Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation and application of high-purity lysophosphatidylcholine

A phosphatidylcholine, high-purity technology, applied in the field of preparation of high-purity lysophosphatidylcholine, can solve problems such as lack of sufficient research on efficacy and side effects, and achieve the effects of shortening reaction time, reducing reaction cost, and high purity

Active Publication Date: 2013-06-05
FUBICHENG SHANGHAI PHARMA TECH CO LTD
View PDF7 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, there are vitamin D preparations such as calcitonin, but there is still a lack of sufficient research on their efficacy and side effects

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation and application of high-purity lysophosphatidylcholine
  • Preparation and application of high-purity lysophosphatidylcholine
  • Preparation and application of high-purity lysophosphatidylcholine

Examples

Experimental program
Comparison scheme
Effect test

preparation Embodiment 1

[0028] Mix phosphatidylcholine and triglyceride, wherein the mass ratio of phosphatidylcholine and triglyceride is 1:1. The mixture was heated to 85°C to obtain a homogeneous melt. Add a sufficient amount of water and ether to form a solution, wherein the volume ratio of water and ether is 10:1, and heat to 80°C to mix the reactants uniformly. Adjust the pH to 8-9. Add 0.5 ml of an enzyme preparation of LecitaseTM10L phospholipase A2 per kilogram of phosphatidylcholine, and react for 36 hours under the conditions of keeping stirring and pH value until the phosphatidylcholine is completely hydrolyzed. After complete hydrolysis, water and ether were removed by heating to obtain a paste.

[0029] In order to obtain high purity lysophosphatidylcholine, the final product of the reaction is precipitated with acetone, which will separate the two components triglyceride and fatty acid from the above-mentioned hydrolyzate containing lysophosphatidylcholine, triglyceride and fatty aci...

preparation Embodiment 2

[0031] Mix phosphatidylcholine and triglyceride, wherein the mass ratio of phosphatidylcholine and triglyceride is 7:1. The mixture was heated to 85°C to obtain a homogeneous melt. Add a sufficient amount of water and ether to form a solution, wherein the volume ratio of water and ether is 8:1, and heat to 80°C to mix the reactants evenly. Adjust the pH to 8-9. According to every kilogram of phosphatidylcholine, 1ml of an enzyme preparation of LecitaseTM10L, a phospholipase A2, was added, and the mixture was reacted for 36 hours under the condition of stirring and pH value, until the phosphatidylcholine was completely hydrolyzed. After complete hydrolysis, water and ether were removed by heating to obtain a paste.

[0032] In order to obtain high purity lysophosphatidylcholine, the final product of the reaction is precipitated with acetone, which will separate the two components triglyceride and fatty acid from the above-mentioned hydrolyzate containing lysophosphatidylcholi...

preparation Embodiment 3

[0034] Shell the castor beans, smash them, and then degrease: add 4mL of diethyl ether and petroleum ether mixture per gram of castor beans and mix evenly, then use the Buchner funnel to suction filter, and then use the above degreasing method to degrease the material on the filter paper 3 times, Then the components on the filter paper are dried to obtain the loose castor-oil biscuit powder after oiling, for subsequent use; the mixed solution of diethyl ether and sherwood oil, wherein the volume ratio of diethyl ether and sherwood oil is 1:1;

[0035] Wrap the prepared castor biscuit powder with filter paper, put it into a Soxhlet extractor, add ethanol with a mass concentration of 95% according to the ratio of adding 10 mL of ethanol per gram of castor biscuit powder, extract for 8 hours, and obtain a yellow-brown oily substance for extraction Liquid, and then distilled to 1 / 5 of the original volume with a rotary evaporator to obtain a paste, and then according to the ratio of...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a novel effective method for preparing high-purity lysophosphatidylcholine, and a purpose for applying the high-purity lysophosphatidylcholine for preventing and / or treating metabolic bone diseases. According to the novel preparation method, phosphatidylcholine is converted into lysophosphatidylcholine under the catalysis of phospholipase A2. During the conversion process, a novel phospholipase A2 activity enhancer is added. Therefore, phospholipase A2 dose is reduced, lysophosphatidylcholine yield is improved, and high-purity lysophosphatidylcholine can be prepared. The prepared high-purity lysophosphatidylcholine can be used in medicine compositions used for preventing and / or treating metabolic bone diseases.

Description

technical field [0001] The invention relates to a preparation method and application of high-purity lysophosphatidylcholine. technical background [0002] Lysophosphatidylcholine is the product obtained by removing a fatty acyl group from phosphatidylcholine. Phosphatidylcholine (also known as lecithin) is widely present in animals and plants, and is more abundant in the brain, adrenal gland and cells of animals. Compared with phosphatidylcholine, lysophosphatidylcholine has a larger HLB value, which not only retains lipophilicity, but also increases its hydrophilicity due to the reduction of non-polar groups. Abilities are superior to phosphatidylcholine. Lysophosphatidylcholine plays an important role in improving the texture of bakery products. Lysophosphatidylcholine also has biological activity and plays an important role in the prevention, diagnosis and treatment of atherosclerosis. At present, lysophosphatidylcholine is more and more widely used in food, cosmetics...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12P13/00A61K31/685A61P19/10A61P19/08A61P35/00A61P35/04A61P19/02A61P1/02
Inventor 曹亮
Owner FUBICHENG SHANGHAI PHARMA TECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com