Device and a method for producing biodiesel in a coupling way by treating livestock and poultry breeding wastewater

A technology of livestock and poultry breeding wastewater and biodiesel, which is applied in the field of wastewater treatment, can solve problems such as not being seen, and achieve the effects of simple design, reduced treatment cost, and strong operability

Inactive Publication Date: 2013-05-08
INST OF URBAN ENVIRONMENT CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there has not been any report on the use of photosynthetic bacteria and microalgae in biofuel cells to treat aquaculture wastewater, and to produce algae protein and bio-oil while purifying sewage.

Method used

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  • Device and a method for producing biodiesel in a coupling way by treating livestock and poultry breeding wastewater
  • Device and a method for producing biodiesel in a coupling way by treating livestock and poultry breeding wastewater
  • Device and a method for producing biodiesel in a coupling way by treating livestock and poultry breeding wastewater

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1: Inject livestock and poultry breeding wastewater into the anode chamber through the anode sample inlet, inoculate photosynthetic bacteria in a ratio of 2%-20%, after the inoculation, the airtight anode chamber is isolated from the air, and the anode chamber is used as a fluorescent lamp, an incandescent lamp, an LED lamp or the sun. Light source, the light intensity is controlled at 2000-10000 lux, the temperature is controlled at 23-35°C, and the cathode microalgae produces oxygen through photosynthesis as the electron acceptor. After 50 hours of system treatment, the COD removal rate of livestock and poultry wastewater reaches 94.4%. Under the condition of 1000 Ω, the voltage can reach up to 160 mv ( figure 2 ), the removal rate of ammonia nitrogen from livestock and poultry wastewater was 90.4% ( image 3 ).

Embodiment 2

[0029] Embodiment 2: the anode water outlet is connected with the cathode water inlet, and the livestock and poultry breeding wastewater treated by the anode is jumped to the cathode chamber with a pump, and the microalgae cultivated to the logarithmic growth phase are respectively inoculated in the inoculation amount of 2-20% In the cathode chamber liquid, the concentration of microalgae is about 0.1-0.5 g / L, and the culture temperature is 23°C-35°C. Inject livestock and poultry breeding wastewater into the anode chamber through the anode sample port, inoculate photosynthetic bacteria at a ratio of 2%-20%, use fluorescent lamps, incandescent lamps, LED lamps or the sun as the light source, control the light intensity at 2000-10000 lux, and cultivate the anode water After 13 days of microalgae, the removal rate of ammonia nitrogen was 86.8% ( Figure 4 ) total phosphorus and total nitrogen removal rates were 90.4% and 96.7% ( Figure 5 , Image 6 ), the microalgae cell densi...

Embodiment 3

[0030] Example 3: The oil content of the microalgae in the cathode chamber was 28.2% after 13 days of culture, slightly lower than the oil content of the microalgae in the BG11 culture medium at the same time ( Figure 8 ), the specific determination method is to take 40 mL of algae liquid, centrifuge and concentrate to 0. 8 mL, add 2 mL of methanol and 1 mL of chloroform to it, and mix thoroughly for 2 min. Then add 1 mL of chloroform, mix for 30 s; add 1 mL of water, mix for 30 s, centrifuge at 4000 rpm for 10 min, absorb the entire chloroform layer (bottom layer), and blow with nitrogen at 60°C. Place the blown-dried centrifuge tube at room temperature and dry to constant weight, weigh the mass change before and after the centrifuge tube, that is, the weight of the oil, and the ratio of the weight of the oil to the dry weight of the extracted oil algae can be used to obtain the oil content.

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Abstract

The invention belongs to the technical field of sewage control, and particularly relates to a device and a method for producing biodiesel in a coupling way by treating livestock and poultry breeding wastewater by utilizing an optical microbial fuel cell. The optical microbial fuel cell comprises a light-transmitting anode chamber and a light-transmitting cathode chamber, wherein the light-transmitting anode chamber is used for inoculating photosynthetic bacteria; the light-transmitting cathode chamber is used for inoculating microalgae; the light-transmitting anode chamber and the light-transmitting cathode chamber are spaced through a proton exchange membrane; an anode and a cathode are respectively fixed in the anode chamber and the cathode chamber; the anode chamber is isolated from air to form an anaerobic environment; anode photosynthetic bacteria metabolize organics contained in the livestock and poultry breeding wastewater to generate protons and electrons; the microalgae inoculated in the cathode chamber absorb anode effluent nitrogen and phosphorus to promote the growth of the microalgae; and microalgae photosynthesis provides sufficient dissolved oxygen as an electron acceptor. The device disclosed by the invention enhances the optical utilization ratio by inoculating photosynthetic microorganisms in the anode chamber and the cathode chamber; the photosynthetic bacteria inside the anode chamber can be used for producing mycoprotein; and the microalgae inside the cathode chamber can be used for extracting oil. The method disclosed by the invention has the advantages of good organics, N and P treatment effects, high impact load resistant capacity, high economic benefit, and the like.

Description

technical field [0001] The invention belongs to the field of wastewater treatment. The invention relates to a microbial fuel cell, specifically a double-chamber photosynthetic microbial fuel cell and a device and method for treating livestock and poultry breeding wastewater with low energy consumption. Background technique [0002] Animal husbandry has become one of the three major sources of water environmental pollution. Livestock and poultry excrement has a strong leaching property, and the nitrogen, phosphorus and water-soluble organic matter in the excrement and urine have a large amount of leaching. If not handled properly, it will pass through. Surface runoff and infiltration into the groundwater layer contaminate groundwater. The impact on surface water is mainly manifested in that after a large amount of organic matter enters the water body, the decomposition of the organic matter will consume a large amount of dissolved oxygen in the water and make the water body ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34H01M8/16C10L1/02C12N1/12C12R1/89
CPCY02E50/13Y02E60/527Y02E50/10Y02E60/50Y02W10/37
Inventor 赵峰吴义诚王鲁郑越
Owner INST OF URBAN ENVIRONMENT CHINESE ACAD OF SCI
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