continued for 4 hours. The solvent was removed, the residue was dissolved in THF, passed through a silica gel column, and the effluent was poured into excess cold ether for precipitation, suction filtration, and vacuum drying for 12 hours. The obtained PMOz-OH powder (0.47g) was dissolved in chlorobenzene (40mL), caprolactone (0.47g) and stannous octoate (0.47g) were added under nitrogen atmosphere, and reacted at 140°C for 24h. The reaction solution was poured into excess ether for precipitation, filtered, and vacuum dried at room te...

Example Embodiment

[0036] Example 3: Synthesis of polylactic acid-poly(2-ethyl-2-oxazoline)-polylactic acid (PLA900-PEOz3000-PLA900)

[0037] Dissolve 2-ethyl-2-oxazoline (9.9g) and 1,4-dibromo-2-butene (420mg) in acetone (40mL), and under nitrogen atmosphere at 100°C (stir and reflux for 20h. Cool. After reaching room temperature, add 0.1 mol / L KOH methanol solution (40 mL) to the reaction flask, and continue the reaction for 4 hours. Pass through a silica gel column, pour the effluent into excess cold ether for precipitation, suction filtration, and vacuum dry for 24 hours. HO-PEOz-OH powder (2g) was dissolved in chlorobenzene (20mL), D,L-lactide (0.58g) and stannous octoate (30mg) were added under nitrogen atmosphere, and reacted at 140°C for 24h. The reaction solution was poured into excess ether for precipitation, filtered, and vacuum dried at room temperature for 12 hours to obtain the triblock copolymer PLA900-PEOz3000-PLA900 of the present invention.

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Abstract

The invention relates to a block copolymer and its hydrogel as well as application. The block copolymer of the invention is a temperature-sensitive block copolymer with a solution-gel transition characteristic. Specifically, the block copolymer of the invention is a BAB or ABA-type triblock copolymer composed of a poly(2-alkyl-2-oxazoline) (PAOz) block B and a polyester (PE) block A, or a copolymer containing the triblock copolymers. The aqueous solution of the block copolymer undergoes solution-gel phase transition along with temperature rise, and the phase transition temperature is 20-80DEG C. The block copolymer has good biocompatibility and biodegradability. The copolymer provided in the invention can provide a suitable drug delivery system for controlled release of stereotactic injection preparations and drugs, and also can provide a gel matrix and an effective means for enzyme immobilization, cell culture, tissue engineering, and vascular thrombosis, etc., as well as provides an appropriate material and technique for gelation of various aqueous solutions or aqueous humor systems in food, health care products and other fields. The method involved in the invention has the advantages of simple operation, high product yield, high purity, controllable property, and suitability for large-scale production application.

Description

technical field [0001] The present invention relates to a temperature-sensitive block copolymer, its hydrogel and its use. Background technique [0002] The aqueous solution of some polymer materials undergoes a phase transition as the temperature increases, from a free-flowing liquid state at room temperature to a non-flowing solid or semi-solid state at a higher temperature (such as physiological temperature), forming a so-called polymer Hydrogel - temperature sensitive in situ gel. In recent years, thermosensitive gels have attracted much attention in the fields of biology, medicine, and pharmacy due to their good biocompatibility, strong affinity with the drug site, especially mucosal tissue, long residence time, and good controlled release performance. The gelation process does not involve any organic solvent, and the curing molding is not limited by the geometric shape of the diseased part. The temperature-sensitive polymer hydrogel is an ideal drug controlled release...

Claims

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Application Information

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IPC IPC(8): C08G81/00C08G73/06C08G63/685C08J3/075C08L87/00A61K47/34A61L27/18A61L31/06C12N11/08C12N5/071A23L1/30
Inventor 刘艳李馨儒王小宁周艳霞
Owner PEKING UNIV
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