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Production method of soybean isoflavone glycoside

A technology of isoflavone aglycone and soybean isoflavone, which is applied in the field of preparing high-purity soybean isoflavone aglycone, can solve the problems of toxic organic solvent residue, low extraction rate, time and energy consumption, etc., and achieves easy industrial production and improved extraction The effect of speed and reduction of production cost

Inactive Publication Date: 2013-03-27
TIANJIN UNIV
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Problems solved by technology

These commonly used extraction agents still have some problems, such as weakly alkaline aqueous solution, the content of impurities such as sugars and proteins in the extract increases, which increases the difficulty of the subsequent separation process, the resulting product has low purity, high environmental protection costs, and is not suitable for industrial production; if If toxic solvents such as methanol are used in the process, the residues in the product will limit the application of the product; considering various factors, most companies use ethanol aqueous solution to extract soybean isoflavones in soybean meal, but the extraction rate is low, time-consuming and energy-consuming (2) Supercritical fluid extraction method (patent publication number CN1570125A) Although this method has the advantages of high extraction rate, good product purity, simple process, and no organic solvent residue, it has high requirements for equipment, complex devices, and high cost; (3) Ultrasonic extraction method (patent publication number CN101845077A) and microwave extraction method: can significantly improve the extraction efficiency, but they are mostly used as auxiliary extraction methods, which consume a lot of energy and cost, which hinders further promotion and application
[0005] The processes for converting soybean isoflavone glucosides into aglycones mainly include acid hydrolysis (patent publication number CN101085768B) and alkaline hydrolysis. Among them, the soybean isoflavone aglycone obtained by alkaline hydrolysis is unstable and easy to degrade. At present, acid hydrolysis is mostly used Soybean isoflavone aglycone is produced, but acid hydrolysis requires higher temperature and strong acid, which requires higher production equipment; enzymatic hydrolysis conditions are mild, and weak acid buffer solution is often used, and soybean isoflavone aglycone is not easy to denature, but β- The price of glucosidase is high and the economic benefit is low
[0006] The methods for refining soybean isoflavones mainly include the following: ultrafiltration membrane method (patent publication number CN1733926C), adsorption method, column chromatography, high performance liquid chromatography, petroleum ether recrystallization method (patent publication number CN1824661C) and solvent Extraction and other methods, most of these methods have defects such as high cost, toxic organic solvent residues, and food safety hazards

Method used

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  • Production method of soybean isoflavone glycoside
  • Production method of soybean isoflavone glycoside
  • Production method of soybean isoflavone glycoside

Examples

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Effect test

Embodiment 1

[0025] (1) Alkaline ethanol aqueous solution extraction

[0026] Crush the defatted soybean meal and pass through a 60-80 mesh sieve. Take 10g 60~80 mesh defatted soybean meal powder, dissolve in 100ml 70% ethanol solution, Ca(OH) 2 Adjust the pH to 9.0, place the system in a constant temperature water bath at 70°C for extraction, stir and extract for 2 hours, after the extraction is complete, filter with suction to remove the filter residue, and take the supernatant for later use.

[0027] (2) Enzymatic hydrolysis of soybean isoflavones

[0028] Use HCl with a mass fraction of 15% to adjust the pH of the supernatant obtained in step (1) to 4.8, add GC22014.9U, place the system in a constant temperature water bath with a temperature of 50°C for reaction, and continue enzymatic hydrolysis for 4 hours. place, filter, and remove the precipitate to obtain the ethanol aqueous solution of soybean isoflavone aglycone, evaporate under reduced pressure to remove ethanol (recovery for...

Embodiment 2

[0036] (1) Alkaline ethanol aqueous solution extraction

[0037] Crush the defatted soybean meal and pass through a 60-80 mesh sieve. Take 10g 60~80 mesh defatted soybean meal powder, dissolve in 150ml 70% ethanol solution, Ca(OH) 2 Adjust the pH to 8.0, place the system in a constant temperature water bath at 60°C for extraction, stir and extract for 1 hour, after the extraction is complete, filter with suction to remove the filter residue, and take the supernatant for later use.

[0038] (2) Enzymatic hydrolysis of soybean isoflavones

[0039] Use HCl with a mass fraction of 15% to adjust the pH of the supernatant obtained in step (1) to 4.5, add GC22020.1U, place the system in a constant temperature water bath at 45°C for reaction, and continue the enzymatic hydrolysis for 4.5h. Stand still, filter, remove the precipitate, obtain the ethanol aqueous solution of soybean isoflavone aglycone, evaporate under reduced pressure to remove ethanol (recovery for use), obtain the w...

Embodiment 3

[0047] (1) Alkaline ethanol aqueous solution extraction

[0048] Crush the defatted soybean meal and pass through a 60-80 mesh sieve. Take 10g 60~80 mesh defatted soybean meal powder, dissolve in 200ml 70% ethanol solution, Ca(OH) 2 Adjust the pH to 10.0, place the system in a constant temperature water bath at 50°C for extraction, stir and extract for 2 hours, after the extraction is complete, filter with suction to remove the filter residue, and take the supernatant for later use.

[0049] (2) Enzymatic hydrolysis of soybean isoflavones

[0050] Use HCl with a mass fraction of 15% to adjust the pH of the supernatant obtained in step (1) to 5.0, add GC22018.6U, place the system in a constant temperature water bath at 60°C for reaction, continue enzymatic hydrolysis for 3 hours, and statically place, filter, and remove the precipitate to obtain the ethanol aqueous solution of soybean isoflavone aglycone, evaporate under reduced pressure to remove ethanol (recovery for use), ...

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Abstract

The invention relates to a production method of soybean isoflavone aglycone. An alkaline ethanol water as an extraction agent is subjected to enzyme hydrolysis conversion by beta-glucosidase to effectively obtain soybean isoflavone glycoside; and the soybean isoflavone glycoside is further treated by high purity ethanol to precipitate oligosaccharide, and subjected to ethyl acetate extraction and deionized water washing, so as to refine the soybean isoflavone glycoside and obtain a high purity product. The method can effectively increase extraction rate and reduce the preparation cost. The whole process does not use organic solvent that is toxic and harmful to the human body, and does not discharge acid or alkaline wastewater, so that the whole production process is green, efficient and low-cost, and has good application value.

Description

technical field [0001] The invention relates to a method for extracting refined soybean isoflavone aglycone from defatted soybean meal, in particular to a green and efficient method for preparing high-purity soybean isoflavone aglycone suitable for industrial production. technical background [0002] Epidemiological investigations have found that the incidence of cancer, cardiovascular and other diseases among Southeast Asian populations who eat soybeans for a long time are significantly lower than those in the West. Soybeans and soy products have a wide range of biological activities, such as preventing and treating cancer, lowering blood lipids, anti-atherosclerosis, anti-osteoporosis, anti-senile dementia, and improving women's menopausal syndrome. Studies have shown that these effects are related to the active substances contained in them related to isoflavones. [0003] Soybean isoflavones are a kind of non-sterol substances formed during the growth of soybeans. They a...

Claims

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Application Information

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IPC IPC(8): C12P17/06
Inventor 齐崴王梦凡苏荣欣何志敏郭建鹿洋
Owner TIANJIN UNIV
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